酸性矿山废水灌溉对稻田土壤铁碳的影响及微生物群落结构响应

刘健; 牛少勋; 黄恩惠; 刘振聪; 李晓飞; 吴小莲; 柯常栋; 包艳萍

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (5) : 2654-2663.

土壤污染与控制

酸性矿山废水灌溉对稻田土壤铁碳的影响及微生物群落结构响应

刘健¹, 牛少勋¹, 黄恩惠¹, 刘振聪¹, 李晓飞¹, 吴小莲¹, 柯常栋², 包艳萍¹

作者信息 +

Effects of acid mine drainage irrigation on iron and carbon in paddy soils and the response of microbial community structure

LIU Jian¹, NIU Shao-xun¹, HUANG En-hui¹, LIU Zheng-cong¹, LI Xiao-fei¹, WU Xiao-lian¹, KE Chang-dong², BAO Yan-ping¹

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文章历史 +

摘要

以广东大宝山矿区酸性矿山废水(AMD)长期灌溉的稻田土壤为研究对象进行铁形态及铁碳结合特征分析,结合高通量测序技术,揭示AMD灌溉铁输入对稻田土壤有机碳固定的影响及微生物群落结构响应.结果表明:①AMD灌溉导致稻田土壤酸化,铁硫元素及重金属在土壤中积累,土壤总有机碳(TOC)与总铁(TFe)、铁结合态有机碳(OC_Fe)呈极显著正相关.②AMD灌溉导致土壤微生物丰度和多样性降低,AMD污染土壤Geobacter相对丰度下降,形成了以耐酸的铁硫代谢菌Thiobacillus和Sulfurifustis等为主要优势属的微生物群落结构.③RDA分析表明影响稻田土壤微生物群落结构组成的主要环境因子为络合态铁(Fe_p)、TOC和TFe.研究显示,AMD灌溉引入的铁有利于稻田土壤有机碳的保存,同时AMD灌溉导致稻田土壤形成了与污染程度、铁碳形态及含量紧密相关联的微生物群落结构.

Abstract

This study focused on the long-term AMD-contaminated paddy soils in the Dabaoshan mining area of Guangdong Province. The distribution characteristics of soil iron phases and iron-bound organic carbon were analysed, combining with high-throughput sequencing to examine the effects of AMD irrigation on soil organic carbon sequestration and the response of soil microbial community structure. The results indicated that: ① AMD irrigation led to soil acidification, with accumulation of iron, sulfur and heavy metals in paddy soils. The contents of TOC in paddy soils showed significant positive correlation with TFe, and OC_Fe. ② AMD irrigation resulted in decreases of soil microbial abundance and diversity. AMD irrigation led to a decrease in the relative abundance of Geobacter in paddy soils, whereas acid tolerant iron and/or sulfur metabolizing bacteria such as Thiobacillus and Thioifustis became the dominant bacteria in paddy soils with the most heavily AMD pollution. ③ RDA analysis identified Fe_p, TOC, and TFe were the most crucial factors influencing microbial community structure. In conclusion, AMD irrigation brought dissolved iron into paddy soils which was beneficial to soil organic carbon preservation. In addition, AMD irrigation resulted in the formation of microbial community structure that closely related to AMD pollution gradient, the form and content of iron and carbon.

导出引用

刘健, 牛少勋, 黄恩惠, 刘振聪, 李晓飞, 吴小莲, 柯常栋, 包艳萍. 酸性矿山废水灌溉对稻田土壤铁碳的影响及微生物群落结构响应[J]. 中国环境科学. 2025, 45(5): 2654-2663

LIU Jian, NIU Shao-xun, HUANG En-hui, LIU Zheng-cong, LI Xiao-fei, WU Xiao-lian, KE Chang-dong, BAO Yan-ping. Effects of acid mine drainage irrigation on iron and carbon in paddy soils and the response of microbial community structure[J]. China Environmental Science. 2025, 45(5): 2654-2663

中图分类号： X53

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