在废水的脱氮除磷过程中,通过合作避免优势功能微生物的共生竞争是深度脱氮除磷的有效策略.本研究采用单级序批式反应器以厌氧/缺氧/好氧/缺氧(A/A1/O/A2)的模式运行100d,调节聚磷菌(PAOs)、反硝化聚磷菌(DPAOs)和反硝化聚糖菌(DGAOs)的动态平衡,以期实现高效脱氮除磷.结果表明,采用A/A1/O/A2模式的新型反硝化除磷(DPR)系统为不同功能微生物创造了协同环境,通过增设回流装置和缩短有氧段时间的优化,氮去除效率(NRE)和磷去除效率(PRE)最终分别达到了(95.13%±0.35%)和(94.70%±0.96%),实现了良好的脱氮除磷效果.机理分析表明,新型DPR系统中,好氧段前的A1段可能为DPAOs创造了良好的缺氧环境,DPAOs在此进行反硝化除磷,而在A2段,DGAOs可能在聚羟基烷链酸(PHAs)和糖原(Gly)驱动下实现内源反硝化脱氮.胞外聚合物(EPS)结果分析表明,相较于以A/O/A模式运行的R1,以A/A1/O/A2模式运行的R2中蛋白质(PN)和多糖(PS)含量分别提高了35.38和12.39mg/gVSS,污泥聚集性得到增强.此外,对微生物群落结构的分析表明,Dechloromonas和Ca.Competibacter的丰度分别从R1的2.24%、1.53%上升到R2的7.61%、7.94%和R3的4.62%、5.28%,A/A1/O/A2模式显著提高了主要DPAOs菌属和主要DGAOs菌属的丰度.
Abstract
Cooperative strategies that mitigate competition among dominant functional microorganisms are crucial for efficient nitrogen and phosphorus removal in wastewater treatment. This study investigated a novel single-stage sequencing batch reactor operating under an anaerobic/anaerobic/oxic/anaerobic (A/A1/O/A2) mode for 100days to regulate the dynamic balance of phosphorus-accumulating organisms (PAOs), denitrifying PAOs (DPAOs), and denitrifying glycogen-accumulating organisms (DGAOs). The optimized system, featuring a recycle loop and reduced aerobic phase duration, achieved nitrogen and phosphorus removal efficiencies of (95.13%±0.35%) and (94.70%±0.96%), respectively. Mechanistic analysis suggested that the A1 phase created an anoxic environment conducive to DPAO-mediated denitrifying phosphorus removal, while the A2 phase supported DGAO-driven denitrifying nitrogen removal using polyhydroxyalkanoates (PHAs) and glycogen (Gly). Extracellular polymeric substance (EPS) analysis revealed increases of 35.38mg/gVSS in protein (PN) and 12.39mg/gVSS in polysaccharide (PS) content, enhancing sludge aggregation. Microbial community analysis demonstrated significant enrichment of Dechloromonas and Ca. Competibacter, with their abundances increasing from 2.24% and 1.53% in R1to 7.61% and 7.94% in R2, respectively. The A/A1/O/A2 mode effectively created a synergistic environment for key DPAOs and DGAOs, achieving superior nitrogen and phosphorus removal performance compared to conventional modes.
关键词
EPS /
反硝化除磷 /
内源反硝化 /
脱氮除磷
Key words
denitrifying phosphorus removal /
endogenous denitrification /
EPS /
nitrogen and phosphorus removal
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基金
北京高校卓越青年科学家计划项目(BJJWZYJH01201910005 019)
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