解磷复合菌剂对尾矿的活化作用及其促生提质效果

王晔; 张瑶; 刘子琪; 潘晓静; 郭傲; 于春欣; 段留生

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (11) : 6389-6397.

环境微生物

解磷复合菌剂对尾矿的活化作用及其促生提质效果

王晔, 张瑶, 刘子琪, 潘晓静, 郭傲, 于春欣, 段留生

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Activation effect of phosphorus-solubilizing compound bacterial agents on tailings and its induced growth promotion and quality improvement

WANG Ye, ZHANG Yao, LIU Zi-qi, PAN Xiao-jing, GUO Ao, YU Chun-xin, DUAN Liu-sheng

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摘要

收集16份商业化菌源样品,以有效磷含量为指标,通过初筛不同菌源解磷效果、优化接种量和复配菌种形成复合菌系,并进一步通过盆栽试验,评价尾矿配施复合菌系对小白菜生长和品质的影响.结果表明:筛选获得了4个高效解磷菌及适宜接种量,通过正交试验获得3个复合菌剂(编号为C3、C4和C6),在培养第7d时,其解磷效果较对照提高了7.1~7.6倍.盆栽试验显示,磷尾矿和复合菌剂C4配施处理的小白菜长势较好,与对照相比,叶绿素含量提高了15.96%,产量提高了40.78%,且显著提高了小白菜可溶性蛋白和维生素C含量,分别较对照增加了18.52%和229.36%.综上所述,复合菌剂显著提高了磷尾矿中有效磷含量,对磷尾矿的无害化处理及高效利用具有重要的理论意义和广阔的应用潜力.

Abstract

In this study, 16commercial microbial samples were collected. Leveraging available phosphorus content as the screening criterion, we conducted preliminary screenings of phosphate-solubilizing efficiency in different microbial sources, optimized inoculation quantities, and developed composite microbial consortia through strain combination. Pot experiments were then carried out to evaluate the effects of tailings with composite microbial consortia on the growth and quality of Chinese cabbage. The results show that four efficient phosphorus-solubilizing bacteria were identified after delicately screening and then three composite bacterial agents (numbered as C3, C4 and C6) were obtained by orthogonal experiments. After cultivating for 7d, the phosphate-solubilizing efficiencies of these inoculants were increased by 7.1~7.6 times compared with that of comparison group. The pot experiment indicated that with phosphate tailings and composite microbial inoculant C4 were significantly improved in comparison group, that is the chlorophyll content and yield increased by 15.96% and 40.78%, respectively. Moreover, the contents of soluble protein and vitamin C in Chinese cabbage were significantly increased by 18.52% and 229.36%, respectively. In summary, the composite microbial inoculants significantly increased the available phosphorus content in phosphate tailings and demonstrated an important theoretical significance and a broad application potential in managing harmless treatment and efficient utilization of phosphate tailings.

导出引用

王晔, 张瑶, 刘子琪, 潘晓静, 郭傲, 于春欣, 段留生. 解磷复合菌剂对尾矿的活化作用及其促生提质效果[J]. 中国环境科学. 2025, 45(11): 6389-6397

WANG Ye, ZHANG Yao, LIU Zi-qi, PAN Xiao-jing, GUO Ao, YU Chun-xin, DUAN Liu-sheng. Activation effect of phosphorus-solubilizing compound bacterial agents on tailings and its induced growth promotion and quality improvement[J]. China Environmental Science. 2025, 45(11): 6389-6397

中图分类号： X172 X753

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