模拟实验研究城市公园土壤潜在N2O排放对铅添加的响应机制

刘伟; 赵雯淑; 沈玉叶; 蒋文婷; 田琳琳; 王燕; 王敬; 蔡延江

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (10) : 5657-5670.

土壤污染与控制

模拟实验研究城市公园土壤潜在N₂O排放对铅添加的响应机制

刘伟^1,2, 赵雯淑^1,2, 沈玉叶^1,2, 蒋文婷^1,2, 田琳琳^1,3, 王燕^1,2, 王敬⁴, 蔡延江^1,2

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Simulated experiment for investigatingthe response mechanisms of potential N₂O emissions to lead addition from urban park soil

LIU Wei^1,2, ZHAO Wen-shu^1,2, SHEN Yu-ye^1,2, JIANG Wen-ting^1,2, TIAN Lin-lin^1,3, WANG Yan^1,2, WANG Jing⁴, CAI Yan-jiang^1,2

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摘要

以亚热带典型城市公园土壤为研究对象,通过向土壤中添加不同浓度PbCl₂溶液(设置5个处理:Pb添加量分别为CK:不添加Pb的对照,Pb₃₀: 30mg/kgsoil,Pb₆₀: 60mg/kgsoil,Pb₁₂₀: 120mg/kgsoil,Pb₂₄₀: 240mg/kgsoil)后进行28d的室内培养实验,以模拟研究城市公园土壤潜在N₂O排放对不同Pb添加的响应机制.结果表明:Pb添加后28d内Pb₃₀~Pb₂₄₀处理的公园土壤N₂O累积排放量均显著高于CK,各处理土壤N₂O排放量均随外源Pb添加量和土壤Pb含量增加而呈线性增加;Pb添加14d后,城市公园土壤亚硝酸盐还原酶和N₂O还原酶的活性随土壤Pb含量增加分别显著增加和降低,共同导致土壤潜在N₂O排放速率随之显著增加;Pb添加14~28d内,土壤pH值随土壤Pb含量升高而显著降低,土壤pH值下降及Pb含量增加的综合作用对N₂O还原酶活性的抑制效应显著增强;上述过程的共同作用导致培养期内土壤N₂O累积排放量随Pb含量升高而显著增加.本研究表明含Pb污染物在城市公园土壤的累积会增加潜在N₂O排放风险,未来应重视含Pb污染物对城市公园土壤氮循环及其反硝化微生物的影响.本研究可为城市公园管理和土壤N₂O减排措施的制定提供科学指导.

Abstract

In this study, five treatments (CK: control with no Pb input, Pb₃₀:30mg/kg soil, Pb₆₀:60mg/kg soil, Pb₁₂₀:120mg/kg soil, Pb₂₄₀:240mg/kg soil) were set up by adding PbCl₂ solution withdifferent Pb concentrations to atypical subtropical urban park soil. The indoor incubation experiments were carried out for 28d to investigate the response mechanism of potential N₂O emissions from urban park soilto different exogenous Pb additions.The results showed that the cumulative N₂O emissions from Pb₃₀~Pb₂₄₀ treatments were significantly higher than those from CK, which increased linearly with the increases of exogenous Pb additions and soil Pb contents, during the whole incubation period. After 14d of Pb addition, the activities of nitrite reductase and N₂O reductase increased and decreased with the increasing soil Pb content, respectively, which resulted in significant increases in the potential N₂O emission ratesfrom soil.After 14~28d of Pb addition, soil pH significantly decreased with the increasing soil Pb content, and the combined inhibitory effects of decreasing pH and increasing Pb content on the N₂O reductase activity were significantly enhanced. The combined effects of the above-mentionedprocesses ultimately resulted in significant increases in the potential N₂O cumulative emissionsfrom soilwith increasing Pb additions during the incubation period. This study indicates that the effects of Pb pollutants on nitrogen cycle and denitrification microorganisms should be paid more attention in the future,considering the accumulations of Pb pollutants in urban park soil will increase the risk of potential N₂O emission. This study can provide scientific guidance for management of urban park and formulating measures for the reduction of N₂O emissions from urban park soil.

导出引用

刘伟, 赵雯淑, 沈玉叶, 蒋文婷, 田琳琳, 王燕, 王敬, 蔡延江. 模拟实验研究城市公园土壤潜在N₂O排放对铅添加的响应机制[J]. 中国环境科学. 2025, 45(10): 5657-5670

LIU Wei, ZHAO Wen-shu, SHEN Yu-ye, JIANG Wen-ting, TIAN Lin-lin, WANG Yan, WANG Jing, CAI Yan-jiang. Simulated experiment for investigatingthe response mechanisms of potential N₂O emissions to lead addition from urban park soil[J]. China Environmental Science. 2025, 45(10): 5657-5670

中图分类号： X53 X511

参考文献

[1] 刘畅,唐立娜.景感生态学在城市生态系统服务中的应用研究—以城市公园景观设计为例 [J]. 生态学报, 2020,40(22):8141-8146. Liu C, Tang L N. Application of landsenses ecolgy in urban ecosystem services: A case study of urban park landscape design [J]. Acta Ecolgica Sinica, 2020, 40(22):8141-8146.
[2] Li S Z, Ren K X, Yan X, et al. Linking biodiversity and ecolgical function through extensive microeukaryotic movement across different habitats in six urban parks [J]. iMeta, 2023,e103.
[3] CJJ/T85-2017 城市绿地分类标准 [S]. CJJ/T85-2017 Standard for classification of urban green space [S].
[4] 宋洋,贺灿飞,徐阳,等.中国城市公园绿地供需时空格局演化及驱动机制 [J]. 自然资源学报, 2023,38(5):194-1209. Song Y, He C F, Xu Y, et al. Spatio-temporal evolution and driving mechanism of supply and demand of urban park green space in China [J]. Journal of Natural Resources, 2023,38(5):1194-1209.
[5] IPCC. Climate change 2021: The physical science basis. Working Group I contribution to the sixth assessment report of the intergovernmental panel on climate change [R]. UK: Cambridge University Press, 2022.
[6] Zhan Y, Yao Z, Groffman P M, et al. Urbanization can accelerate climate change by increasing soil N₂O emission while reducing CH₄ uptake [J]. Global Change Biology, 2023,29(12):3489-3502.
[7] Allen J A, Setälä H, Kotze D J. Dog urine has acute impacts on soil chemistry in urban greenspaces [J]. Frontiers in Ecology and Evolution, 2020,8:615979 .
[8] Decina S M, Templer P H, Hutyra L R, et al. Variability, drivers, and effects of atmospheric nitrogen inputs across an urban area: Emerging patterns among human activities, the atmosphere, and soils [J]. Science of The Total Environment, 2017,609:1524-1534.
[9] Zhou C C, He Y Q, Gao Z Y, et al. Sex differences in the effects of lead exposure on growth and development in young children [J]. Chemosphere, 2020,250:126294.
[10] 郑袁明,余轲,吴泓涛,等.北京城市公园土壤铅含量及其污染评价 [J]. 地理研究, 2002,21(4):418-424. Zheng Y M, Yu K, Wu H T, et al. Lead concentrations of soils in Beijing urban parks and their pollution assessment [J]. Geographical Research, 2002,21(4):418-424.
[11] 张海珍,唐宇力,陆骏,等.西湖景区土壤典型重金属污染物的来源及空间分布特征 [J]. 环境科学, 2014,35(4):1516-1522. Zhang H Z, Tang Y L, Lu J, et al. Sources and spatial distribution of typical heavy metal pollutants in soils in Xihu Scenic Area [J]. Environmental Science, 2014,35(4):1516-1522.
[12] 杨昱莹,刘亮,陈明,等.长三角地区南京市表土重金属污染特征及源解析 [J]. 中国环境科学, 2024,44(7):3910-3918. Yang Y Y, Liu L, Chen M, et al. Characterization and source analysis of topsoil heavy metal pollution in Nanjing, Yangtze River Delta Region [J]. China Environmental Science, 2024,44(7):3910-3918.
[13] 张娟,王艳春,田宇.北京市属公园土壤重金属分布及风险评价 [J]. 环境科学与技术, 2012,35(6):78-81. Zhang J, Wang Y C, Tian Y. Soil contamination in Beijing's parks and heavy metals risk assessment [J]. Environment Science & Technolgy, 2012,35(6):78-81.
[14] 安江梅朵,张瑞卿,郭广慧,等.北京市城市公园土壤铅累积特征、来源及健康风险 [J]. 环境科学, 2023,44(11):6287-6296. An J M D, Zhang R Q, Guo G H, et al. Accumulation characteristics, sources, and health risks of soil lead of urban parksin Beijing [J]. Environmental Science, 2023,44(11):6287-6296.
[15] 蔡延江,丁维新,项剑.土壤N₂O和NO产生机制研究进展 [J]. 土壤, 2012,44(5):712-718. Cai Y J, Ding W X, Xiang J. Mechanisms of nitrous oxide and nitric oxide production in soils: A review [J]. Soils, 2012,44(5):712-718.
[16] Zhang Q, Chen M, Leng Y F, et al. Organic substitution stimulates ammonia oxidation-driven N₂O emissions by distinctively enriching keystone species of ammonia-oxidizing archaea and bacteria in tropical arable soils [J]. Science of The Total Environment, 2023,872: 162183.
[17] Yang Y J, Liu H X, Lv J L. Response of N₂O emission and denitrification genes to different inorganic and organic amendments [J]. Scientific Reports, 2022,12:3940.
[18] 李彬彬,马军花,武兰芳.土壤溶解性有机物对CO₂和N₂O排放的影响 [J]. 生态学报, 2014,34(16):4690-4697. Li B B, Ma J H, Wu L F. Effects of dissolved organic matter in soil on the emission of CO₂ and N₂O [J]. Acta Ecologica Sinica, 2014,34(16): 4690-4697.
[19] 潘亚男,王娅静,曹文超,等.土壤pH影响氧化亚氮(N₂O)排放的研究进展 [J]. 安徽农学通报, 2017,23(15):19-24,99. Pan Y N, Wang Y J, Cao W C. A review of the influence of Soil pH on nitrous oxide (N₂O) Emission [J]. Anhui Agricultural Science Bulletin, 2017,23(15):19-24,99.
[20] Xiao Z N, Duan C Q, Li S Y, et al. The microbial mechanisms by which long-term heavy metal contamination affects soil organic carbon levels [J]. Chemosphere, 2023,340:139770.
[21] 陈静,刘荣辉,陈岩贽,等.重金属污染对土壤微生物生态的影响 [J]. 生命科学, 2018,30(6):667-672. Chen J, Liu R H, Chen Y Z, et al. Effect of heavy metal pollution on soil microbial ecolgy [J]. Chinese Bulletin of Life Sciences, 2018,30 (6):667-672.
[22] 蔡琼瑶,徐俏,周振,等.外源铅在4种土壤中的老化特征及对土壤化学性质的影响 [J]. 环境科学学报, 2019,39(3):899-907. Cai Q Y, Xu Q, Zhou Z, et al. Aging process of Pb affects the chemical properties of four types of soil [J]. Acta Scientiae Circumstantiae, 2019,39(3):899-907.
[23] 邓青梅,余文静,江文宇,等.不同污染方式下铅对茶园土壤氮素转化及其相关微生物和酶活性的影响 [J]. 土壤, 2023,55(5):1054-1061. Deng Q M, Yu W J, Jang W Y, et al. Effects of lead on nitrogen transformation and related microorganisms and enzyme activities in tea garden soil under different pollution modes [J]. Soils, 2023,55 (5):1054-1061.
[24] Lu L, Chen C, Tan K, et al. Long-term metal pollution shifts microbial functional profiles of nitrification and denitrification in agricultural soils [J]. Science of The Total Environment, 2022,830:154732.
[25] Hu X S, Liu X X, Zhang S, et al. Nitrogen-cycling processes under long-term compound heavy metal(loids) pressure around a gold mine: Stimulation of nitrite reduction [J]. Journal of Environmental Sciences, 2025,147:571-581.
[26] Liu Y, Shen K, Wu Y, et al. Abundance and structure composition of nirK and nosZ genes as well as denitrifying activity in heavy metal- polluted paddy soils [J]. Geomicrobiolgy Journal, 2017,35(2):100- 107.
[27] 陈中云,闵航,张夫道,等.4种重金属污染对稻田土反硝化细菌的影响 [J]. 中国环境科学, 2003,(3):82-87. Chen Z Y, Min H, Zhang F D, et al. Effect of four kinds of heavy metal contamination on denitrifying bacteria in paddy soils [J]. China Environmental Science, 2003,(3):82-87.
[28] Vásquez-Murrieta M S, Mondragón C C, Tapia N T, et al. Nitrous oxide production of heavy metal contaminated soil [J]. Soil Biology and Biochemistry, 2006,38(5):931-940.
[29] GB15618-1995 土壤环境质量标准 [S]. GB15618-1995 Environmental quality standard for soils [S].
[30] Jiang W T, Zhang H K, Fang Y Y, et al. Understory N application overestimates the effect of atmospheric N deposition on soil N₂O emissions [J]. Geoderma, 2023,437:116611.
[31] Domeignoz-Horta L A, Philippot L, Peyrard C, et al. Peaks of in situ N₂O emissions are influenced by N₂O-producing and reducing microbial communities across arable soils [J]. Global Change Biology, 2018,24(1):360-370.
[32] 鲁如坤.土壤农业化学分析方法 [M]. 北京:中国农业科技出版社, 2000:12-163. Lu R K. Analytical methods for soil and agro-chemistry [M]. Beijing: China Agricultural Science and Technolgy Press, 2000:12-163.
[33] Zhong W H, Bian B Y, Gao N, et al. Nitrogen fertilization induced changes in ammonia oxidation are attributable mostly to bacteria rather than archaea in greenhouse-based high N input vegetable soil [J]. Soil Biology and Biochemistry, 2016,93:150–159.
[34] Throbäck I N, Enwall K, Jarvis, et al. Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE [J]. FEMS Microbiolgy Ecology, 2004,49(3):401-417.
[35] Li H L, Zhang Y, Wang T T, et al. Responses of soil denitrifying bacterial communities carrying nirS, nirK, and nosZ genes to revegetation of moving sand dunes [J]. Ecological Indicators, 2019, 107:105541.
[36] Henry S, Bru D, Stres B, et al. Quantitative detection of the nosZ gene, encoding nitrous oxide reductase, and comparison of the abundances of 16S rRNA, narG, nirK, and nosZ genes in soils [J]. Applied and Environmental Microbiolgy, 2006,72(8):5181-5189.
[37] 蒋文婷,田立斌,朱高荻,等.不同形态氮添加对毛竹林土壤N₂O排放的影响 [J]. 植物营养与肥料学报, 2022,28(5):857-868. Jiang W T, Tian L B, Zhu G D, et al. Effects of different forms of nitrogen addition on N₂O emissions from the soil of Moso bamboo (Phyllostachys edulis) forest [J]. Journal of Plant Nutrition and Fertilizers, 2022,28(5):857-868.
[38] 吴晓芬,陈有超,蒋文婷,等.模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响 [J]. 草地学报, 2024,23(9):2794-2802. Wu X F, Chen Y C, Jiang W T, et al. The response of potential N₂O emission from an alpine meadow soil to simulated livestock grazing [J]. Acta Agrestia Sinica, 2024,32(9):2794-2802.
[39] 赵迪.重金属胁迫对潮滩沉积物反硝化作用影响机制的初步研究 [D]. 上海:华东师范大学, 2013. Zhao D. A preliminary research on the effect of heavy metals on denitrification in tidal flat sediments [D]. Shanghai: East China Normal University, 2013.
[40] 胡荣桂,李玉林,彭佩钦,等.重金属镉、铅对土壤生化活性影响的初步研究 [J]. 农业环境科学学报, 1990,(4):6-9. Hu R G, Li Y L, Peng P Q, et al. A preliminary study on the effects of cadmium and lead on soil biochemical activity [J]. Journal of Agro-Environment Science, 1990,(4):6-9.
[41] Gillette K L, Qian Y L, Follett R F, et al. Nitrous oxide emissions from a golf course fairway and rough after application of different nitrogen fertilizers [J]. Journal of Environmental Quality, 2016,45(5):1788- 1795.
[42] Ruser R, Schulz R. The effect of nitrification inhibitors on the nitrous oxide (N₂O) release from agricultural soils—a review [J]. Journal of Plant Nutrition and Soil Science, 2015,178(2):171-188.
[43] 冯琪.厌氧条件下初始NO₃^-含量对土壤反硝化气体(N₂、N₂O和NO)和CO₂排放的影响 [D]. 南京:南京农业大学, 2011. Feng Q. Influence of nitrate concentrations on the production of N₂, N₂O, NO, and CO₂ by soil cores during anaerobic incubation [D]. Nanjing:Nanjing Agricultural University, 2011.
[44] 刘兴,李连星,薄香兰,等.铜绿假单胞菌YY24的异养硝化—好氧反硝化功能基因的研究 [J]. 水产科学, 2018,37(4):475-483. Liu X, Li L X, Bo L X.Study on heterotrophic nitrification-aerobic denitrification functional gene of Pseudomonas Aeromonas YY24 [J]. Fisheries Science, 2018,37(4):475-483.
[45] Juwarkar A A, Nair A, Dubey K V, et al.Biosurfactant technolgy for remediation of cadmium and lead contaminated soils [J]. Chemosphere, 2007,68(10):1996-2002.
[46] 吴海江,茆灿泉,郭红光.微生物与重金属作用机理研究 [J]. 安徽农业科学, 2009,37(11):5068-5071. Wu H J, Mao C Q, Guo H G. Study on the interaction between microorganisms and heavy metals [J]. Journal of Anhui Agricultural Sciences, 2009,37(11):5068-5071.
[47] 王泽煌,王蒙,蔡昆争,等.细菌对重金属吸附和解毒机制的研究进展 [J]. 生物技术通报, 2016,32(12):13-18. Wang Z H, Wang M, Cai K Z, et al. Research advances on biosorption and detoxification mechanisms of heavy metals by bacteria [J]. Biotechnolgy Bulletin, 2016,32(12):13-18.
[48] 汪吉东,许仙菊,宁运旺,等.土壤加速酸化的主要农业驱动因素研究进展 [J]. 土壤, 2015,47(4):627-633. Wang J D, Xu X J, Ning Y W, et al. Progresses in agricultural driving factors on accelerated acidification of soils [J]. Soils, 2015,47(4): 627-633.
[49] 翁国华,张居念,张丹,等.外源Pb对不同水稻品种籽粒中Pb含量的基因型差异研究 [J]. 农业环境科学学报, 2009,28(11):2232-2235. Weng G H, Zhang J N, Zhang D, et al. Effect of exogenous lead on genotypic difference of lead content in grains of different rice varieties [J]. Journal of Agro-Environment Science, 2009,28(11):2232-2235.
[50] Bollag J M, Barabasz W. Effect of heavy metals on the denitrification process in soil [J]. Journal of Environmental Quality, 1979,8(2):196- 201.
[51] Kicińska A, Pomykała R, & Izquierdo-Diaz M. Changes in soil pH and mobility of heavy metals in contaminated soils [J]. European Journal of Soil Science, 2022,73(1):e13203.
[52] Chen Y X, Wang K X, Lin Q, et al. Effects of heavy metals on ammonification, nitrification and denitrification in maize rhizosphere [J]. Pedosphere, 2001,11(2):115-122.
[53] Xiao Z N, Duan C Q, Li S Y, et al. The microbial mechanisms by which long-term heavy metal contamination affects soil organic carbon levels [J]. Chemosphere, 2023,340:139770.
[54] Liang S, Guan D X, Ren J H, et al. Effect of aging on arsenic and lead fractionation and availability in soils: Coupling sequential extractions with diffusive gradients in thin-films technique [J]. Journal of Hazardous Materials, 2014,273:272-279.
[55] 王代长,孙志成,蒋新,等.酸性条件下可变电荷土壤表面Pb²⁺-H⁺反应动力学特征 [J]. 环境化学, 2009,28(2):168-172. Wang D Z, Sun Z C, Jiang X, et al. Characteristics of kinetics of Pb²⁺-H⁺ adsorption by selected variable charge soils under acid conditions [J]. Environmental Chemistry, 2009,28(2):168-172.
[56] Holtan-Hartwig L, Bechmann M, Høyås T R, et al. Heavy metals tolerance of soil denitrifying communities: N₂O dynamics [J]. Soil Biology and Biochemistry, 2002,34(8):1181-1190.
[57] Shaaban M, Wu Y P, Khalid M S, et al. Reduction in soil N₂O emissions by pH manipulation and enhanced nosZ gene transcription under different water regimes [J]. Environmental Pollution, 2018,235: 625-631.
[58] Zhang H K, Fang Y Y, Chen Y C, et al. Enhanced soil potential N₂O emissions by land-use change are linked to AOB-amoA and nirK gene abundances and denitrifying enzyme activity in subtropics [J]. Science of The Total Environment, 2022,850:158032.
[59] 曹巧滢,江家泉,王学江,等.新型碱性肥料治酸改土降镉的效果和机理 [J]. 土壤学报, 2023,60(1):175-188. Cao Q Y, Jiang J Q, Wang X J, et al. A novel alkaline fertilizer and its function as well as mechanism to remediation soil acid and Cd pollution [J]. Acta Pedologica Sinica, 2023,60(1):175-188.
[60] 朱津宏,熊若男,杨思琪,等.接种氧化亚氮(N₂O)还原细菌YSQ030对复垦土壤N₂O排放和氮循环关键功能基因的影响 [J]. 南京信息工程大学学报, 2024,16(3):416-427. Zhu J H, Xiong R N, Yang S Q, et al. Effects of inoculation with N₂O reducing bacteria YSQ030 on soil N₂O emission and key functional genes involved innitrogen cycling in reclaimed soil [J]. Journal of Nanjing University of InformationScience & Technology, 2024,16(3): 416-427.