喹啉/微塑料共存对Anammox生物膜反应器的影响

谢宇涵; 周鑫; 牛冰心

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (6) : 3037-3045.

水污染与控制

喹啉/微塑料共存对Anammox生物膜反应器的影响

谢宇涵, 周鑫, 牛冰心

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Effects of co-existence of quinoline/microplastics on anammox biofilm reactor

XIE Yu-han, ZHOU Xin, NIU Bing-xin

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摘要

本文采用厌氧序批式生物膜反应器探究了喹啉(50~200mg/L)和微塑料(PET-MPs)(20~100mg/L)共存条件下对厌氧氨氧化(Anammox)的复合影响机制.结果表明,随着喹啉和PET-MPs浓度增高,Anammox性能先下降,再逐步回升,且可逆性抑制恢复时间与复合污染物浓度呈正相关.厌氧氨氧化活性(SAA)从C1阶段的22.8mg N/(g VSS·h)下降至C3阶段的16.2mg N/(g VSS·h),而相应活性氧(ROS)产生量提高55.7%,表明复合污染下对Anammox抑制程度增强.胞外聚合物(EPS)分析发现喹啉和PET-MPs浓度增加会导致生物膜EPS含量从75.3mg/g VSS快速下降至39.2mg/g VSS,蛋白质(PN)分泌量减少显著进而导致PN/PS显著下降表明Anammox生物膜结构稳定性下降.高通量测序发现喹啉/PET-MPs浓度升高,微生物群落多样性和丰富度指数均下降,Candidatus_Brocadia相对丰度从1.73%下降至1.24%,Denitratisoma相对丰度变化不大,而厌氧杂环降解菌的相对丰度则显著增加.

Abstract

An anaerobic sequencing batch biofilm reactor was used to explore the combined effect mechanism of anammox under the co-existence conditions of quinoline (50~200mg/L) and microplastics (PET-MPs) (20~100mg/L). With the increase of the concentrations of quinoline and PET-MPs, the performance of Anammox first decreases and then gradually recovers, and recovery time of reversible inhibition was positively correlated with the concentration of combined pollutants. The specific anammox activity (SAA) decreased from 22.8mg N/(g VSS·h) in stage C1 to 16.2mg N/(g VSS·h) in stage C3, while the corresponding reactive oxygen species (ROS) production increased by 55.7%, indicating that the inhibition of Anammox was enhanced under combined pollution. Extracellular polymer (EPS) analysis revealed that an increase in the concentrations of quinoline and PET-MPs would lead to a rapid decrease in the EPS content of the biofilm from 75.3mg/g VSS to 39.2mg/g VSS. The significant reduction in protein (PN) secretion, which in turn led to a significant decrease in PN/PS, indicates a decline in the structural stability of the Anammox biofilm. High-throughput sequencing revealed that the concentration of quinoline /PET-MPs increased, while the microbial community diversity and richness indices decreased. The relative abundance of Candidatus_Brocadia decreased from 1.73% to 1.24%, while the relative abundance of Denitratisoma changed little. However, the relative abundance of anaerobic heterocyclic degrading bacteria increased significantly.

导出引用

谢宇涵, 周鑫, 牛冰心. 喹啉/微塑料共存对Anammox生物膜反应器的影响[J]. 中国环境科学. 2025, 45(6): 3037-3045

XIE Yu-han, ZHOU Xin, NIU Bing-xin. Effects of co-existence of quinoline/microplastics on anammox biofilm reactor[J]. China Environmental Science. 2025, 45(6): 3037-3045

中图分类号： X703

参考文献

[1] Luo Y H, Yue X P, Wei P, et al. A state-of-the-art review of quinoline degradation and technical bottlenecks [J]. Science of the Total Environment, 2020,747:141136.
[2] An X J, Li N J, Zhang L Z, et al. New insights into the typical nitrogen-containing heterocyclic compound-quinoline degradation and detoxification by microbial consortium:Integrated pathways, meta-transcriptomic analysis and toxicological evaluation [J]. Journal of Hazardous Materials, 2024,465:133158.
[3] Wu P, Chen J J, Garlapati V K, et al. Novel insights into Anammox- based processes:A critical review [J]. Chemical Engineering Journal Chemical Engineering Journal, 2022,444:136534.
[4] Akyildiz S H, Bellopede R, Sezgin H, et al. Detection and analysis of microfibers and microplastics in wastewater from a textile company [J]. Microplastics, 2022,1(4):572-586.
[5] Liu J, Yu Y, Chang Y, et al. Enhancing quinoline and phenol removal by adding Comamonas testosteroni bdq06 in treatment of an accidental dye wastewater [J]. International Biodeterioration & Biodegradation, 2016,115:74-82.
[6] Ramsperger A F, Stellwag A C, Caspari A, et al. Structural diversity in early-stage biofilm formation on microplastics depends on environmental medium and polymer properties [J]. Water, 2020,12(11), 3216.
[7] Vivekanand A C, Mohapatra S, Tyagi V K. Microplastics in aquatic environment: Challenges and perspectives [J]. Chemosphere, 2021, 282:131151.
[8] 洪先韬,周鑫,聚对苯二甲酸乙二醇酯微塑料对Anammox颗粒污泥的尺寸影响效应[J]. 中国环境科学, 2023,43(12):6406-6412.Hong X T, Zhou X, size effect of polyethylene terephthalate microplastics on Anammox granular sludge [J]. China Environmental Science, 2023,43(12):6406-6412.
[9] Chen Q Q, Xu L Z J, Zhang Z Z, et al. Insight into the short-and long-term effects of quinoline on anammox granules:inhibition and acclimatization [J]. Science of the Total Environment, 2019,651: 1294-1301.
[10] Hong X T, Niu B X, Sun H W, et al. insight into response characteristics and inhibition mechanisms of anammox granular sludge to polyethylene terephthalate microplastics exposure [J]. Bioresource Technology, 2023,385:129355.
[11] Lee I, Khujaniyoz S, Oh H, et al. The removal of microplastics from reverse osmosis wastewater by coagulation [J]. Journal of Environmental Chemical Engineering, 2024,12(4):113198.
[12] 李亚新,赵晨红.紫外分光光度法同时定量测定多组分混合物——喹啉、吡啶、吲哚、苯酚[J]. 环境工程, 1999,17(2):58-60.Li Y X, Zhao C H, UV spectrophotometry simultaneously quantifies multi-component mixtures – quinoline, pyridine, indole, phenol [J]. Environmental Engineering, 1999,17(2):58-60.
[13] Chen H, Zhang Z C, Jin R C, et al. Deciphering the short-term deactivation mechanism of the anammox performance under calcium stress [J]. Separation and Purification Technology, 2022,292:120988.
[14] Zhang P, Fang F, Chen Y P, et al. Composition of EPS fractions from suspended sludge and biofilm and their roles in microbial cell aggregation [J]. Chemosphere, 2014,117:59-65.
[15] Tan C, Chen S Y, Zhang H Y, et al The roles of Rhodococcus ruber in denitrification with quinoline as the electron donor [J]. Science of the Total Environment, 2023,902:166128.
[16] Ramos C, Fernandez I, Suarez-Ojeda M E, et al. Inhibition of the anammox activity by aromatic compounds [J]. Chemical Engineering Journal, 2015,279:681-688.
[17] Niu B X, Xie Y H, Sun H W, et al. Removal behavior, inhibition kinetics, microbial community and metabolic pathways in simultaneous anammox and denitrification under indole exposure [J]. Journal of Environmental Chemical Engineering, 2024,12:112296.
[18] Kinigopoulou V, Pashalidis I, Kalderis D, et al. Microplastics as carriers of inorganic and organic contaminants in the environment:A review of recent progress [J]. Journal of Molecular Liquids, 2022, 350:118580.
[19] Wang X, Wu Q, Wang Z Z, et al. Biotransformation-mediated detoxification of roxarsone in the anammox process:Gene regulation mechanism[J]. Chemical Engineering Journal, 2023,467:143449.
[20] Su Y, Yang H, Wang X T, et al. Successful enhancement and related characteristics of anammox bacterial activity by hydraulic supply strategy under limited inoculum [J]. Journal of Water Process Engineering, 2021,41:102005.
[21] 胡璇,陈滢.聚酯纤维PET-MPs胁迫下活性污泥系统性能及微生物群落的变化情况[J]. 化工进展, 2023,42(2):1051-1060.Hu X, Chen Y. Changes in the performance and microbial community of activated sludge system under polyester fiber microplastic stress [J]. Chemical Progress, 2023,42(2):1051-1060.
[22] Zhang J W, Peng Y Z, LI X C, et al. Feasibility of partial- denitrification/ anammox for pharmaceutical wastewater treatment in a hybrid biofilm reactor [J]. Water Research, 2022,208:117856.
[23] Zhao J, Zhang B W, Zuo J N, Response of anammox granules to ZnO nanoparticles at ambient temperature [J]. Environmental Technology & Innovation, 2019,13:146-152.
[24] Liu L J, Ji M, Wang F, et al. Response of nitrogen removal performance, functional genes abundances and N-acyl-homoserine lactones release to carminic acid of anammox biomass [J]. Bioresource Technology, 2020,299:122567.
[25] Huang Y, Peng Y Z, Huang D H, et al. Enhanced nitrogen removal from domestic wastewater by partial-denitrification/anammox in an anoxic/oxic biofilm reactor [J]. Processes, 2022,10(1):109.
[26] Hou Z L, Zhou X, Dong W Y, et al. Insight into correlation of advanced nitrogen removal with extracellular polymeric substances characterization in a step-feed three-stage integrated anoxic/oxic biofilter system [J]. Science of The Total Environment, 2022,806: 151418.
[27] Cheng Y F, Zhang Z Z, Ma W J, et al. Response of the mainstream anammox process to the biodegradable carbon sources in the granule-based systems: The difference in self-stratification of the microbial community [J]. Science of The Total Environment, 2022,851: 158191.
[28] Zhao D T, Xiong H, Shan X C, et al. Response of nitrogen removal and microbial community to high concentration of humic acid in the anammox biofilm system [J]. Journal of Water Process Engineering, 2023,53:103706.
[29] 张之钰,武海涛,刘吉平,等.PET-MPs对稻田土壤温室气体排放和微生物群落的影响[J]. 生态学报, 2024,44(10):4308-4318.Zhang Z Z, Wu H T, Liu J P, et al. Effects of microplastics on soil greenhouse gas emissions and microbial communities in paddy fields [J]. Acta Ecologica Sinica, 2024,44(10):4308-4318.
[30] Xiao H H, Wang K N, Wang Y L, et al. Inhibition of denitrification and enhancement of microbial interactions in the AGS system by high concentrations of quinoline [J]. Journal of Environmental Management, 2024,370:122837.
[31] Wang D P, Li T, Huang K L,et al, Roles and correlations of functional bacteria and genes in the start-up of simultaneous anammox and denitrification system for enhanced nitrogen removal [J]. Science of the Total Environment, 2019,655:1355-1363.
[32] 闫冰,聂含冰,付嘉琦,等.长期运行厌氧氨氧化系统沿程不同形态污泥微生物群落结构及功能解析[J]. 中国环境科学, 2025,45(2): 1052-1062.Yan B, Nie H B, Fu J Q, et al. Deciphering the microbial community structure and function of sludge with different morphologies along the ANAMMOX reactor under long-term operation [J]. China Environmental Science, 2025,45(2):1052-1062.
[33] Wang Y, Tian H, Huang F, et al Time-resolved analysis of a denitrifying bacterial community revealed a core microbiome responsible for the anaerobic degradation of quinoline [J]. Scientific reports, 2017,7(1):147.