荧蒽对污泥和厨余垃圾厌氧共消化的影响机制

赵银兰; 秦芷怡; 严媛媛; 冯雷雨; 陈银广

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (10) : 5561-5569.

固体废物

荧蒽对污泥和厨余垃圾厌氧共消化的影响机制

赵银兰¹, 秦芷怡¹, 严媛媛², 冯雷雨¹, 陈银广¹

作者信息 +

Influence mechanism of fluoranthene on anaerobic co-digestion of sludge and kitchen waste

ZHAO Yin-lan¹, QIN Zhi-yi¹, YAN Yuan-yuan², FENG Lei-yu¹, CHEN Yin-guang¹

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摘要

在探究荧蒽(Flu)对污泥和厨余垃圾厌氧共消化产甲烷效果的基础上,全面解析了Flu对厌氧共消化的作用机制.在适量浓度范围内(£50mg/kg TSS), Flu对厌氧产甲烷具有促进作用,当Flu为50mg/kg TSS时,共消化系统的甲烷产率达到最高[30.70mL/(gVSS×d)],是空白组的1.61倍.机理研究表明, Flu促进了水解、酸化和乙酸裂解产甲烷过程;提高了厌氧共消化系统中乙酸激酶(ACK)和辅酶F420活性,分别是空白组的1.30和1.43倍.微生物群落结构分析发现Flu不仅促进了与有机物水解和酸化密切相关的普氏菌属和毛球菌属微生物的富集,还提高了乙酸裂解甲烷化的甲烷八叠球菌相对丰度.宏基因组学进一步揭示Flu的存在提高了与糖酵解(HK, pfk和PK)、挥发性脂肪酸产生(por, ackA及buk)和乙酸裂解产甲烷途径(pta, ACSS1_2, cdhD和cdhE)中相关基因丰度,促进产甲烷.

Abstract

On the basis of investigating the effect of fluoranthene (Flu) on the anaerobic co-digestion of sludge and kitchen waste, the mechanism on anaerobic co-digestion was comprehensively analysed. The results demonstrated that anaerobic methanogenesis was enhanced within the appropriate concentration of Flu (£50mg/kg TSS). The maximum methane yield of the anaerobic co-digestion system [30.7mL/(gVSS×d)] was achieved at Flu concentration of 50mg/kg TSS, which was 1.61times that of the control group. Mechanistic studies showed that hydrolysis, acidification and acetoclastic methanogenesis were promoted by Flu, as evidenced by the increased activities of acetate kinase (ACK, 1.30-fold) and coenzyme F420 (1.43-fold) relative to the control. Microbial community structure demonstrated that not only Prevotella and Trichococcus, involved in organic matter hydrolysis and acidification, were selectively enriched in the Flu group, but also the relative abundance of the acetoclastic methanogen Methanosarcina was increased. Metagenomics further revealed that the relative abundances of functional genes associated with glycolysis (HK, pfk and PK), volatile fatty acid production (por, ackA and buk) and acetoclastic methanogenesis (pta, ACSS1_2, cdhD and cdhE) were up-regulated, favouring methanogenesis.

导出引用

赵银兰, 秦芷怡, 严媛媛, 冯雷雨, 陈银广. 荧蒽对污泥和厨余垃圾厌氧共消化的影响机制[J]. 中国环境科学. 2025, 45(10): 5561-5569

ZHAO Yin-lan, QIN Zhi-yi, YAN Yuan-yuan, FENG Lei-yu, CHEN Yin-guang. Influence mechanism of fluoranthene on anaerobic co-digestion of sludge and kitchen waste[J]. China Environmental Science. 2025, 45(10): 5561-5569

中图分类号： X705

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