聚乙烯对小白菜生长的潜在影响:内生效应

于方明; 高雨; 陈玉橼; 符明月; 唐炽健; 莫翠菊; 李艺

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中国环境科学 ›› 2024, Vol. 44 ›› Issue (8) : 4621-4631.

美丽中国建设:新污染物

聚乙烯对小白菜生长的潜在影响:内生效应

于方明^1,2, 高雨¹, 陈玉橼¹, 符明月¹, 唐炽健¹, 莫翠菊¹, 李艺^1,2

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Potential impact of polyethylene on the growth of pakchoi (Brassica campestris L.): Endophyte effects

YU Fang-ming^1,2, GAO Yu¹, CHEN Yu-yuan¹, FU Ming-yue¹, TANG Chi-jian¹, MO Cui-ju¹, LI Yi^1,2

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摘要

以小白菜作为研究对象,通过盆栽实验,分析了不同粒径和不同添加水平的聚乙烯微塑料(PE-MPs)对小白菜生长及其生理生化特性的潜在影响.研究结果表明,PE添加显著降低了小白菜的株高和株重(P<0.05),在PE粒径为0.5µm,投加量为0.5%(w/w)的处理下,分别下降了86.8%和60.3%.并且,PE添加导致小白菜叶片中光合色素的含量显著降低(P<0.05).二元方差分析结果表明,PE粒径变化对小白菜生长和光合色素含量变化的影响更大.PE添加显著降低了内生细菌群落的Shannon指数和ACE指数(P<0.05),显著提高了内生细菌群落的Simpson指数(P<0.05).在各水平PE的添加下,小白菜根系内生细菌变形菌门和内生真菌子囊菌门的相对丰度升高,而内生细菌放线菌门的相对丰度降低.SEM观测结果表明PE-MPs颗粒主要出现在小白菜的木质部和根皮层组织的细胞壁上.二元方差分析结果表明,PE粒径变化对小白菜根系和地上部分的氧化应激指标的影响更大.PE的富集对小白菜造成了严重的结构、功能损伤和膜脂过氧化损伤,最终导致小白菜的生物量下降.

Abstract

In the current study, pakchoi (Brassica campestris L.) was selected and pot experiments were conducted to investigate the potential impact of polyethylene microplastic (PE-MPs) addition at different size and levels on the growth and physiological and biochemical variations of pakchoi. PE exposure resulted in a significant decrease in height and weight of pakchoi (P<0.05), with decreased by 86.8% and 60.3%, respectively, at PE particle size of 0.5 µm, addition level of 0.5% (w/w). Meanwhile, PE exposure resulted in a significant decrease in photosynethics pigments concentrations in leaves of pakchoi (P<0.05). Two-way ANOVA results indicated that the PE particle size has greater impact on the growth of pakchoi and the variations of photosynethics pigments concentrations. In addtions, PE exposure resulted in a significant decrease in both Shannon and ACE indices for bacteria (P<0.05), and a significant increased of Simpson index for fungi (P<0.05). Moreover, the relative abundance of Proteobacteria and Ascomycota increased, while the relative abundance of Actinobacteriota decreased under different PE additions. SEM images showed that PE-MPs occurred mostly in the xylem and cell walls of the root cortical tissues of pakchoi. Two-way ANOVA results indicated that the PE particle size has greater impact on oxidative stress contents. PE exposure causing serious structural and functional damage and membrane lipid peroxidation damage of pakchoi, and leading to a decrease in pakchoi biomass.

导出引用

于方明, 高雨, 陈玉橼, 符明月, 唐炽健, 莫翠菊, 李艺. 聚乙烯对小白菜生长的潜在影响:内生效应[J]. 中国环境科学. 2024, 44(8): 4621-4631

YU Fang-ming, GAO Yu, CHEN Yu-yuan, FU Ming-yue, TANG Chi-jian, MO Cui-ju, LI Yi. Potential impact of polyethylene on the growth of pakchoi (Brassica campestris L.): Endophyte effects[J]. China Environmental Science. 2024, 44(8): 4621-4631

中图分类号： X172

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