Improving fermentative hydrogen production of anaerobic sludge by redox mediators
ZHANG Li-guo1,2, AI Bing-ling3, LI Jian-zheng4, BAN Qiao-ying1,2
1. College of Environment and Resource Sciences, Shanxi University, Taiyuan 030006, China; 2. Shanxi Laboratory for Yellow River, Taiyuan 030006, China; 3. Haikou Experimental Station, Chinese Academy Tropical Agricultural Sciences, Haikou 571101, China; 4. School of Environment, Harbin Institute of Technology, Harbin 150090, China
Abstract：To improve the hydrogen production activity of anaerobic sludge, the effects of redox mediators (ROMs) on the hydrogen production efficiency of anaerobic sludge using glucose as substrate were investigated by batch culture. The microbial community structure was revealed by Illumina MiSeq sequencing. The results showed that the cumulative hydrogen production and maximum hydrogen production rate (Rmax) of the control were respectively 11.0mL and 0.28mL/h under conditions of the fermentative volume of 100mL and the initial glucose concentration of 500mg/L. When humic acid and anthraquinone-2-sulfonic acid (AQS) as ROMs were added to the system, the hydrogen production capacity of anaerobic sludge was significantly improved. The cumulative hydrogen production was higher than the control by 56.4% and 13.6%, respectively. While Rmax was increased by 53.6% and 10.7%, respectively. On the contrary, the hydrogen production capacity of anaerobic sludge was inhibited when graphene oxide (GO), hennaquinone and anthraquinone-2,6-disulfonic acid (AQDS) were used as ROMs. Illumina MiSeq sequencing revealed that the dominant microbial groups from each sample were different. Thermomarinilinea, Longilinea, Defluviimonas, Gimesia and Planctomicrobium were the dominant genera in control. The major genera were shifted to Thermomarinilinea, Longilinea and Ottowia in humic acid, AQS and hennaquinone fermentation systems. While Thermomarinilinea, Longilinea, Ottowia and Defluviimonas became the predominant genera in AQDS and GO fermentation systems. Redundancy analysis indicated that hydrogen production was positively correlated with Longilinea, Macellibacteroides, Trichococcus, Thioflavicoccus and Ottowia, whereas negatively correlated with Defluviimonas, Pirellula, Gimesia and Ignavibacterium.
张立国, 艾冰凌, 李建政, 班巧英. 氧化还原介体强化厌氧活性污泥发酵产氢特征[J]. 中国环境科学, 2021, 41(5): 2196-2202.
ZHANG Li-guo, AI Bing-ling, LI Jian-zheng, BAN Qiao-ying. Improving fermentative hydrogen production of anaerobic sludge by redox mediators. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(5): 2196-2202.
王海燕,郝瑞霞,赵雅琪,等.蜡样芽孢杆菌中甲酸脱氢酶基因产氢研究[J]. 中国环境科学, 2018,38(2):729-736. Wang H Y, Hao R X, Zhao Y Q, et al. Studies on hydrogen production via formate dehydrogenase from Bacillus cereus strains[J]. China Environmental Science, 2018,38(2):729-736.
[2]
杜金宇,任学勇,青春耀,等.厌氧混合菌群生物共发酵产氢动力学研究[J]. 农业机械学报, 2019,50(3):294-299. Du J Y, Ren X Y, Qing C Y, et al. Dynamics characteristics of co-fermentative hydrogen production by mixed photosynthentic and anaerobic consortium[J]. Transactions of the Chinese Society for agricultural Machinery, 2019,50(3):294-299.
[3]
Zhang Q, Wang Y, Zhang Z, et al. Photo fermentative hydrogen production from crop residue:a mini review[J]. Bioresource Technology, 2017,229:222-230.
[4]
Arimi M M, Knodel J, Kiprop A, et al. Strategies for improvement of biohydrogen production from organic rich wastewater:a review[J]. Biomass and Bioenergy, 2015,75:101-118.
[5]
班巧英,刘琦,余敏,等.氧化还原介体催化强化污染物厌氧降解研究进展[J]. 科技导报, 2019,37(21):88-96. Ban Q Y, Liu Q, Yu M, et al. Review on catalytic effects of redox mediator in anaerobic degradation of pollutants[J]. Science and Technology Review, 2019,37(21):88-96.
[6]
丁惠君,吴亦潇,钟家有,等.两种介体物质在漆酶降解磺胺类抗生素中的作用[J]. 中国环境科学, 2016,36(5):1469-1475. Ding H J, Wu Y X, Zhong J Y, et al. Role of two mediators in sulfonamide antibiotics degradation by laccase oxidation system[J]. China Environmental Science, 2016,36(5):1469-1475.
[7]
Zhang D, Zhang N, Yu X et al. Effect of humins from different sediments on microbial degradation of 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153), and their polyphasic characterization[J]. Rsc Advances, 2017,7(12):6849-6855.
[8]
班巧英,岳立峰,李建政,等.萘厌氧降解菌群的富集及氧化还原介体的强化[J]. 中国环境科学, 2020,40(7):3150-3155. Ban Q Y, Yue L F, Li J Z, et al. Enrichment of naphthalene anaerobic degrading bacterial consortium and enhancement by redox mediators[J]. China Environmental Science, 2020,40(7):3150-3155.
[9]
国家环保局《水和废水监测分析方法》编委会.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002:27-30. State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2002:27-30.
[10]
张立国,班巧英,李建政.UASB反应器中产甲烷菌对温度胁迫的响应[J]. 中国环境科学, 2016,36(4):1082-1086. Zhang L G, Ban Q Y, Li J Z. Response of methanogens on temperature stress in an UASB reactor[J]. China Environmental Science, 2016,36(4):1082-1086.
[11]
Martinez C M, Alvarez L H, Celis L B et al. Humus-reducing microorganisms and their valuable contribution in environmental processes[J]. Applied Microbiology and Biotechnology, 2013,97(24):10293-10308.
[12]
Abubackar H N, Keskin T, Yazgin O, et al. Biohydrogen production from autoclaved fruit and vegetable wastes by dry fermentation under thermophilic condition[J]. International Journal of hydrogen Energy, 2019,44:18776-18784.
[13]
Qin Y, Li L, Wu J, et al. Co-production of biohydrogen and biomethane from food waste and paper waste via recirculated two-phase anaerobic digestion process:Bioenergy yields and metabolic distribution[J]. Bioresource Technology, 2019,276:325-334.
[14]
Antwi P, Li J, Boadi P O, et al. Functional bacterial and archaeal diversity revealed by 16S rRNA gene pyrosequencing during potato starch processing wastewater treatment in an UASB[J]. Bioresource Technology, 2017,235:348-357.
[15]
Wang J, Ruan C, Song L, et al. Gimesia benthica sp.nov., a planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean[J]. International Journal of Systematic and Evolutionary Microbiology, DOI:10.1099/ijsem.0.004301.
[16]
Kulichevskaya I S, Ivanova A A, Detkova E N, et al. Planctomicrobium piriforme gen. nov., sp. nov., a stalked planctomycete from a littoral wetland of a boreal lake[J]. International Journal of Systematic and Evolutionary Microbiology 2015,65:1659-1665.
[17]
Yamada T, Imachi H, Ohashi A, et al. Bellilinea caldifistulae gen. nov., sp. nov. and Longilinea arvoryzaegen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading consortia[J]. International Journal of Systematic and Evolutionary Microbiology, 2007,57(10):2299-2306.
[18]
Nunoura T, Hirai M, Miyazaki M, et al. Isolation and characterization of a thermophilic, obligately anaerobic and heterotrophic marine Chloroflexi bacterium from a Chloroflexi-dominated microbial community associated with a Japanese Shallow Hydrothermal system, and proposal for Thermomarinilinea lacunofontalis gen. nov., sp. nov[J]. Microbes and Environments, 2013,28(2):228-235.
[19]
Spring S, Jäckel U, Wagner M, et al. Ottowia thiooxydans gen. nov., sp. nov., a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen.nov., comb. nov[J]. International Journal of Systematicand Evolutionary Microbiology, 2004,54:99-106.
[20]
Strepis N, Sánchez-Andrea I, van Gelder AH, et al. Description of Trichococcus ilyis sp.nov.by combined physiological and in silicogenome hybridization analyses[J]. International Journal of Systematic and Evolutionary Microbiology, 2016,66(10):3957-3963.
[21]
Jabari L, Gannoun H, Cayol J L, et al. Macellibacteroides fermentans gen.nov.,sp.nov., A member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters[J]. International Journal of Systematic and Evolutionary Microbiology, 2012,62:2522-2527.
[22]
Jones J R, Massanet-Nicolau J, Mulder M J J. Increased biohydrogen yields, volatile fatty acid production and substrate utilisation rates via the electrodialysis of a continually fed sucrose fermenter[J]. Bioresource Technology, 2017,229:46-52.
