Study on the soil microbial community structure associated with six land use in Siding mining area
YU Fang-ming1,2, YAO Ya-wei2, XIE Dong-yu2, WANG Xue-ru2, LIN Jia-min2, LIU Yuan2, LIU Ke-hui1,3, LI Yi1,2
1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin 541004, China; 2. College of Environment and Resources, Guangxi Normal University, Guilin 541004, China; 3. College of Life Science, Guangxi Normal University, Guilin 541004, China
Abstract：Six different land use types (corn field, vegetable field, shrubs, mine tailing, forests and citrus orchards) were selected as variables around the Siding Lead-Zinc mining area in Liuzhou, Guangxi Province in the present study. The soil bacterial abundance, diversity and community composition were examined using Illumina MiSeq high-throughput sequencing technology to discuss the effects of soil physicochemical properties on soil microbial community structure. The results indicated that phylum Proteobacteria was the most dominant taxon in these land use types, with a high abundance ranging from 31.34% to 53.47%. Gammaproteobacteria was the most dominant class. The redundancy analysis showed that in shrubs where the content of heavy metals in the soil was the highest, there was a significant positive correlation between the relative abundance of Firmicutes and Bacteroidetes and the content of lead, zinc, cadmium and copper in soils. Additionally, in corn and vegetable field with high levels soil sodium and potassium contents, the relative abundance of Planctomycetes increased, while the relative abundance of Firmicutes and Bacteroidetes decreased. Moreover, in citrus orchards with high soil organic matter content and low nitrogen and phosphorus content, the relative abundance of Acidobacteria and Verrucomicrobia were comparably high. There was a significant positive correlation between the relative abundance of Gemmatimonadetes and Planctomycetes and the soil pH. Hence, the results indicated that land use and soil environmental factors had significant impact on the diversity of soil microbial community structure.
于方明, 姚亚威, 谢冬煜, 王雪茹, 林嘉敏, 刘媛, 刘可慧, 李艺. 泗顶矿区6种土地利用类型土壤微生物群落结构特征[J]. 中国环境科学, 2020, 40(5): 2262-2269.
YU Fang-ming, YAO Ya-wei, XIE Dong-yu, WANG Xue-ru, LIN Jia-min, LIU Yuan, LIU Ke-hui, LI Yi. Study on the soil microbial community structure associated with six land use in Siding mining area. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(5): 2262-2269.
张双双,靳振江,贾远航,等.岩溶地区不同土地利用方式土壤固碳细菌群落结构特征[J]. 环境科学, 2019,40(1):412-420. Zhang S S, Jin Z J, Jia Y H, et al. Community structure of CO2-fixing soil bacteria from different land use types in karst areas[J]. Environmental Science, 2019,40(1):412-420.
[2]
于方明,余秋平,刘可慧,等.肠杆菌FM-1强化积雪草修复镉污染土壤机理[J]. 中国环境科学, 2018,38(12):227-232. Yu F M, Yu Q P, Liu K H, et al. Improvement of cadmium-contaminated soil phytoremediation by Centella asiatica L. through bioaugmentation of Enterobacter sp. FM-1[J]. China Environmental Science, 2018,38(12):227-232.
[3]
Xun Y, Feng L, Li Y, et al. Mercury accumulation plant Cyrtomium macrophyllum and its potential for phytoremediation of mercury polluted sites[J]. Chemosphere, 2017,189:161-170.
[4]
Wang S, Liu W, Zhao S, et al. Denitrification is the main microbial N loss pathway on the Qinghai-Tibet Plateau above an elevation of 5000mm[J]. Science of The Total Environment, 2019,696:133852.
[5]
安申群,贡璐,李杨梅,等.塔里木盆地北缘绿洲4种土地利用方式土壤有机碳组分分布特征及其与土壤环境因子的关系[J]. 环境科学, 2018,39(7):3383-3390. An S Q, Gong L, Li Y M, et al. Soil organic carbon components and their correlation with soil physicochemical factors in four different land use types of the northern Tarim Basin[J]. Environmental Science, 2018,39(7):3383-3390.
[6]
楚海燕,李若南,李靖雯,等.中亚热带森林转换对土壤微生物群落结构的影响[J]. 应用与环境生物学报, 2019,25(1):23-28. Chu H Y, Li R N, Li J W, et al. Effects of forest conversion on soil microbial community structure[J]. Chinese Journal of Applied and Environmental Biology, 2019,25(1):23-28.
[7]
郑涵,田昕竹,王学东,等.锌胁迫对土壤中微生物群落变化的影响[J]. 中国环境科学, 2017,37(4):1458-1465. Zheng H, Tian X Z, Wang X D, et al. Effects of Zn pollution on soil microbial community in field soils and its main influence factors[J]. China Environmental Science, 2017,37(4):1458-1465.
[8]
贺文员,宋清晖,杨尚霖,等.生物有机肥对水稻土壤酶活性及微生物群落结构的影响[J]. 中国农学通报, 2019,35(27):106-113. He W Y, Song Q H, Yang S L, et al. Biological fertilizer:effects on enzyme activity and microbial community structure in rice soil[J]. Chinese Agricultural Science Bulletin, 2019,35(27):106-113.
[9]
Zhang C, Nie S, Liang J, et al. Effects of heavy metals and soil physicochemical properties on wetland soil microbial biomass and bacterial community structure[J]. Science of The Total Environment, 2016,557-558:785-790.
[10]
Li W, Liu M, Wu M, et al. Bacterial community succession in paddy soil depending on rice fertilization[J]. Applied Soil Ecology, 2019, 144:92-97.
[11]
Rofner C, Peter H, Catalán N, et al. Climate-related changes of soil characteristics affect bacterial community composition and function of high altitude and latitude lakes[J]. Global Change Biology, 2017,23(6):2331-2344.
[12]
Oka M, Uchida Y Heavy metals in slag affect inorganic N dynamics and soil bacterial community structure and function[J]. Environmental Pollution, 2018,243:713-722.
[13]
Brueck W M, Brueck T B, Self W T, et al. Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the straits of Florida[J]. The ISME Journal, 2010,4(5):686-699.
[14]
Yu X, Kang X, Li Y, et al. Rhizobia population was favoured during in situ phytoremediation of vanadium-titanium magnetite mine tailings dam using Pongamia pinnata[J]. Environmental Pollution, 2019,255:113167.
[15]
鲁如坤.土壤农业化学分析法[M]. 北京:中国农业科技出版社, 1999. Lu R K. Agrochemical analysis method of soil[M]. Beijing:China Agricultural Science and Technology Press, 1999.
[16]
Edgar R C. UPARSE:highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods, 2013,10(10):996-998.
[17]
靳希桐,胡文革,贺帅兵,等.不同时期艾比湖湿地盐角草群落土壤固氮微生物的多样性分析[J]. 微生物学报, 2019,59(8):1600-1611. Jin X T, Hu W G, He S B, et al. Diversity of soil nitrogen-fixing microorganisms in Salicorniaeuropaea community of Ebinur Lake wetland during differentperiods[J]. Acta Microbiologica Sinica, 2019, 59(8):1600-1611.
[18]
Song H, Peng L, Li Z, et al. Metal distribution and biological diversity of crusts in paddy fields polluted with different levels of cadmium[J]. Ecotoxicology and Environmental Safety, 2019,184:109620.
[19]
Kevin F, Philip R, Chris F, et al. Differences in hyporheic-zone microbial community structure along a heavy-metal contamination gradient[J]. Applied & Environmental Microbiology, 2003,69(9):5563-5573.
[20]
Li Y, Chen L, Wen H, et al. 454Pyrosequencing analysis of bacterial diversity revealed by a comparative study of soils from mining subsidence and reclamation areas[J]. Journal of Microbiology and Biotechnology, 2014,24(3):313-323.
[21]
Spain A M, Krumholz L R, Elshahed M S. Abundance, composition, diversity and novelty of soil Proteobacteria[J]. The ISME Journal, 2009,3(8):992-1000.
[22]
陈承利,廖敏,曾路生污染土壤微生物群落结构多样性及功能多样性测定方法[J]. 生态学报, 2006,26(10):3404-3412. Chen C L, Liao M, Zeng L S. Methods to measure the microbial community structure and functional diversity inpolluted soils[J]. Acta Ecologica Sinica, 2006,26(10):3404-3412.
[23]
James A L, Chilvers K F, Perry J D, et al. Evaluation of p-naphtholbenzein-β-D-galactoside as a substrate for bacterial β-galactosidase[J]. Applied and Environmental Microbiology, 2000, 66(12):5521-5523.
[24]
Fuerst J A, Sagulenko E. Beyond the bacterium:planctomycetes challenge our concepts of microbial structure and function[J]. Nature Reviews Microbiology, 2011,9(6):403-413.
[25]
Kenarova A, Radeva G, Traykov I, et al. Community level physiological profiles of bacterial communities inhabiting uranium mining impacted sites[J]. Ecotoxicology and Environmental Safety, 2014,100:226-232.
[26]
Stemmer M, Watzinger A, Blochberger K, et al. Linking dynamics of soil microbial phospholipid fatty acids to carbon mineralization in a 13C natural abundance experiment:Impact of heavy metals and acid rain[J]. Soil Biology and Biochemistry, 2007,39(12):3177-3186.
[27]
Narihiro T, Tamaki H, Akiba A, et al. Microbial community structure of relict niter-beds previously used for saltpeter production[J]. Plos One, 2014,9(8):e104752.
[28]
Kim J M, Roh A S, Choi S C, et al. Soil pH and electrical conductivity are key edaphic factors shaping bacterial communities of greenhouse soils in Korea[J]. Journal of Microbiology, 2016,54(12):838-845.
[29]
Tripathi B M, Kim M, Singh D, et al. Tropical soil bacterial communities in Malaysia:pH dominates in the equatorial tropics too[J]. Microbial Ecology, 2012,64(2):474-484.
[30]
Liu J, Sui Y, Yu Z, et al. High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China[J]. Soil Biology and Biochemistry, 2014,70:113-122.
[31]
Hollister E B, Engledow A S, Hammett A J M, et al. Shifts in microbial community structure along an ecological gradient of hypersaline soils and sediments[J]. The ISME Journal, 2010,4(6):829-838.
[32]
Sul W J, Asuming-Brempong S, Wang Q, et al. Tropical agricultural land management influences on soil microbial communities through its effect on soil organic carbon[J]. Soil Biology and Biochemistry, 2013,65:33-38.
[33]
Huang Q, Wang J, Wang C, et al. The 19-years inorganic fertilization increased bacterial diversity and altered bacterial community composition and potential functions in a paddy soil[J]. Applied Soil Ecology, 2019,144:60-67.
