巨大芽孢杆菌-青葙组合对镉污染土壤微生物群落演替的驱动作用

赖才星; 林华; 刘泽蕙; 董梓涵; 刘杰; 俞果

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (2) : 1036-1044.

环境微生物

巨大芽孢杆菌-青葙组合对镉污染土壤微生物群落演替的驱动作用

赖才星¹, 林华^1,2,3, 刘泽蕙¹, 董梓涵¹, 刘杰¹, 俞果^1,4

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The driving effect of Bacillus megaterium-Celosia argentea L. combination on the succession of microbial communities in cadmium-contaminated soil

LAI Cai-xing¹, LIN Hua^1,2,3, LIU Ze-hui¹, DONG Zi-han¹, LIU Jie¹, YU Guo^1,4

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摘要

以巨大芽孢杆菌(Bacillus megaterium)和青葙(Celosia argentea L.)的植物-微生物联合修复体系为研究对象,探究了巨大芽孢杆菌对青葙根际微生物群落演替的影响及其在镉污染土壤修复中的作用.通过高通量测序分析了青葙根际微生物在不同时间节点(第7,21,50d)的群落结构变化.结果表明,添加巨大芽孢杆菌的处理组在第50d时,微生物群落的OTUs数量、多样性指数(Shannon、Simpson)和丰富度指数(Chao1、Ace)均高于对照组;酸杆菌门、绿弯菌门、变形菌门和拟杆菌门是微生物群落中的核心菌群;巨大芽孢杆菌能在初期占据根际微生物群落中的主导地位,但其相对丰度在50d内由12.01%逐渐下降至1.17%;土壤微生物群落功能预测显示巨大芽孢杆菌主要促进了土壤中微生物群落的碳循环和氮循环,对微生物群落的功能和稳定性产生了积极的影响;巨大芽孢杆菌能显著增加青葙叶片中的镉含量和根际土壤中的有效态镉含量,增幅分别为40.3%和17.6%.本研究为优化植物-微生物联合修复技术,了解植物-微生物联合修复体系的根际微生物群落演替规律提供了理论基础和实验数据支持.

Abstract

This study focused on the plant-microbe combined remediation system involving Bacillus megaterium and Celosia argentea L., to explore the impact of Bacillus megaterium on the succession of the rhizosphere microbial community and its role in the remediation of cadmium-contaminated soil. High-throughput sequencing analysis was conducted to examine the structural changes in the rhizosphere microbiota of Celosia argentea at different time points (7th, 21st, and 50th days). The results indicated that in the treatment group, inoculated with B. megaterium, the number of OTUs, diversity indices (Shannon, Simpson), and richness indices (Chao1, Ace) of the microbial community were all higher than those in the control group by the 50th day; the Acidobacteriota, Chloroflexi, Proteobacteria, and Bacteroidetes were the core groups within the microbial community; B. megaterium was able to dominate the rhizosphere microbial community in the early stages but its relative abundance gradually declined from 12.01% to 1.17% over the 50days; Functional prediction of the soil microbial community showed that B. megaterium mainly promoted the C and N cycle within the microbial community, potentially exerting a positive influence on the functionality and stability of the microbial community; B. megaterium significantly increased the cadmium content in the leaves of C. argentea and the bioavailable cadmium content in the rhizosphere soil by 40.3% and 17.6%, respectively. This study provides a theoretical foundation and empirical data support for optimizing plant-microbe combined remediation techniques and understanding the succession patterns of microbial communities in plant-microbe combined remediation systems.

导出引用

赖才星, 林华, 刘泽蕙, 董梓涵, 刘杰, 俞果. 巨大芽孢杆菌-青葙组合对镉污染土壤微生物群落演替的驱动作用[J]. 中国环境科学. 2025, 45(2): 1036-1044

LAI Cai-xing, LIN Hua, LIU Ze-hui, DONG Zi-han, LIU Jie, YU Guo. The driving effect of Bacillus megaterium-Celosia argentea L. combination on the succession of microbial communities in cadmium-contaminated soil[J]. China Environmental Science. 2025, 45(2): 1036-1044

中图分类号： X173

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