生物炭对厌氧稻田嘧菌酯降解的影响及其微生物群落变化

陈建均; 马健; 杨欣妍; 和锐敏; 卢颖林; 安玉兴; 李爽

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (10) : 5648-5656.

土壤污染与控制

生物炭对厌氧稻田嘧菌酯降解的影响及其微生物群落变化

陈建均¹, 马健¹, 杨欣妍², 和锐敏², 卢颖林², 安玉兴², 李爽²

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Effects of biochar on the degradation of azoxystrobin in anaerobic paddy field and its microbial community changes

CHEN Jian-jun¹, MA Jian¹, YANG Xin-yan², HE Rui-min², LU Ying-lin², AN Yu-xing², LI Shuang²

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摘要

以华南稻田土壤为研究对象,通过构建微宇宙体系,研究了淹水稻田中添加甘蔗渣生物炭对嘧菌酯在土壤中的降解转化过程及其微生物群落结构组成的影响.结果表明,添加甘蔗渣生物炭(300,500,700℃)在培养前期(10d)能促进嘧菌酯降解(降解率32.42%~34.87%),高于未添加生物炭土壤的16.48%;在培养后期(50d)抑制其最终降解(生物炭处理组降解率为88.78%~89.76%,未添加生物炭处理组为98.77%),呈现先促进后抑制的变化.随着土壤中700℃生物炭(BC 700)的添加量增加,嘧菌酯降解的抑制效果越明显.同时还鉴定出嘧菌酯代谢物嘧菌酯酸.通过16S rRNA高通量分析体系中微生物群落结构组成,结果表明BC700的添加会降低土壤中微生物物种总数和丰富度.5%和10%生物炭添加量与原始土壤、3%生物炭添加量对比,热脱硫杆菌门Desulfobacterota相对丰度降低,Citrifermentans和芽孢杆菌属Bacillus的相对丰度提高,梭状芽孢杆菌属Clostridium_sensu_stricto_12的相对丰度减少.

Abstract

This study investigated the effects of sugarcane bagasse biochar on the degradation of azoxystrobin and the structural composition of microbial communities in flooded paddy soils from South China using a microcosm system. Results demonstrated that biochar pyrolyzed at 300°C, 500°C, and 700°C accelerated azoxystrobin degradation during the early incubation phase (10d), achieving degradation rates of 32.42%~34.87%, significantly higher than the 16.48% observed in the control (no biochar). However, it ultimately inhibited complete degradation by the late phase (50d), with final degradation rates of 88.78%~89.76% in biochar-amended soils versus 98.77% in the control, demonstrating an initial stimulation followed by suppression. The inhibitory effect intensified with increasing application rates of 700°C biochar (BC700). Azoxystrobin acid, a key metabolite, was identified during this process. High-throughput 16S rRNA sequencing revealed that BC700 addition reduced total microbial species richness and Chao1index. Comparative analysis showed that 5% and 10% biochar treatments significantly decreased the relative abundance of Desulfobacterota while increasing Citrifermentans and Bacillus spp. compared to pristine soil and the 3% biochar treatment. Conversely, Clostridium_sensu_stricto_12 abundance declined.

导出引用

陈建均, 马健, 杨欣妍, 和锐敏, 卢颖林, 安玉兴, 李爽. 生物炭对厌氧稻田嘧菌酯降解的影响及其微生物群落变化[J]. 中国环境科学. 2025, 45(10): 5648-5656

CHEN Jian-jun, MA Jian, YANG Xin-yan, HE Rui-min, LU Ying-lin, AN Yu-xing, LI Shuang. Effects of biochar on the degradation of azoxystrobin in anaerobic paddy field and its microbial community changes[J]. China Environmental Science. 2025, 45(10): 5648-5656

中图分类号： X53

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