现有海洋污染的生物毒性检测方法均存在毒性响应时间长,难以实现海洋污染物快速响应难题.以具有能动性的海洋微藻亚心形扁藻为受试生物,研究了其运动性对海洋典型重金属污染物汞(Hg)和铬(Cr)的毒性响应性能,以期为建立海洋生物毒性快速检测方法提供依据.结果表明:2h内,亚心形扁藻的运动参数,包括运动方式、运动能力和游泳速度对0.075~2.5mg/L Hg和0.1~3mg/L Cr均产生了明显的响应,运动性参数与两种重金属浓度间都呈现良好的剂量-效应关系;基于不同运动性参数获得Hg和Cr的2h-EC50值分别为0.63~2.38mg/L和0.60~2.49mg/L;总体上,不同运动参数中曲线运动速度(VSL)对重金属Hg和Cr毒性具有最灵敏的响应,以VSL为响应指标获得的Hg和Cr的2h胁迫下的半抑制浓度值(2h-EC50值)分别为0.63和0.60mg/L.上述结果与传统毒性试验(包括微藻72h生长抑制试验、24~48h光合抑制试验和96h鱼类死亡试验)结果均具有可比性,且毒性响应时间大大缩短,表明微藻运动性作为一种新型生物测试指标可以对海洋重金属类污染物毒性进行快速、有效评价.
Abstract
The rapid detection of biological toxicity of Marine pollution is of great significance to the emergency monitoring of sudden Marine water pollution accidents, the rapid investigation and evaluation of Marine environment ecological risks, and the protection of Marine environment. However, existing biological toxicity detection methods have a long toxic response time, and it is difficult to realize the rapid response of Marine pollutants. In view of this, this study has taken the active marine microalgae, Platymonas subcordiformis, as the test organism to study its mobility response to typical heavy metal pollutants Mercury (Hg) and Chromium (Cr) in seawater, in order to provide a basis for establishing a rapid detection method for marine biological toxicity. The results ha showed that within 2h, the motion parameters of the Platymonas subcordiformis, including movement mode, movement ability, and swimming velocity, exhibited significant response characteristics to 0.075 ~ 2.5mg/L Hg and 0.1 ~ 3mg/L Cr, and there was a good dose-response relationship between the motion parameters and the concentrations of the two independent heavy metals; The 2h-EC50 values of Hg and Cr obtained based on different motion parameters were 0.63 ~ 2.38mg/L and 0.60 ~ 2.49mg/L, respectively; Overall, among different motion parameters, the curve velocity (VSL) has the most sensitive response to the toxicity of heavy metals Hg and Cr. The 2h-EC50 values of Hg and Cr obtained using VSL as the response index are 0.63 and 0.60mg/L respectively. The above results are comparable to traditional toxicity tests (including microalgae 72-hour growth inhibition test, 24 ~ 48 hours photosynthesis inhibition test, and 96-hour fish death test), and the toxicity response time is significantly reduced, indicating that microalgae motility as a new biological testing indicator can quickly and effectively evaluate the toxicity of marine heavy metal pollutants.
关键词
海洋微藻 /
海洋污染 /
生物毒性 /
运动性 /
重金属
Key words
biological toxicity /
heavy metals /
marine microalgae /
marine pollution /
motility
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 刘慧杰,刘文君,刘继平,等.南中国海表层海水重金属含量及其潜在生态风险分析[J].中国环境科学, 2017,37(10):3891-3898. Liu H J, Liu W J, Liu J P, et al. Heavy metals concentration and its potential ecological risk assessment of surface seawater in South China Sea[J]. China Environmental Science, 2017,37(10):3891-3898.
[2] 黄向青,张顺枝,霍振海.深圳大鹏湾、珠江口海水有害重金属分布特征[J].海洋湖沼通报, 2005,(4):38-44. Huang X Q, Zhang S Z, Huo Z H, et al. Heavy metal distribution in seawater in Dapeng Bay of Shenzhen and the Pearl River estuary[J]. Transactions of Oceanology and Limnology, 2005,(4):38-44.
[3] 齐凤霞,郑丙辉,万峻,等.渤海湾(天津段)柱样沉积物重金属污染研究[J].海洋技术, 2004,(3):85-91. Qi F X, Zheng B H, Wan J, et al. Pollution of Heavy metal in the sample of column of Bohai Bay in Tianjin[J]. Journal of Ocean Technology, 2004,(3):85-91.
[4] 黄厚见,平仙隐,李磊,等.春、夏季长江口海水、沉积物及生物体中重金属含量及其评价[J].生态环境学报, 2011,20(5):898-903. Huang H J, Ping X Y, Li L, et al. Concentrations and assessment of heavy metals in seawater, sediments and aquatic organisms at Yangtze River estuary in spring and summer[J]. Ecology and Environmental Sciences, 2011,20(5):898-903.
[5] 康凯莉,管博,李正炎.中国近海环境中汞的水质基准与生态风险[J].中国海洋大学学报(自然科学版), 2019,49(1):102-114. Kang K L, Guan B, Li Z Y, et al. Marine water quality criteria derivation and ecological risk assessment for mercury in coastal waters in China[J]. Periodical of Ocean University of China, 2019,49(1): 102-114.
[6] 季兴财.莆田市平海湾地下水质量现状及污染因子贡献度分析[J].黑龙江环境通报, 2019,43(4):23-26,59. Ji X C. Analysis on present situation and the contribution degree of polution factors of grundwater quality in Pinghai Bay, Putian[J]. Heilongjiang Environmental Journal, 2019,43(4):23-26,59.
[7] 陈方涛,李志勇,张扬,等.渤海海洋环境污染的现状与防治浅谈[J].资源节约与环保, 2023,(1):9-13,42. Chen F T, Li Z Y, Zhang Y, et al. Discussion on the current situation and prevention and control of Marine Environmental pollution in Bohai Sea[J]. Resources Economization& Environmental Protection, 2023,(1):9-13,42.
[8] 秦捷,赵文,张鹏.环境汞污染对藻类的毒性效应及其影响因素[J].生物学杂志, 2011,28(3):74-76,83. Qin J, Zhao W, Zhang P. The environment mercury pollution toxicity effect to the alga and their influencing factors[J]. Journal of Biology, 2011,28(3):74-76,83.
[9] 陈冉冉,朱倩,赵敏,等.两种价态铬(Ⅲ,Ⅵ)对真核酵母细胞谷胱甘肽水平影响[J].工业微生物, 2020,50(2):36-40. Chen R R, Zhu Q, Zhao M, et al. Effects of two kinds of chromium (Ⅲ,Ⅵ) on glutathione levels in eukaryotic yeast cells[J]. Industrial Microbiology, 2020,50(2):36-40.
[10] 刘原源,曾威阳,陈如,等.微藻趋向运动及其靶向运输应用研究进展[J].生物工程学报, 2022,38(2):578-591. Liu S Y, Zeng W Y, Chen R, et al. Tactic movement of microalgae and its application in targeted transport: a review[J]. Chinese Journal of Biotechnology, 2022,38(2):578-591.
[11] 朱政.自旋运动型微藻细胞的空间自组织行为探究[D].武汉:华东师范大学, 2019. Zhu Z. Spatial self-organized behaviors of self-spinning microalgae[D]. Wuhan: East China Normal University, 2019.
[12] Liang Y, Zhu X, Wu Q, et al. Ciliary length sensing regulates IFT entry via changes in FLA8/KIF3B phosphorylation to control ciliary assembly[J]. Current Biology, 2018,28(15):2429-2435.
[13] Feng C Y, Wei J F, Li Y J, et al. An on-chip pollutant toxicity determination based on marine microalgal swimming inhibition[J]. Analyst, 2016,141(5):1761-1771.
[14] Zhao X, Lin X, Qu K, et al. Toxicity of BDE-47, BDE-99and BDE-153on swimming behavior of the unicellular marine microalgae Platymonas subcordiformis and implications for seawater quality assessment[J]. Ecotoxicology and Environmetal Safety, 2019,174: 408-416.
[15] Park J, Lee H, Depuydt S, et al. Assessment of five live-cell characteristics in periphytic diatoms as a measure of copper stress[J]. Journal of Hazardous Materials, 2020,400:123113.
[16] Huang L, Zhang W X, Zhou W S, et al. Behaviour, a potential bioindicator for toxicity analysis of waterborne microplastics: A review[J]. TrAC Trends in Analytical Chemistry, 2023,162:117044.
[17] Wang T, Yin G F, Zhao N J. A multi-target tracking method for phytoplankton based on adaptive buffer intersection-over-union[C] //20245th International Seminar on Artificial Intelligence, Networking and Information Technology (AINIT). Nanjing: IEEE, 2024.
[18] Cutler G C, Amichot M., Benelli G., et al. Hormesis and insects: Effects and interactions in agroecosystems[J]. Science of the Total Environment, 2022,825:153899.
[19] Rix R R, Cutler G C. Review of molecular and biochemical responses during stress induced stimulation and hormesis in insects[J]. Science of the Total Environment, 2022,827:154085.
[20] Agathokleous E, Kitao M, Calabrese E J. Hormetic dose responses induced by lanthanum in plants[J]. Environmental Pollution, 2019, 244:332–341.
[21] Zhang Y, Gao Q, Liu S, et al. Hormetic dose-response of halogenated organic pollutants on Microcystis aeruginosa: Joint toxic action and mechanism[J]. Science of the Total Environment, 2022,829:154581.
[22] Tang W X, He M, Chen B B, et al. Investigation of toxic effect of mercury on Microcystis aeruginosa: Correlation between intracellular mercury content at single cells level and algae physiological responses[J]. The Science of the Total Environment, 2022,858(P2):159894- 159894.
[23] Du X Y, Zhou W S, Zhang W X, et al. Toxicities of three metal oxide nanoparticles to a marine microalga: Impacts on the motility and potential affecting mechanisms[J]. Environmental Pollution, 2021, 290:118027-118027.
[24] 夏亦雪,艾晓寒,朱飞霞,等.不同衣藻光合作用响应汞胁迫的比较研究[J].水生生物学报, 2024,48(6):1040-1050. Xia Y X, Ai X H, Zhu F X, et al. Comparative of photpsynthesis of different Chlamydomonas reinhardtii in response to Mercury stress[J]. Acta Hydrobiologica Sinica, 2024,48(6):1040-1050.
[25] 逯南南,孙韶华,宋武昌,等.基于斑马鱼行为变化的水体突发汞污染物预警技术研究[J].安全与环境, 202016,23(5):69-72,79. Lu N N, Sun S H, Song W C, et al. Technology of behavior-based biological warning of sudden mercury pollution in water[J]. Safety and Environmental Engineering, 202016,23(5):69-72,79.
[26] 李燕,朱琳,刘硕.铅、汞单一及联合胁迫对栅藻的生长、GSH含量及相关酶活性的影响[J].环境科学, 2009,30(1):248-253. Li Y, Zhu L, Liu S. Individual and joint stress of lead and mercury on growth, glutathione and glutathione-related enzymes of scenedesmus quadricauda[J]. Environmental Science, 2009,30(1):248-253.
[27] Raj M V, Thirunavukkarasu R A, Kailasam M, et al. Acute toxicity bioassays of cadmium and mercury on the juveniles of Asian SeabassLates calcarifer (Bloch)[J]. Indian Journal of Science and Technology, 2013,6(4):4329-4335.
基金
国家自然科学基金项目(62375270);合肥综合性科学中心环境研究院科研团队建设项目(HYKYTD2024004);皖江新兴产业技术发展中心科研项目(WJ23CYHXM03);国家工程研究中心开放基金(2024KFJJ0108);珠海市产学研合作项目(2320004002782);中国仪器仪表学会科学仪器托举计划项目(CISTJ2024)
PDF(1522 KB)
京公网安备 11010802030352号 
