新型连续流系统中Anammox颗粒粒径控制策略研究

李冬; 李崧岳; 董怡雯; 蒋鹏飞; 李帅; 曾辉平; 张杰

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中国环境科学 ›› 2024, Vol. 44 ›› Issue (8) : 4355-4365.

水污染与控制

新型连续流系统中Anammox颗粒粒径控制策略研究

李冬¹, 李崧岳¹, 董怡雯¹, 蒋鹏飞¹, 李帅¹, 曾辉平¹, 张杰^1,2

作者信息 +

Study on granule size control strategies in a novel continuous flow system for Anammox

LI Dong¹, LI Song-yue¹, DONG Yi-wen¹, JIANG Peng-fei¹, LI Shuai¹, ZENG Hui-ping¹, ZHANG Jie^1,2

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摘要

以具有自主专利的新型大/小双室自回流连续流反应器为平台,探究基于粒径回流的Anammox颗粒污泥粒径调控策略及调控机制.粒径回流策略通过改变内循环的位置,将不同粒径的污泥引入高基质浓度和高机械剪切区域进行粒径调控.结果表明,粒径回流策略能够提高Anammox颗粒粒径,并将其控制在合适的范围内.这一策略还增强了系统的污泥浓度、颗粒沉降性能和脱氮性能.其中大颗粒回流策略综合提升效果最佳,维持颗粒粒径在0.5~1.6mm范围内,中值粒径为948μm,污泥体积指数(SVI)为35.6mL/g,总无机氮(TIN)去除率为88.59%.胞外聚合物(EPS)分析和分层实验表明,紧密结合EPS(TB-EPS)中的蛋白质(PN)在粒径调控中起着关键作用,高浓度的外层污泥PN主导了颗粒的沉降性能,而内层紧实污泥的TB-EPS中的PN则增强了颗粒的结构强度.高通量测序结果表明,粒径循环策略能够大幅提高系统内厌氧氨氧化菌(AnAOB)Candidatus Kuenenia的丰度,在大颗粒回流时达到60.24%的峰值,并且AnAOB主要分布在颗粒污泥内层.

Abstract

This study, employed a novel, patented twin-reactor self-recirculating system to explore the granule size control strategies and its controlling mechanisms of anaerobic ammonium oxidation (Anammox) granular sludge based on granules size recirculation approach. By adjusting internal recirculation locations, the strategy aimed to control the size of granules through introducing sludge of varying sizes into regions with high substrate concentration and mechanical shear. The result demonstrated that this strategy effectively increased granule size, and maintained it within an appropriate range. Furthermore, it enhanced sludge concentration, settling characteristics, and nitrogen removal performance. Specifically, recirculating larger granules proved to be highly effective, keeping the size of granular sludge within the optimal range of 0.5~1.6mm, with a median size of 948μm, SVI of 35.6mL/g, and an enhanced total inorganic nitrogen (TIN) removal efficiency of 88.59%. Analysis of extracellular polymeric substances (EPS) revealed that proteins (PN) in the tightly bound EPS (TB-EPS) played a crucial role in granule size control. Stratification experiments revealed that PN in the outer layer of the sludge primarily influenced settleability, while PN in the TB-EPS of the inner layer strengthens the granule structure. High-throughput sequencing showed a significant increase in the abundance of anammox bacteria (AnAOB) Candidatus Kuenenia, mainly located in the inner layers of the sludge granules. These findings have significant implications for the engineering application of anammox granular sludge technology.

导出引用

李冬, 李崧岳, 董怡雯, 蒋鹏飞, 李帅, 曾辉平, 张杰. 新型连续流系统中Anammox颗粒粒径控制策略研究[J]. 中国环境科学. 2024, 44(8): 4355-4365

LI Dong, LI Song-yue, DONG Yi-wen, JIANG Peng-fei, LI Shuai, ZENG Hui-ping, ZHANG Jie. Study on granule size control strategies in a novel continuous flow system for Anammox[J]. China Environmental Science. 2024, 44(8): 4355-4365

中图分类号： X703.1

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