Abstract:Through three kinds of carbon source (HCOONa, CH3COONa and C6H12O6), under different concentrations of Desulfovibrio desulfuricans sub sp.(D. desulfuricans sp.) for stress/induction training, the changes in EPS fractions before and after stress/induction and changes in the sorption properties of Cd(II) were investigated. The results showed that when the stress concentration of HCOONa was 3.0g/L, the stress/induction effect was the most obvious, EPS production reached 1709.78mg/g VSS, and the protein yield in EPS was the highest, reaching 1516.68mg/g VSS, which was 244.93% higher than that before stress/induction. Under these conditions, HCOONa-EPS showed the highest adsorption of Cd(II) at 1081.95mg/g EPS, an increase of 99.47%, and the adsorption process conforms to the secondary kinetic law. Three-dimensional fluorescence (3D-EEM) tests showed that the protein content, especially tryptophan-like content, increased significantly in EPS after stress/induction. Fourier infrared spectroscopy (FTIR) test showed that C=O, N-H/C-N, C-O-C and other functional groups increased greatly after stress, which played an important role in adsorption of Cd(II). X-ray electron spectroscopy (XPS) showed that after the stress/induction of carbon source HCOONa, the concentration of functional groups such as C-O/C-N, C=O, C=N and other functional groups in EPS increased significantly, which may be the main group for adsorption of Cd(II).
黄祥武, 宋卫锋, 杨佐毅, 戴文灿, 甘雨, 连泽阳, 周文斌, 陈丽瑶, 吴芷昕. 碳源诱导下D. desulfuricans sub sp. EPS特性及对Cd(Ⅱ)的吸附[J]. 中国环境科学, 2023, 43(6): 2889-2898.
HUANG Xiang-wu, SONG Wei-feng, YANG Zuo-yi, DAI Wen-can, GAN Yu, LIAN Ze-yang, ZHOU Wen-bin, CHENG Li-yao, WU Zhi-xin. The EPS characteristics of D. desulfuricans sub sp. and its adsorption performance for Cd(Ⅱ) under carbon source induction. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(6): 2889-2898.
Harimawan A, Ting Y P. Investigation of extracellular polymeric substances (EPS) properties of P. aeruginosa and B. subtilis and their role in bacterial adhesion[J]. Colloids and Surfaces B:Biointerfaces, 2016, 146:459-467.
[2]
Ha J, Gélabert A, Spormann A M, et al. Role of extracellular polymeric substances in metal ion complexation on Shewanella oneidensis:Batch uptake, thermodynamic modeling, ATR-FTIR, and EXAFS study[J]. Geochimica et Cosmochimica Acta, 2010,74(1):1-15.
[3]
Guibaud G, Van Hullebusch E, Bordas F. Lead and cadmium biosorption by extracellular polymeric substances (EPS) extracted from activated sludges:pH-sorption edge tests and mathematical equilibrium modelling[J]. Chemosphere, 2006,64(11):1955-1962.
[4]
Izadi P, Eldyasti A. Holistic insights into extracellular polymeric substance (EPS) in anammosx bacterial matrix and the potential sustainable biopolymer recovery:A review[J]. Chemosphere, 2021, 274:129703.
[5]
朱广森,汪文军,吴 峥,等.微生物诱导碳酸盐沉积介导的Cd2+固定[J]. 中国环境科学, 2021,41(12):5912-5920. Zhu G S, Wang W J, Wu Z, et al. Microbially induced Cd2+ fixation mediated by carbonate deposition[J]. China Environmental Science, 2021,41(12):5912-5920.
[6]
曾峤婧,周 鑫,黄 超,等.白腐菌联合纳米零价铁强化去除水中Cd(Ⅱ)[J]. 中国环境科学, 2022,42(7):3174-3183. Zeng Q J, Zhou X, Huang C, et al. Enhanced removal of Cd(II) from aqueous solution by nanoscale zero-valent iron coupled with white rot fungus[J]. China Environmental Science, 2022,42(7):3174-3183.
[7]
连泽阳,杨佐毅,宋卫锋,等.外源Cd(Ⅱ)胁迫Alcaligenes faecalis过程中阴离子对EPS产量及其特性的影响[J]. 环境科学学报, 2022, 42(4):81-90. Lian Z Y, Yang Z Y, Song W F, et al. Effect of anions on EPS yield and characteristics during exogenous Cd(II) stress Alcaligenes faecalis[J]. Journal of Environmental Science, 2022,42(4):81-90.
[8]
Li J, Jiang Z, Chen S, et al. Biochemical changes of polysaccharides and proteins within EPS under Pb(II) stress in Rhodotorula mucilaginosa[J]. Ecotoxicology and Environmental Safety, 2019, 174:484-490.
[9]
Li Q H, Song W F, Sun M G, et al. Composition change and adsorption performance of EPS from Bacillus vallismortis sp. induced by Na2S[J]. Ecotoxicology and Environmental Safety, 2019,185:109679.
[10]
甘 雨,宋卫锋,杨佐毅,等.外源硫诱导下的Desulfovibrio desulfuricans sub sp. EPS特性及对Zn(Ⅱ)的吸附[J]. 中国环境科学, 2022,42(11):5144-5152. Gan Y, Song W F, Yang Z Y, et al. Desulfovibrio desulfuricans sub sp. EPS characteristics and its adsorption of Zn(II) under exogenous sulfur induction[J]. China Environmental Science, 2022,42(11):5144-5152.
[11]
Premnath N, Mohanrasu K, Guru Raj Rao R, et al. Effect of C/N substrates for enhanced extracellular polymeric substances (EPS) production and Poly Cyclic Aromatic Hydrocarbons (PAHs) degradation[J]. Environmental Pollution, 2021,275:116035.
[12]
Chen Y, Xu Y N. Advances in heavy metal removal by sulfate-reducing bacteria[J]. Water Science and Technology, 2020,81(9):1797-1827.
[13]
Cao J, Zhang G, Mao Z S, et al. Influence of electron donors on the growth and activity of sulfate-reducing bacteria[J]. International Journal of Mineral Processing, 2012,106-109:58-64.
[14]
Li J, Ran X, Zhou M, et al. Oxidative stress and antioxidant mechanisms of obligate anaerobes involved in biological waste treatment processes:A review[J]. Science of The Total Environment, 2022,838(Pt 3):156454.
[15]
万正强. EPS在硫酸盐脱硫弧菌(Desulfovibrio desulfuricans)去除重金属Cd2+过程作用研究[D]. 合肥:合肥工业大学, 2013. Wan Z Q. Study on the role of EPS in the removal of heavy metals Cd2+ by Desulfovibrio desulfuricans[D]. Hefei:Hefei University of Technology, 2013.
[16]
Felz S, Vermeulen P, Van Loosdrecht M C M, et al. Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS)[J]. Water Research, 2019,157:201-208.
[17]
Shen L, Cheng J, Wang J, et al. Comparison of extraction methods for extracellular polymeric substances (EPS) and dynamic characterization of EPS from sessile microorganisms during pyrite bioleaching[J]. Journal of Environmental Chemical Engineering, 2022,10(3):107922.
[18]
Tang Y, Dai X, Dong B, et al. Humification in extracellular polymeric substances (EPS) dominates methane release and EPS reconstruction during the sludge stabilization of high-solid anaerobic digestion[J]. Water Research, 2020,175:115686.
[19]
Santos J C D, Lopes D R G, Silva L C F, et al. Characterization of the biofilm structure and microbial diversity of sulfate-reducing bacteria from petroleum produced water supplemented by different carbon sources[J]. Journal of Environmental Management, 2022,304:114189.
[20]
Xu R Z, Cao J S, Feng G, et al. Fast identification of fluorescent components in three-dimensional excitation-emission matrix fluorescence spectra via deep learning[J]. Chemical Engineering Journal, 2022,430:132893.
[21]
Xie T, Xi Y, Liu Y, et al. Long-term effects of Cu(II) on denitrification in hydrogen-based membrane biofilm reactor:Performance, extracellular polymeric substances and microbial communities[J]. Science of The Total Environment, 2022,830:154526.
[22]
Fan Y, Su J, Zheng Z, et al. Denitrification performance and mechanism of a novel isolated Acinetobacter sp. FYF8in oligotrophic ecosystem[J]. Bioresource Technology, 2021,320(Pt A):124280.
[23]
Yin Y, Hu Y, Xiong F. Sorption of Cu(II) and Cd(II) by extracellular polymeric substances (EPS) from Aspergillus fumigatus[J]. International Biodeterioration & Biodegradation, 2011,65(7):1012-1018.
[24]
Ueshima M, Ginn B R, Haack E A, et al. Cd adsorption onto Pseudomonas putida in the presence and absence of extracellular polymeric substances[J]. Geochimica et Cosmochimica Acta, 2008, 72(24):5885-5895.
[25]
Ferreira M L, Gerbino E, Cavallero G J, et al. Infrared spectroscopy with multivariate analysis to interrogate the interaction of whole cells and secreted soluble exopolimeric substances of Pseudomonas veronii 2E with Cd(II), Cu(II) and Zn(II)[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2020,228:117820.
[26]
Guibaud G, Tixier N, Bouju A, et al. Relation between extracellular polymers' composition and its ability to complex Cd, Cu and Pb[J]. Chemosphere, 2003,52(10):1701-1710.
[27]
Shukla A, Parmar P, Goswami D, et al. Characterization of novel thorium tolerant Ochrobactrum intermedium AM7in consort with assessing its EPS-Thorium binding[J]. Journal of Hazardous Materials, 2020,388:122047.
[28]
Parikh S J, Mukome F N, Zhang X. ATR-FTIR spectroscopic evidence for biomolecular phosphorus and carboxyl groups facilitating bacterial adhesion to iron oxides[J]. Colloids and Surfaces B:Biointerfaces, 2014, 119:38-46.
[29]
赵 菲.碳源对自絮凝微藻Neocystis mucosa SX的胞外聚合物特征的影响研究[D]. 太原:山西大学, 2020. Zhao F. Effect of carbon source on extracellular polymer characteristics of Neocystis mucosa SX of self-flocculating microalgae[D]. Taiyuan:Shanxi University, 2020.
[30]
Li Y P, You L X, Yang X J, et al. Extrapolymeric substances (EPS) in Mucilaginibacter rubeus P2displayed efficient metal(loid) bio-adsorption and production was induced by copper and zinc[J]. Chemosphere, 2022,291(Pt 1):132712.
[31]
Lian Z Y, Yang Z Y, Song W F, et al. Effects of different exogenous cadmium compounds on the chemical composition and adsorption properties of two gram-negative bacterial EPS[J]. Science of The Total Environment, 2022,806(Pt 1):150511.
[32]
Zhang L, Zhao Q, Zhang M, et al. Mg2+ distribution in activated sludge and its effects on the nitrifying activity and the characteristics of extracellular polymeric substances and sludge flocs[J]. Process Biochemistry, 2020,88:120-128.
[33]
Xia L, Tan J, Wu P, et al. Biopolymers extracted from Klebsiella sp. and Bacillus sp. in wastewater sludge as superb adsorbents for aqueous Hg(II) removal from water[J]. Chemical Physics Letters, 2020,754:137689.
[34]
Atrei A, Lesiak Orlowska B, Tóth J. Magnetite nanoparticles functionalized with citrate:A surface science study by XPS and ToF-SIMS[J]. Applied Surface Science, 2022,602:154366.
[35]
Luo X, Zhou X, Peng C, et al. Bioreduction performance of Cr(VI) by microbial extracellular polymeric substances (EPS) and the overlooked role of tryptophan[J]. Journal of Hazardous Materials, 2022,433:128822.
[36]
Wang L, Wu Y, Ren Y, et al. Transition of fouling characteristics after development of membrane wetting in membrane-aerated biofilm reactors (MABRs)[J]. Chemosphere, 2022,299:134355.
[37]
Han J, Pei L, Du Y, et al. Tripolycyanamide-2,4,6-triformyl pyrogallol covalent organic frameworks with many coordination sites for detection and removal of heavy metal ions[J]. Journal of Industrial and Engineering Chemistry, 2022,107:53-60.