基于缺氧时间调控内源性亚硝酸盐强化N-EPD-CANON工艺脱氮研究

李冬; 陈晓义; 傅思博; 张景昭; 张杰

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (5) : 2470-2480.

水污染与控制

基于缺氧时间调控内源性亚硝酸盐强化N-EPD-CANON工艺脱氮研究

李冬¹, 陈晓义¹, 傅思博¹, 张景昭¹, 张杰^1,2

作者信息 +

Enhanced nitrogen removal in the N-EPD-CANON process by regulating endogenous nitrite accumulation based on anoxic time

LI Dong¹, CHEN Xiao-yi¹, FU Si-bo¹, ZHANG Jing-zhao¹, ZHANG Jie^1,2

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摘要

采用SBR反应器,以模拟市政污水为进水基质,启动硝化-内源部分反硝化-全程自养脱氮(N-EPD-CANON)工艺.实验通过调控EPD单元缺氧时间考察了单元内亚硝酸盐积累量变化对CANON工艺脱氮性能,功能菌活性以及微生物群落结构的影响.结果表明:在缺氧时间为40min时,EPD单元可以有效地捕获进水有机物,同时维持有利于CANON稳定运行的适量浓度的内源性亚硝酸盐.长期稳定运行下CANON反应器的总氮去除率达到86.43%,比厌氧氨氧化活性(SAA)达到0.82gN/(gVSS·d).此外,EPS质量分数逐渐增长到69.17mg/gVSS,再通过3D-EEM结合PARAFAC技术对污泥的EPS荧光组分进行分析表明,内源性亚硝酸盐的强化作用能够在不改变EPS组成成分的前提下,有效提升了芳香族蛋白质的含量,有助于污泥颗粒化及其稳定性的提高.微生物群落分析揭示,在EPD系统中CandidatusCompetibacter为优势菌属占24.61%,CANON系统中Nitrosomonas的相对丰度为2.67%,这保证了AnAOB菌的NO₂^--N供应.此外,AnAOB的主要组成部分CandidatusBrocadia菌属的相对丰度也达到了13.34%.

Abstract

Employed a SBR to simulate municipal wastewater as the influent matrix and initiated the Nitritation-Enhanced Partial Denitrification-Complete Autotrophic Nitrogen Removal Over Nitrite (N-EPD-CANON) process. The anoxic duration within the EPD system was meticulously adjusted to scrutinize the impact on endogenous nitrite accumulation and the consequent performance alterations within the CANON system. The objective was to elucidate the influence of anoxic time on endogenous nitrite concentration and its subsequent effects on nitrogen removal efficiency, the activity of functional microbial groups, and the structure of microbial communities within the CANON process. The findings revealed that an anoxic duration of 40minutes within the EPD system was optimal for capturing influent organic matter while concurrently promoting the endogenous nitrite to accumulate at a favorable concentration of approximately 4mg/L. Under sustained operational conditions, the CANON reactor achieved a total nitrogen removal rate of 86.43%. The specific anammox activity (SAA) was determined to be 0.82gN/(gVSS·d), the particular nitrate production rate (SNPR) was reduced to 0.28gN/(gVSS·d), and the specific ammonium removal rate (SAOR) was recorded at 0.70gN/(g VSS·d). Additionally, the application of 3D-EEM and PARAFAC techniques to analyze the fluorescence components of EPS in the sludge indicated that the intensification of endogenous nitrite had a beneficial effect on increasing the content of aromatic proteins within the EPS without altering its composition. Microbiota community analysis reveals that Candidatus_Competibacter is the dominant genus in the EPD system, accounting for 24.61%. In contrast, in the CANON system, the relative abundance of Nitrosomonas at 2.67% ensures the NO₂^--N supply for AnAOB, and Candidatus Brocadia, as the main genus of AnAOB, accounts for 13.34%.

导出引用

李冬, 陈晓义, 傅思博, 张景昭, 张杰. 基于缺氧时间调控内源性亚硝酸盐强化N-EPD-CANON工艺脱氮研究[J]. 中国环境科学. 2025, 45(5): 2470-2480

LI Dong, CHEN Xiao-yi, FU Si-bo, ZHANG Jing-zhao, ZHANG Jie. Enhanced nitrogen removal in the N-EPD-CANON process by regulating endogenous nitrite accumulation based on anoxic time[J]. China Environmental Science. 2025, 45(5): 2470-2480

中图分类号： X703.5

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