硫氰酸盐胁迫下Anammox颗粒污泥脱氮响应机制

李佩瑶; 周鑫

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (10) : 5500-5508.

水污染与控制

硫氰酸盐胁迫下Anammox颗粒污泥脱氮响应机制

李佩瑶¹, 周鑫^1,2

作者信息 +

Response mechanism of nitrogen removal in Anammox granular sludge under thiocyanate stress

LI Pei-yao¹, ZHOU Xin^1,2

Author information +

文章历史 +

摘要

采用短期批量实验探究了SCN^-(0~500mg/L)添加下对厌氧氨氧化(Anammox)颗粒污泥系统的影响机制.结果表明:随着进水SCN^-浓度的提高,Anammox脱氮效率显著下降,总氮去除率从对照组的88.6%降至43.3%(500mg/L SCN^-);厌氧氨氧化活性(SAA)也呈现出快速下降趋势.非竞争型抑制模型确定了SCN^-对Anammox半抑制浓度(IC₅₀)为209.5mg/L.添加SCN^-后Anammox细胞分泌的EPS增高.PN/PS随着SCN^-增加而上升;SCN^-添加后,Candidatus Brocadia相对丰度明显降低,而Thiobacillus相对丰度急剧增高,微生物功能代谢途径丰度出现不同程度下降.研究结果可为处理含SCN^-的含氮废水Anammox工程应用提供理论基础.

Abstract

In this study, short-term batch experiment was used to investigate the mechanism of thiocyanate (0~500mg/L) addition on the anammox granular sludge system. The results showed that as the concentration of thiocyanate influent increased, nitrogen removal efficiency of Anammox decreased significantly, and the total nitrogen removal efficiency decreased from 88.6% in the control group to 43.3% (500mg/L thiocyanate); The anammox activity (SAA) also showed a rapid decreasing trend. The non-competitive inhibition model determined the 50% inhibitory concentration (IC₅₀) of thiocyanate on Anammox to be 209.5mg/L. The EPS secreted by Anammox cells increased after the addition of thiocyanate. The PN/PS increased with the rise of the concentration of thiocyanate. The relative abundance of Candidatus Brocadia was significantly decreased after the addition of thiocyanate, while the relative abundance of Thiobacillus increased sharply, and The abundance of microbial functional metabolism pathways appeared to be reduced to different degrees. The research results can provide a theoretical basis for the application of Anammox engineering in treating nitrogenous wastewater containing thiocyanate.

导出引用

李佩瑶, 周鑫. 硫氰酸盐胁迫下Anammox颗粒污泥脱氮响应机制[J]. 中国环境科学. 2025, 45(10): 5500-5508

LI Pei-yao, ZHOU Xin. Response mechanism of nitrogen removal in Anammox granular sludge under thiocyanate stress[J]. China Environmental Science. 2025, 45(10): 5500-5508

中图分类号： X703

参考文献

[1] Raper E, Stephenson T, Fisher R, et al. Characterisation of thiocyanate degradation in a mixed culture activated sludge process treating coke wastewater [J]. Bioresource Technology, 2019,288:121524.
[2] Yu X, Nishimura F, Hidaka T. Anammox reactor exposure to thiocyanate: Long-term performance and microbial community dynamics [J]. Bioresource Technology, 2020,317:123960.
[3] 徐伟超,蒙小俊,尹莉,等.焦化废水活性污泥中降解硫氰化物细菌种群多样性分析 [J]. 环境科学, 2016,37(7):2689-2695. Xu W C, Meng X J, Yin L, et al. Analysis of the diversity of bacterial populations degrading thiocyanate in activated sludge from coking wastewater [J]. Environmental Science, 2016,37(7):2689-2695.
[4] Chen Q Q, Sun F Q, Guo Q, et al. Process stability in an anammox UASB reactor with individual and combined thiocyanate and hydraulic shocks [J]. Separation and Purification Technology, 2017, 173:165-173.
[5] Shafiei F, Watts M P, Pajank L, et al. The effect of heavy metals on thiocyanate biodegradation by an autotrophic microbial consortium enriched from mine tailings [J]. Applied Microbiology and Biotechnology, 2020,105(1):417-427.
[6] Wang Q, Wang Y, Lin J, et al. Selection of seeding strategy for fast start-up of Anammox process with low concentration of Anammox sludge inoculum [J]. Bioresource Technology, 2018,268:638-647.
[7] Chen Q Q, Chen H, Zhang Z Z, et al. Effects of thiocyanate on granule-based anammox process and implications for regulation [J]. Journal of Hazardous Materials, 2017,321:81-91.
[8] Chen X, Duan F, Yu X, et al. One-stage anammox and thiocyanate- driven autotrophic denitrification for simultaneous removal of thiocyanate and nitrogen: Pathway and mechanism [J]. Water Research, 2024,265:122268.
[9] Yang Y, Zhang L, Cheng J, et al. Achieve efficient nitrogen removal from real sewage in a plug-flow integrated fixed-film activated sludge (IFAS) reactor via partial nitritation/anammox pathway [J]. Bioresource Technology, 2017,239:294-301.
[10] Miao L, Zhang Q, Wang S, et al. Characterization of EPS compositions and microbial community in an Anammox SBBR system treating landfill leachate [J]. Bioresource Technology, 2018,249:108-116.
[11] Wei Z, Xu R, Li H, et al. Mixotrophic denitrification using thiocyanate and phenol as electron donors: Kinetics and microbial mechanisms [J]. Journal of Water Process Engineering, 2024,61:105260.
[12] Pan J, Ma J, Wu H, et al. Application of metabolic division of labor in simultaneous removal of nitrogen and thiocyanate from wastewater [J]. Water Research, 2019,150:216-224.
[13] Pan J, Ma J, Wu H, et al. Simultaneous removal of thiocyanate and nitrogen from wastewater by autotrophic denitritation process [J]. Bioresource Technology, 2018,267:30-37.
[14] Pan J, Wei C, Fu B, et al. Simultaneous nitrite and ammonium production in an autotrophic partial denitrification and ammonification of wastewaters containing thiocyanate [J]. Bioresource Technology, 2018,252:20-27.
[15] Liu L, Xu Y, Yu C, et al. The efficient utilization of thiocyanate on simultaneous removal of ammonium and nitrate through thiosulfate- driven autotrophic denitrifiers and anammox [J]. Bioresource Technology, 2023,380:129069.
[16] Chen X, Yang L, Sun J, et al. Modelling of simultaneous nitrogen and thiocyanate removal through coupling thiocyanate-based denitrification with anaerobic ammonium oxidation [J]. Environmental Pollution, 2019,253:974-980.
[17] Chen X, Duan F, Yu X, et al. Uncovering pathway and mechanism of simultaneous thiocyanate detoxicity and nitrate removal through anammox and denitrification [J]. NPJ Clean Water, 2024,7(1): DOI:10.1038/s41545-024-00402-w.
[18] Kim Y M, Park D, Lee D S, et al. Inhibitory effects of toxic compounds on nitrification process for cokes wastewater treatment [J]. Journal of Hazardous Materials, 2008,152(3):915-921.
[19] Guo Q, Shi Z J, Yang C C, et al. Individual and combined inhibition of phenol and thiocyanate on microbial activity of partial nitritation [J]. Environmental Science and Pollution Research, 2017,24(16):14207- 14217.
[20] Liu X, Liu J, Deng D, et al. Investigation of extracellular polymeric substances (EPS) in four types of sludge: Factors influencing EPS properties and sludge granulation [J]. Journal of Water Process Engineering, 2021,40:101924.
[21] Wang W, Yan Y, Zhao Y, et al. Characterization of stratified EPS and their role in the initial adhesion of anammox consortia [J]. Water Research, 2020,169:115223.
[22] Yu P, Wang D, Jin X, et al. Rapid formation mechanism of SAD granular sludge: From flocculated activated sludge to granular sludge [J]. Journal of Water Process Engineering, 2024,64:105627.
[23] Zhu L, Zhou J, Lv M, et al. Specific component comparison of extracellular polymeric substances (EPS) in flocs and granular sludge using EEM and SDS-PAGE [J]. Chemosphere, 2015,121:26-32.
[24] He S, Yang W, Qin M, et al. Performance and microbial community of anammox in presence of micro-molecule carbon source [J]. Chemosphere, 2018,205:545-552.
[25] Chen W, Dai X, Cao D, et al. Characterization of a microbial community in an Anammox process using stored anammox sludge [J]. Water, 2017,9:829-840.
[26] He J, Zhang Q, Tan B, et al. Understanding the effect of residual aluminum salt coagulant on activated sludge in sequencing batch reactor: Performance response, activity restoration and microbial community evolution [J]. Environmental Research, 2022,212:113449.
[27] Wang W, Li D, Li S, et al. Insight into enrichment of anaerobic ammonium oxidation bacteria in anammox granulation under decreasing temperature and no strict anaerobic condition: Comparison between continuous and sequencing batch feeding strategies [J]. Science of The Total Environment, 2021,787:147601.
[28] 赵彬,丁雪松,吴丹青,等.高负荷条件下好氧颗粒污泥同步脱氮除碳特性及微生物群落结构分析 [J]. 环境工程学报, 2020,14(2): 295-304. Zhao B, Ding X, Wu D Q, et al. Characteristics on simultaneous nitrogen and organic carbon removal and microbial community structure analysis of aerobic granular sludge treating high strength wastewater [J]. Chinese Journal of Environmental Engineering, 2020, 14(2):295-304.
[29] Hung C H, Wong S S, Cheng P J, et al. Successful enrichment of rarely found Candidatus Anammoxoglobus propionicus from leachate sludge [J]. Journal of Microbiology and Biotechnology, 2014,24(7):879-887.
[30] Kim S J, Katayama Y. Effect of growth conditions on thiocyanate degradation and emission of carbonyl sulfide by Thiobacillus thioparus THI115 [J]. Water Research, 2000,34(11):2887-2894.
[31] Yang Y, Niu Q, Lu J, et al. The inhibitory effects and underlying mechanism of high ammonia stress on sulfide-driven denitrification process [J]. Chemosphere, 2022,303:135093.
[32] Wei Z, Zhang B, Xu R, et al. Mixotrophic denitrification using thiocyanate as an electron donor: Role of thiocyanate under different C/N conditions [J]. Journal of Environmental Management, 2025, 375:124264.
[33] Li X, Yuan Y, Dang P, et al. Effect of salinity stress on nitrogen and sulfur removal performance of short-cut sulfur autotrophic denitrification and anammox coupling system [J]. Science of The Total Environment, 2023,878:162982.
[34] Chen W, Chen S, Hu F, et al. A novel anammox reactor with a nitrogen gas circulation: performance, granule size, activity, and microbial community [J]. Environmental Science and Pollution Research, 2020, 27(15):18661-18671.
[35] Hong X, Niu B, Sun H, et al. Insight into response characteristics and inhibition mechanisms of anammox granular sludge to polyethylene terephthalate microplastics exposure [J]. Bioresource Technology, 2023,385:129355.
[36] Xiao C, Wan K, Hu J, et al. Performance changes in the anammox process under the stress of rare-earth element Ce(III) and the evolution of microbial community and functional genes [J]. Bioresource Technology, 2023,384:129349.