急性Cu(Ⅱ)与Cr(Ⅵ)对Anammox脱氮及微生物群落的影响

傅慧敏; 白继春; 刘文科; 陈猷鹏; 程顺利; 邢崇阳

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (6) : 3020-3027.

水污染与控制

急性Cu(Ⅱ)与Cr(Ⅵ)对Anammox脱氮及微生物群落的影响

傅慧敏¹, 白继春¹, 刘文科¹, 陈猷鹏², 程顺利³, 邢崇阳^1,4

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Acute effects of Cu(II) and Cr(VI) on anammox nitrogen removal performance and microbial community structure

FU Hui-min¹, BAI Ji-chun¹, LIU Wen-ke¹, CHEN You-peng², CHENG Shun-li³, XING Chong-yang^1,4

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摘要

本文研究了高浓度重金属离子Cu(Ⅱ)和Cr(Ⅵ)对厌氧氨氧化(anammox)污泥脱氮效率、微生物群落和菌群代谢的短期急性影响.结果表明,12mg/L Cu(Ⅱ)短期会对anammox颗粒污泥的脱氮性能产生抑制作用,暂时抑制脱氮能力.8mg/L Cr(Ⅵ)会对anammox颗粒脱氮性能产生显著的抑制,反应几乎停滞.高浓度Cu(Ⅱ)和Cr(Ⅵ)的添加导致反应器中anammox菌Candidatus_Kuenenia分别下降4.86%和2.88%,因此得出高浓度Cu(Ⅱ)和Cr(Ⅵ)可能通过抑制anammox菌的活性进而抑制anammox颗粒的脱氮性能.菌群功能预测分析表明anammox群落可能通过提高细胞运动性、新陈代谢、细胞运动过程和信号以及异种生物降解和代谢等方面代谢活性来抵抗Cu(Ⅱ)和Cr(Ⅵ)的压力.

Abstract

This study evaluated the short-term, acute impacts of elevated Cu(II) and Cr(VI) concentrations on the nitrogen-removal efficiency, microbial community composition, and predicted metabolic responses of anammox granular sludge. Results showed that 12mg/L Cu(II) induced a temporary inhibition of anammox activity. In contrast, 8mg/L Cr(VI) caused a near-complete cessation of nitrogen removal. High concentrations of Cu(II) and Cr(VI) decreased the relative abundance of Candidatus Kuenenia by 4.86% and 2.88%, respectively, indicating that heavy metals likely impair anammox performance by directly inhibiting key anammox bacteria. Functional-prediction analysis (PICRUSt2) suggested that, under Cu(II) and Cr(VI) stress, the anammox community upregulated pathways associated with cell motility, energy metabolism, chemotaxis, signal transduction, and xenobiotic biodegradation— presumably as adaptive responses to mitigate toxicity.

导出引用

傅慧敏, 白继春, 刘文科, 陈猷鹏, 程顺利, 邢崇阳. 急性Cu(Ⅱ)与Cr(Ⅵ)对Anammox脱氮及微生物群落的影响[J]. 中国环境科学. 2025, 45(6): 3020-3027

FU Hui-min, BAI Ji-chun, LIU Wen-ke, CHEN You-peng, CHENG Shun-li, XING Chong-yang. Acute effects of Cu(II) and Cr(VI) on anammox nitrogen removal performance and microbial community structure[J]. China Environmental Science. 2025, 45(6): 3020-3027

中图分类号： X703

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