刺槐间作超富集植物修复镉砷复合污染土壤潜力研究

李昊; 雷灿; 王德正; 佘迪凡; 曾鹏; 杜博研; 周航; 辜娇峰; 廖柏寒

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (5) : 2620-2630.

土壤污染与控制

刺槐间作超富集植物修复镉砷复合污染土壤潜力研究

李昊, 雷灿, 王德正, 佘迪凡, 曾鹏, 杜博研, 周航, 辜娇峰, 廖柏寒

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Phytoremediation potential of Cd and As co-contaminated soil by Robinia pseudoacacia L. intercropped with hyperaccumulators

LI Hao, LEI Can, WANG De-zheng, SHE Di-fan, ZENG Peng, DU Bo-yan, ZHOU Hang, GU Jiao-feng, LIAO Bo-han

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摘要

通过盆栽试验,研究刺槐间作两种不同类型的超富集植物龙葵和蜈蚣草对镉(Cd)和砷(As)复合污染土壤的修复效果.结果表明,间作刺槐可促进超富集植物龙葵和蜈蚣草的生长和Cd和As的吸收,降低土壤中Cd和As有效态含量,并提高土壤酶活性.刺槐-龙葵-蜈蚣草间作处理下,龙葵和蜈蚣草的整株生物量较其单作分别显著(P<0.05)提高50.4%和86.2%,同时龙葵叶部Cd和As含量分别显著(P<0.05)提高78.4%和260.7%.刺槐间作龙葵和蜈蚣草处理下所有植物地上部分As累积总量较刺槐和龙葵单作分别显著(P<0.05)提高1.11倍和2.17倍,Cd累积总量较刺槐和蜈蚣草单作分别显著(P<0.05)提高1.89倍和15.72倍,土壤有效态Cd和As含量较对照分别显著(P<0.05)降低23.6%和17.0%.同时,土壤有机质和碱解氮含量较刺槐、龙葵和蜈蚣草单作明显(P<0.05)提高46.2%~83.2%和18.5%~26.4%,土壤过氧化氢酶活性较刺槐和蜈蚣草单作分别显著(P<0.05)提高43.7%~53.0%,蔗糖酶和脲酶活性较刺槐和龙葵单作分别显著(P<0.05)提高11.5%~28.4%和20.6%~36.4%.上述研究表明,刺槐间作两种不同类型的超富集植物可有效吸收和富集污染土壤中的Cd和As来降低土壤中Cd和As的生物有效性,同时有效改善土壤环境质量,可作为一种具有前景的间作模式应用于矿区Cd和As污染土壤的同步修复

Abstract

The remediation effect of Robinia pseudoacacia L. intercropped with Solanum nigrum L. and Pteris vittate L. on Cd and As contaminated soil was studied through a pot experiment. The results showed that the intercropping of R. pseudoacacia L. could promote the growth and the uptake of Cd and As in S. nigrum L. and P. vittate L., and reduce the content of Cd and As in soil, as well as enhance soil enzyme activities. Compared with the monocultures of S. nigrum L. and P. vittate L., the whole biomass of S. nigrum L. and P. vittate L. was significantly enhanced (P<0.05) by 50.4% and 86.2% when intercropped with R. pseudoacacia L. Meanwhile, the contents of Cd and As in the leaves of S. nigrum L. were significantly enhanced (P<0.05) by 78.4% and 260.7%, respectively. The total accumulation of As in aboveground parts of all plants under the intercropping of R. pseudoacacia L. with S. nigrum L. and P. vittate L. was significantly enhanced (P<0.05) by 1.11 and 2.17 times compared with the monocultures of R. pseudoacacia L. or S. nigrum L., and the total accumulation of Cd was significantly enhanced (P<0.05) by 1.89 and 15.72 times compared with the monocultures of R. pseudoacacia L. or P. vittate L. Moreover, the contents of available Cd and As in soil under the intercropping of R. pseudoacacia L. with two hyperaccumulators were significantly reduced (P<0.05) by 23.6% and 17.0% compared with the control, respectively. Meanwhile, the contents of soil organic matter and alkaline hydrolysis nitrogen were significantly enhanced (P<0.05) by 46.2%~83.2% and 18.5%~26.4% as compared with the monocultures, the activities of soil catalase was significantly enhanced (P<0.05) by 43.7%~53.0% compared with the monocultures of R. pseudoacacia L. or P. vittate L., the soil sucrase and urease activities were also significantly enhanced (P<0.05) by 11.5%~28.4% and 20.6%~36.4% compared with the monocultures of R. pseudoacacia L. and S. nigrum L., respectively. The results suggested that the intercropping of R. pseudoacacia L. with two different types of hyperaccumulator could effectively uptake and accumulate Cd and As to reduce the bioavailability of Cd and As in the contaminated soil, and effectively improve the soil environmental quality, which could be considered as a promising intercropping model for the simultaneous remediation of Cd and As contaminated soil in mining areas.

导出引用

李昊, 雷灿, 王德正, 佘迪凡, 曾鹏, 杜博研, 周航, 辜娇峰, 廖柏寒. 刺槐间作超富集植物修复镉砷复合污染土壤潜力研究[J]. 中国环境科学. 2025, 45(5): 2620-2630

LI Hao, LEI Can, WANG De-zheng, SHE Di-fan, ZENG Peng, DU Bo-yan, ZHOU Hang, GU Jiao-feng, LIAO Bo-han. Phytoremediation potential of Cd and As co-contaminated soil by Robinia pseudoacacia L. intercropped with hyperaccumulators[J]. China Environmental Science. 2025, 45(5): 2620-2630

中图分类号： X53

参考文献

[1] 晏利晶,姜淼,赵庆良,等.基于Meta分析的中国工矿业场地土壤重金属污染评价[J]. 环境科学研究, 2023,36(1):9-18. Yan L J, Jiang M, Zhao Q L, et al. Evaluation of soil heavy metal pollution in China’s industrial and mining sites based on meta-analysis [J]. Research of Environmental Sciences, 2023,36(1):9-18.
[2] Parker G H, Gillie C E, Miller J V, et al. Human health risk assessment of arsenic, cadmium, lead, and mercury ingestion from baby foods [J]. Toxicology Reports, 2022,9:238-249.
[3] Shah V, Daverey A. Phytoremediation: A multidisciplinary approach to clean up heavy metal contaminated soil [J]. Environmental Technology & Innovation, 2020,18:100774.
[4] Yaashikaa P R, Kumar P S, Jeevanantham S, et al. A review on bioremediation approach for heavy metal detoxification and accumulation in plants [J]. Environmental Pollution, 2022,301:119035.
[5] Rascio N, Navari-Izzo F. Heavy metal hyperaccumulating plants: How and why do they do it? And what makes them so interesting? [J]. Plant Science, 2011,180(2):169-181.
[6] Zou W T, Cao Z, Wang Y J, et al. Intercropping of Pennisetum sinese with Lolium perenne improved phytoextraction of heavy metal from soil [J]. Restoration Ecology, 2023,31(3):e13702.
[7] Zeng W B, Wan X M, Lei M, et al. Intercropping of Pteris vittata and maize on multimetal contaminated soil can achieve remediation and safe agricultural production [J]. Science of the Total Environment, 2024,915:170074.
[8] Guo Z H, Zeng P, Xiao X Y, et al. Physiological, anatomical, and transcriptional responses of mulberry (Morus alba L.) to Cd stress in contaminated soil [J]. Environmental Pollution, 2021,284:117387.
[9] Zeng P, Guo Z H, Xiao X Y, et al. Tolerance capacities of Broussonetia papyrifera to heavy metal(loid)s and its phytoremediation potential of the contaminated soil [J]. International Journal of Phytoremediation, 2022,24(6):580-589.
[10] Qu H J, Ma C X, Xiao J, et al. Co-planting of Quercus nuttallii, Quercus pagoda with Solanum nigrum enhanced their phytoremediation potential to multi-metal contaminated soil [J]. International Journal of Phytoremediation, 2021,23(10):1104-1112.
[11] 曾鹏,郭朝晖,肖细元,等.芦竹和木本植物间种修复重金属污染土壤[J]. 环境科学, 2018,39(11):5207-5216. Zeng P, Guo Z H, Xiao X Y, et al. Intercropping Arundo donax with woody plants to remediate heavy metal contaminated soil [J]. Environmental Science, 2018,39(11):5207-5216.
[12] 曾鹏,郭朝晖,肖细元,等.构树修复对重金属污染土壤环境质量的影响[J]. 中国环境科学, 2018,38(7):2639-2645. Zeng P, Guo Z H, Xiao X Y, et al. Effect of phytoremediation with Broussonetia papyrifera on the biological quality in soil contaminated with heavy metals [J]. China Environmental Science, 2018,38(7): 2639-2645.
[13] Zeng P, Guo Z H, Xiao X Y, et al. Complementarity of co-planting a hyperaccumulator with three metal(loid)-tolerant species for metal(loid)-contaminated soil remediation [J]. Ecotoxicology and Environmental Safety, 2019,169:306-315.
[14] Zhang C, Zha Y, Guang P, et al. Synergistic effects of tree-herb intercropping on the phytoremediation efficiency of cadmium and lead contaminated soil [J]. The Science of the total environment, 2024, 954:176709.
[15] Zeng P, Guo Z H, Xiao X Y, et al. Facilitation of Morus alba L. intercropped with Sedum alfredii H. and Arundo donax L. on soil contaminated with potentially toxic metals [J]. Chemosphere, 2022, 290:133107.
[16] 刘金秀,张松彦,周建,等.镉胁迫对刺槐幼苗生长与光合生理特性的影响[J]. 林业科学研究, 2023,36(3):168-178. Liu J X, Zhang S Y, Zhou J, et al. Effects of cadmium stress on growth and photosynthetic physiological characteristics of Robinia pseudoacacia seedlings [J]. Forestry Research, 2023,36(3):168-178.
[17] 魏树和,周启星,王新.超积累植物龙葵及其对镉的富集特征[J]. 环境科学, 2005,26(3):167-171. Wei S H, Zhou Q X, Wang X. Cadmium-hyperaccumulator Solanum nigrum L. and its accumulating characteristics [J]. Environmental Science, 2005,26(3):167-171.
[18] Zhao F J, Dunham S J, Mcgrath S P. Arsenic hyperaccumulation by different fern species [J]. New Phytologist, 2002,156(1):27-31.
[19] 鲁如坤.土壤农业化学分析方法[M]. 北京:中国农业科技出版社, 2000. Lu R K. Soil and agricultural chemistry analysis [M]. Beijing:China Agriculture Press, 2000.
[20] T/HNNMIA XX-2020土壤重金属元素可提取态氯化钙法[S]. T/HNNMIA XX-2020 Soil-Extractable state of heavy metal elements-Calcium chloride method [S].
[21] HJ 804-2016土壤8种有效态元素的测定二乙烯三胺五乙酸浸提-电感耦合等离子体发射光谱法[S]. HJ 804-2016 Soil-Determination of bioavailable form of eight elements-Extraction with buffered DTPA solution/Inductively coupled plasma optical emission spectrometry [S].
[22] Ma L Y, Huang L K, Liu Q Z, et al. Positive effects of applying endophytic bacteria in eggplant-Sedum intercropping system on Cd phytoremediation and vegetable production in cadmium polluted greenhouse [J]. Journal of Environmental Sciences, 2022,115:383-391.
[23] Xie Y D, Wang L M, Yang L X, et al. Intercropping with Eclipta prostrata and Crassocephalum crepidioides decrease cadmium uptake of tomato seedlings [J]. International Journal of Environmental Analytical Chemistry, 2021,101(9):1231-1239.
[24] 秦丽,何永美,王吉秀,等.续断菊与玉米间作的铅累积及根系低分子量有机酸分泌特征研究[J]. 中国生态农业学报, 2020,28(6): 867-875. Qin L, He Y M, Wang J X, et al. Lead accumulation and lowmolecular-weight organic acids secreted by roots in Sonchus asper L.-Zea mays L. intercropping system [J]. Chinese Journal of EcoAgriculture, 2020,28(6):867-875.
[25] 童文彬,曹雪蕊,江建锋,等.超积累东南景天与油料作物轮间作修复农田镉铅污染的技术模式研究[J]. 浙江大学学报(农业与生命科学版), 2021,47(2):212-222. Tong W B, Cao X R, Jiang J F, et al. Study on the remediation patterns of rotation or intercropping between hyperaccumulator Sedum alfredii and oil crops in cadmium and lead co-contaminated agricultural soils [J]. Journal of Zhejiang University: Agriculture and Life Sciences, 2021,47(2):212-222.
[26] 贺庭,刘婕,朱宇恩,等.重金属污染土壤木本-草本联合修复研究进展[J]. 中国农学通报, 2012,28(11):237-242. He T, Liu J, Zhu Y E, et al. Research progress on remediation of heavy metal pollution in soil by woody plants [J]. Chinese Agricultural Science Bulletin, 2012,28(11):237-242.
[27] Wang X H, Zhou C, Xiao X Y, et al. Phytoextraction potential of arsenic and cadmium and response of rhizosphere microbial community by intercropping with two types of hyperaccumulators [J]. Environmental Science and Pollution Research, 2022,29(60):91356-91367.
[28] Li Z R, Wang J X, An L Z, et al. Effect of root exudates of intercropping Vicia faba and Arabis alpina on accumulation and sub-cellular distribution of lead and cadmium [J]. International Journal of Phytoremediation, 2019,21(1):4-13.
[29] Wen W, Zhao H Y, Ma J L, et al. Effects of mutual intercropping on Pb and Zn accumulation of accumulator plants Rumex nepalensis, Lolium perenne and Trifolium repens [J]. Chemistry and Ecology, 2018,34(3):259-271.
[30] 张佳,孙传宇,何晓满,等.黑麦草间作对镉在小麦中积累和转运的影响[J]. 环境污染与防治, 2019,41(5):505-509,514. Zhang J Sun C Y, He X M, et al. Intercropping of ryegrass affects the Cd accumulation and transport in wheat [J]. Environmental Pollution & Control, 2019,41(5):505-509,514.
[31] Haichar F E Z, Marol C, Berge O, et al. Plant host habitat and root exudates shape soil bacterial community structure [J]. The ISME journal, 2008,2(12):1221-1230.
[32] Yao W B, Huang L, Yang Z H, et al. Effects of organic acids on heavy metal release or immobilization in contaminated soil [J]. Transactions of Nonferrous Metals Society of China, 2022,32(4):1277-1289.
[33] 尹晓童,杨浩,于瑞鹏,等.根系分泌物在作物多样性体系中对种间地下部互作的介导作用[J]. 中国生态农业学报, 2022,30(8):1215-1227. Yin X T, Yang H, Yu R P, et al. Interspecific below-ground interactions driven by root exudates in agroecosystems with diverse crops [J]. Chinese Journal of Eco-Agriculture, 2022,30(8):1215-1227.
[34] 高松,童新元,王芸,等.淋洗与电化学还原联用处理镉-铅污染土壤[J]. 中国环境科学, 2022,42(4):1788-1794. Gao S, Tong X Y, Wang Y, et al. Remediation of Cd-Pb contaminated soil by coupled soil washing and electrochemical reduction [J]. China Environmental Science, 2022,42(4):1788-1794.
[35] Zeng P, Guo Z H, Xiao X Y, et al. Phytoextraction potential of Pteris vittata L. co-planted with woody species for As, Cd, Pb and Zn in contaminated soil [J]. Science of the Total Environment, 2019,650:594-603.
[36] Lu Q Y, Li J H, Chen F B, et al. Effects of mutual intercropping on the cadmium accumulation in accumulator plants Stellaria media, Malachium aquaticum, and Galium aparine [J]. Environmental Monitoring and Assessment, 2017,189(12):622.
[37] 郭思宇,王海娟,王宏镔.重金属污染土壤间作修复的研究进展[J]. 中国生态农业学报, 2021,29(5):890-902. Guo S Y, Wang H J, Wang H B. Advances in the intercropping remediation of heavy metal polluted soil [J]. Chinese Journal of Eco-Agriculture, 2021,29(5):890-902.
[38] Li H G, Shen J B, Zhang F S, et al. Phosphorus uptake and rhizosphere properties of intercropped and monocropped maize, faba bean, and white lupin in acidic soil [J]. Biology and Fertility of Soils, 2010,46(2):79-91.
[39] Schwerdtner U, Spohn M. Plant Species Interactions in the Rhizosphere Increase Maize N and P Acquisition and Maize Yields in Intercropping [J]. Journal of Soil Science and Plant Nutrition, 2022, 22(3):3868-3884.
[40] 徐双圆,朱家辉,王栋茹,等.植物套作系统修复镉污染农田土壤的效应——以苏南地区为例[J]. 中国环境科学, 2024,44(06):3289-3300. Xu S Y, Zhu J H, Wang D R, et al. Effect of plant intercropping system on remediation of cadmium contaminated farmland soil—A case study on the southern Jiangsu province area [J]. China Environmental Science, 2024,44(6):3289-3300.
[41] Xia H, Liang D, Chen F B, et al. Effects of mutual intercropping on cadmium accumulation by the accumulator plants Conyza canadensis, Cardamine hirsuta, and Cerstium glomeratum [J]. International Journal of Phytoremediation, 2018,20(9):855-861.
[42] Chi G Y, Fang Y T, Zhu B, et al. Intercropping with Brassica juncea L. enhances maize yield and promotes phytoremediation of cadmiumcontaminated soil by changing rhizosphere properties [J]. Journal of Hazardous Materials, 2024,461:132727.
[43] 谭建波,湛方栋,刘宁宁,等.续断菊与蚕豆间作下土壤部分化学特征与Cd形态分布状况研究[J]. 农业环境科学学报, 2016,35(1):53-60. Tan J B, Zhan F D, Liu N N, et al. Soil chemical properties and Cd form distribution in Vicia faba and Sonchus asper intercropping system [J]. Journal of Agro-Environment Science, 2016,35(1):53-60.
[44] Fan F, Zhang F, Song Y, et al. Nitrogen Fixation of Faba Bean (Vicia faba L.) Interacting with a Non-legume in Two Contrasting Intercropping Systems [J]. Plant and Soil, 2006,283(1/2):275-286.
[45] Ng C W W, San So P, Wong J T F, et al. Intercropping of Pinellia ternata (herbal plant) with Sedum alfredii (Cd-hyperaccumulator) to reduce soil cadmium (Cd) absorption and improve yield [J]. Environmental Pollution, 2023,318:120930.
[46] 孟楠,王萌,陈莉,等.不同草本植物间作对Cd污染土壤的修复效果[J]. 中国环境科学, 2018,38(7):2618-2624. Meng N, Wang M,Chen L, et al. Remediation efficiency of Cd polluted soil by intercropping with herbaceous plants [J]. China Environmental Science, 2018,38(7):2618-2624.
[47] 丁馨茹,徐曼,严宁珍,等.4种改良剂对紫色土Cd生物有效性及土壤酶活性的影响[J]. 环境科学, 2024,45(6):3523-3532. Ding X R, Xu M, Yan N Z, et al. Effects of four amendments on cadmium bioavailability and enzyme activity in purple soil [J]. Environmental Science, 2024,45(6):3523-3532.
[48] Zeng P, Guo Z H, Xiao X Y, et al. Dynamic response of enzymatic activity and microbial community structure in metal(loid)-contaminated soil with tree-herb intercropping [J]. Geoderma, 2019,345:5-16.
[49] Vepsäläinen M, Kukkonen S, Vestberg M, et al. Application of soil enzyme activity test kit in a field experiment [J]. Soil Biology & Biochemistry, 2001,33(12/13):1665-1672.
[50] Shen C C, Wang J, Jing Z W, et al. Plant diversity enhances soil fungal network stability indirectly through the increase of soil carbon and fungal keystone taxa richness [J]. Science of the Total Environment, 2022,818:151737.
[51] 闫秀秀,徐应明,王林,等.叶用油菜和孔雀草间作对植物生长和镉累积的影响[J]. 环境科学, 2020,41(11):5151-5159. Yan X X, Xu Y M, Wang L, et al. Effects of Intercropping of Brassica chinenesis L. and Tagetes patula L. on the growth and cadmium accumulation of plants [J]. Environmental Science, 2020,41(11): 5151-5159.
[52] 吴乐诗,余柏仲,李冬琴,等.菜心与东南景天间作修复镉污染农田的生态效应[J]. 农业环境科学学报, 2024,43(7):1483-1491. Wu L S, Yu B Z, Li D Q, et al. Ecological effects of intercropping Brassica parachinensis and Sedum alfredii on the remediation of cadmium contaminated farmland [J]. Journal of Agro-Environment Science, 2024,43(7):1483-1491.
[53] 徐华勤,肖润林,向佐湘,等.稻草覆盖、间作三叶草茶园土壤酶活性与养分的关系[J]. 生态学杂志, 2009,28(8):1537-1543. Xu H Q, Xiao R L, Xiang Z X, et al. Soil enzyme activities and their relations with soil fertility in a tea plantation under straw mulching and white clover intercropping [J]. Chinese Journal of Ecology, 2009,28(8):1537-1543.