硝酸盐异化还原为铵耦合厌氧氨氧化处理含氮废水

赵硕; 汪超; 杨蒙; 乔森

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中国环境科学 ›› 2024, Vol. 44 ›› Issue (8) : 4389-4399.

水污染与控制

硝酸盐异化还原为铵耦合厌氧氨氧化处理含氮废水

赵硕¹, 汪超¹, 杨蒙², 乔森¹

作者信息 +

Treatment of nitrogen-containing wastewater by dissimilatory nitrate reduction to ammonium coupled with anaerobic ammonium oxidation

ZHAO Shuo¹, WANG Chao¹, YANG Meng², QIAO Sen¹

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文章历史 +

摘要

为探究厌氧氨氧化(Anammox)细菌同时去除废水中铵(NH₄⁺)和硝酸盐(NO₃^-)的可行性及长期去除效果,基于Anammox菌的内源硝酸盐异化还原为铵过程,进行了批式实验、抑制剂实验、同位素实验以及长期反应器实验.结果表明,在MLVSS为4500mg/L的批式实验中,经12h反应后NH₄⁺和NO₃^-被同时去除并生成了N₂,总氮去除率为93.49%.在乙炔(抑制Anammox反应中铵和一氧化氮生成肼的过程)抑制实验中,检测到17.01mg N/L的NH₄⁺,Anammox菌对于NO₃^-去除的贡献率为85.88%.当加入青霉素G(抑制异养反硝化菌活性)后,NO₃^-仍可以被去除.在¹⁴NH₄⁺和¹⁵NO₃^-同位素示踪实验中一直能够检测到²⁹N₂和³⁰N₂的生成,前者是后者含量的1.41倍.在长期实验中,反应器稳定运行阶段平均出水NH₄⁺和NO₃^-浓度分别为1.18和2.86mg N/L,平均总氮去除率为92.26%.由此可见,Anammox菌通过内源硝酸盐异化还原为铵耦合常规Anammox过程,可以长期稳定地同时去除废水中NH₄⁺和NO₃^-.

Abstract

To investigate the feasibility and long-term performance of simultaneous removal of ammonium (NH₄⁺) and nitrate (NO₃^-) in wastewater by anaerobic ammonia oxidation (anammox) bacteria, batch, inhibitor, isotopic and long-term reactor experiments were conducted based on the intracellular carbon-dependent dissimilatory nitrate reduction to ammonium (DNRA) of anammox bacteria. In 12h batch experiments, NH₄⁺ and NO₃^- were simultaneously converted to N₂ with anammox biomass concentration of 4500mg/L, and the total nitrogen removal efficiency was 93.49%. In the experiment of acetylene inhibition (inhibiting the process of generating hydrazine from NH₄⁺and nitric oxide in the anammox reaction), 17.01mg N/L of NH₄⁺ was detected, and the contribution rate of anammox bacteria to NO₃^-removal was 85.88%. After the addition of penicillin G (an inhibitor for most heterotrophic denitrifying bacteria), NO₃^- could still be removed. Both ²⁹N₂ and ³⁰N₂ was always detected from the very beginning in the ¹⁵N isotope tracing experiment, and the former was 1.4 folds higher than the latter. In the long-term experiment, the average NH₄⁺ and NO₃^- concentrations of effluent were 1.18 and 2.86mg N/L respectively, reaching an average total nitrogen removal efficiency of 92.26%. These results suggested that NH₄⁺ and NO₃^-could be simultaneously removed by endogenous DNRA coupled with normal anammox metabolism, further promoting the application of anammox-based processes for nitrogen-contained wastewater removal.

导出引用

赵硕, 汪超, 杨蒙, 乔森. 硝酸盐异化还原为铵耦合厌氧氨氧化处理含氮废水[J]. 中国环境科学. 2024, 44(8): 4389-4399

ZHAO Shuo, WANG Chao, YANG Meng, QIAO Sen. Treatment of nitrogen-containing wastewater by dissimilatory nitrate reduction to ammonium coupled with anaerobic ammonium oxidation[J]. China Environmental Science. 2024, 44(8): 4389-4399

中图分类号： X703

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