对比研究了3种不同进水策略对CANON污泥EPS中蛋白质的影响,旨在探求变量进水策略对CANON污泥滞留能力的影响.R1采用常规进水策略作为对照组,而R2和R3采用不同的变量进水策略,分别启动CANON工艺.经过90d的运行后,R1,R2和R3反应器中SVI分别达到了62.93,53.10和57.59mL/g,这说明变量进水的策略可以改善污泥的沉降性能.R2和R3的PN/PS分别达到8.21和7.61,明显高于R1的5.56;三维荧光数据显示,R2的TB-EPS中芳香族蛋白占比达到32.47%,显著高于R1的11.58%和R3的10.5%;这表明变量进水策略会促进TB-EPS中芳香族蛋白的生成,进而提高污泥的疏水性,改善污泥沉降性能.比厌氧氨氧化活性(SAA)分别上升至4.03,4.68和4.36mgN/(gVSS·h),其中R2和R3污泥的SAA达到甚至超过了接种前厌氧氨氧化污泥的SAA,这说明反应器中已经形成了成熟的CANON颗粒污泥.虽然在反应初期R2和R3的微生物活性受到负荷波动的抑制,但随着时间的推移,微生物群体适应了环境变化,恢复了稳定的活性,分别在第54d和第51d实现了CANON工艺的启动.最终在第90d时,R1,R2和R3的总氮去除率分别达到了72.54%,70.14%和73.75%左右,实现了良好的脱氮性能.
Abstract
The effects of three different influent strategies on the protein content of extracellular polymeric substances (EPS) were investigated to assess their impact on sludge retention capacity of the CANON process. R1was operated under conventional influent strategy as the control group, while R2 and R3 were subjected to different variable influent strategies. After 90 days of operation, the SVI values in R1, R2, and R3 were 62.93, 53.10, and 57.59mL/g respectively indicating that the variable influent strategies could improve sludge settleability. The PN/PS ratios were significantly higher in R2 (8.21) and R3 (7.61) compared to R1 (5.56). Three-dimensional fluorescence analysis revealed that the proportion of aromatic proteins in TB-EPS was highest in R2 (32.47%), much higher than in R1 (11.58%) and R3 (10.5%). This indicated that the variable influent strategy promoted the formation of aromatic proteins in TB-EPS, enhancing the hydrophobicity of the sludge and improving settleability. The specific anaerobic ammonium oxidation activity (SAA) increased to 4.03, 4.68 and 4.36mg N/ (g VSS· h) in R1, R2, and R3, respectively. Notably, the SAA in R2 and R3 exceeded that of the seed sludge, indicating the successful formation of mature CANON granular sludge. Although microbial activity in R2 and R3 was initially inhibited by loading fluctuations during early operation, the microbial communities gradually adapted to environmental fluctuations and regained stable metabolic activity, successfully operating the CANON process on day 54 and day 51, respectively. Total nitrogen removal efficiencies reached approximately 72.54%, 70.14%, and 73.75% in R1, R2 and R3 by day 90, demonstrating effective nitrogen removal performance.
关键词
CANON /
变量进水 /
蛋白质组成 /
间歇曝气 /
颗粒污泥
Key words
CANON /
composition of protein /
granular sludge /
intermittent aeration /
variable influent
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基金
北京高校卓越青年科学家计划项目(BJJWZYJH01201910005019)
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