炭基菌剂高效还原土壤Cr(VI)的电子传递路径

张方; 陈友媛; 戴立前; 乔雪琴; 颜雨欣; 杨阳; 刘乐成

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (6) : 3180-3189.

土壤污染与控制

炭基菌剂高效还原土壤Cr(VI)的电子传递路径

张方¹, 陈友媛^1,2, 戴立前³, 乔雪琴¹, 颜雨欣¹, 杨阳¹, 刘乐成^1,2

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Electron transfer pathways for efficient reduction of soil Cr(VI) by biochar-based microbial agents

ZHANG Fang¹, CHEN You-yuan^1,2, DAI Li-qian³, QIAO Xue-qin¹, YAN Yu-xin¹, YANG Yang¹, LIU Le-cheng^1,2

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摘要

为了阐明生物炭和典型功能微生物希瓦氏菌MR-1(Shewanella oneidensis MR-1)在电子传递中的协同作用,以Cr(VI)污染土壤为载体,开展了电化学,动力学,电子传递路径识别等研究.结果表明,炭基菌剂对Cr(VI)污染土壤具有较好的修复效果,修复机制以MR-1对Cr(VI)的生物还原为主,符合双过程动力学模型.25mg/kg Cr(VI)含量,5%炭基菌剂投加量,30%土壤含水率时,炭基菌剂还原土壤Cr(VI)的效率最高,可达96.30%.吸附法炭基菌剂还原Cr(VI)迅速,但持续性能差;包埋法炭基菌剂还原Cr(VI)慢,但长期稳定发挥作用.生物炭作为微生物载体促进MR-1生长的同时,将MR-1表面的电子经由碳骨架传递至Cr(VI),提高了Cr(VI)还原效率.3个循环后炭基菌剂对Cr(VI)的还原率仍可达到60.44%,表明炭基菌剂在长期高效修复铬污染土壤方面有一定的应用前景.

Abstract

This study systematically investigated the synergistic interactions between biochar and the model electroactive microorganism Shewanella oneidensis MR-1 in electron transfer processes through comprehensive electrochemical analyses, kinetic modeling, and electron pathway characterization using chromium(VI)-contaminated soil as the experimental matrix. The biochar-based microbial agents demonstrated effective Cr(VI) bioremediation, with biological reduction mediated by MR-1identified as the predominant mechanism following dual-process kinetics. Optimal remediation performance (96.30% Cr(VI) reduction efficiency) was achieved under conditions of 25mg/kg Cr(VI) contamination, 5% (w/w) biochar-based microbial agents dosage, and 30% soil moisture content. Comparative analysis revealed distinct temporal remediation patterns: adsorption-based biochar-microbial composites exhibited rapid initial Cr(VI) sequestration but limited long-term stability, whereas encapsulation-based formulations showed gradual but sustained reduction capacity. Mechanistic studies demonstrated that biochar functioned as an effective microbial carrier, simultaneously enhancing MR-1proliferation and facilitating extracellular electron transfer from microbial cells to Cr(VI) contaminants through its conductive carbon matrix. Notably, the immobilized system maintained 60.44% reduction efficiency after three operational cycles, highlighting its potential for sustainable in situ remediation of chromium-contaminated soils.

导出引用

张方, 陈友媛, 戴立前, 乔雪琴, 颜雨欣, 杨阳, 刘乐成. 炭基菌剂高效还原土壤Cr(VI)的电子传递路径[J]. 中国环境科学. 2025, 45(6): 3180-3189

ZHANG Fang, CHEN You-yuan, DAI Li-qian, QIAO Xue-qin, YAN Yu-xin, YANG Yang, LIU Le-cheng. Electron transfer pathways for efficient reduction of soil Cr(VI) by biochar-based microbial agents[J]. China Environmental Science. 2025, 45(6): 3180-3189

中图分类号： X53

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