PE-MPs与高效氯氟氰菊酯污染对农田土壤细菌群落的影响研究

于皓; 黄力; 吴思瑶; 李建春; 王国洋; 汤家喜; 金德才

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (12) : 6762-6770.

土壤污染与控制

PE-MPs与高效氯氟氰菊酯污染对农田土壤细菌群落的影响研究

于皓¹, 黄力¹, 吴思瑶¹, 李建春¹, 王国洋¹, 汤家喜¹, 金德才²

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Effects of PE-MPs and lambda-cyhalothrin on bacterial community in farmland soil

YU Hao¹, HUANG Li¹, WU Si-yao¹, LI Jian-chun¹, WANG Guo-yang¹, TANG Jia-xi¹, JIN De-cai²

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摘要

选用聚乙烯微塑料(PE-MPs)与高效氯氟氰菊酯进行小白菜盆栽实验,采用Illumina NovaSeq高通量测序技术,对实验第1d和第30d的土壤细菌群落进行测序分析.结果表明,PE-MPs显著抑制小白菜根长、鲜重和株高(P<0.05),与对照相比,分别降低了39.63%、32.9%和19.54%.而高效氯氟氰菊酯则促进其生长,两者复合时高效氯氟氰菊酯可缓解PE-MPs的抑制作用.在实验第1d,高效氯氟氰菊酯与PE-MPs污染会明显影响细菌群落多样性(P<0.05),抑制一些细菌类群的生长.随着实验时间的增加,高效氯氟氰菊酯能缓解由PE-MPs污染对细菌类群产生的抑制作用.LEfSe分析发现不同时间下不同处理组的指示细菌类群均不相同.FAPROTAX功能预测分析发现,与对照组相比,在实验第1d和第30d,高效氯氟氰菊酯可能会抑制碳氢化合物降解功能类群丰度,PE-MPs可能会显著促进其丰度,而复合处理时促进作用可能会减弱.高效氯氟氰菊酯可能会显著增加人类病原体相关功能类群丰度,尤其在实验初期更为明显.对丰度排名前30的属进行互作网络分析表明,PE-MPs在实验末期(第30d)促进丰度前30属的共生协作关系,而复合污染在实验初期(第1d)显著增强此类关系.

Abstract

Pakchoi pot experiment was carried out with polyethylene microplastics (PE-MPs) and lambda-cyhalothrin. The Illumina NovaSeq high-throughput sequencing technology was employed to sequence and analyze the soil bacterial communities on the 1st and 30th days of the experiment. The results demonstrated that PE-MPs significantly inhibited the root length, fresh weight, and plant height of Pakchoi (P<0.05), which were reduced by 39.63%, 32.9%, and 19.54%, respectively, compared to the control. In contrast, lambda-cyhalothrin promoted plant growth, and when applied in combination, it alleviated the inhibitory effects of PE-MPs. On the 1st day of the experiment, both lambda-cyhalothrin and PE-MPs significantly affected the diversity of the bacterial community (P<0.05) and inhibited the growth of some bacterial groups. As the experiment progressed, lambda-cyhalothrin mitigated the inhibitory effect of PE-MPs pollutants on bacterial groups. LEfSe analysis revealed that the indicator bacterial taxa in different treatment groups varied at different times. FAPROTAX functional prediction analysis indicated that, compared to the control, lambda-cyhalothrin may inhibit the abundance of functional groups related to hydrocarbon degradation on both day 1and day 30, while PE-MPs may significantly promote their abundance. However, this promotional effect was attenuated under combined treatment. Lambda-cyhalothrin may significantly increase the abundance of human pathogen-related functional groups, especially at the beginning of the experiment. Interaction network analysis of the top 30 genera in terms of abundance indicated that PE-MPs promoted the symbiotic and cooperative relationships among these genera in terms of abundance at the end of the experiment (day 30), while the combined pollution significantly enhanced such relationships at the beginning of the experiment (day 1). These findings provide a scientific basis for a deeper understanding of the impacts of PE-MPs and lambda-cyhalothrin pollution on the microecological environment of farmland soil.

导出引用

于皓, 黄力, 吴思瑶, 李建春, 王国洋, 汤家喜, 金德才. PE-MPs与高效氯氟氰菊酯污染对农田土壤细菌群落的影响研究[J]. 中国环境科学. 2025, 45(12): 6762-6770

YU Hao, HUANG Li, WU Si-yao, LI Jian-chun, WANG Guo-yang, TANG Jia-xi, JIN De-cai. Effects of PE-MPs and lambda-cyhalothrin on bacterial community in farmland soil[J]. China Environmental Science. 2025, 45(12): 6762-6770

中图分类号： X53

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