马尾松林土壤磷赋存形态及其对含phoD基因解磷细菌群落的影响

李绍隆; 屠豪阳; 阮艳萍; 赵亮; 林敦梅

PDF(833 KB)

中国环境科学 ›› 2025, Vol. 45 ›› Issue (6) : 3190-3198.

土壤污染与控制

马尾松林土壤磷赋存形态及其对含phoD基因解磷细菌群落的影响

李绍隆, 屠豪阳, 阮艳萍, 赵亮, 林敦梅

作者信息 +

The phosphorus forms in the soil of Pinus massoniana forest and their impact on the community of phosphorus-decomposing bacteria harboring phoD genes

LI Shao-long, TU Hao-yang, RUAN Yan-ping, ZHAO Liang, LIN Dun-mei

Author information +

文章历史 +

摘要

本研究测定了重庆主城区内8个代表性马尾松林样地表层土壤(0~10cm)不同赋存形态磷元素含量,并基于高通量测序技术研究了含碱性磷酸酶(phoD)基因的细菌群落特征.结果表明,研究区马尾松林表层土磷含量较低,冬季和夏季土壤总磷(TP)含量均值分别为192.787,169.512mg/kg.其中无机磷形态含量分布为闭蓄态磷(O-P)>铁结合磷(Fe-P)>铝结合磷(Al-P)>钙结合磷(Ca-P)>可交换态磷(Ex-P),并呈现冬高夏低的季节变化格局.含phoD基因细菌群落优势门为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和浮霉菌门(Planctomycetes),平均相对丰度达到96%.相关分析表明含phoD基因细菌多样性与Al-P之间存在显著负相关关系(P<0.05),与土壤pH值存在显著正相关关系(P<0.05).冗余分析表明Al-P和pH值是影响含phoD基因细菌群落结构的最主要因素.综上所述,重庆主城区马尾松林表层土普遍缺磷且磷含量受到季节变化的显著影响,磷赋存形态影响含phoD基因细菌群落结构及多样性.

Abstract

In this study, we measured the contents of P in various forms in the topsoil (0~10cm) of 8representative pine (Pinus massoniana) forests in the main urban area of Chongqing. We used high-throughput sequencing technology to investigate the community characteristics of the phoD-harboring bacteria. The results showed that the surface soils of Pinus massoniana forests in the study area had relatively low phosphorus levels, with average total phosphorus (TP) contents of 192.787mg/kg in winter and 169.512mg/kg in summer. Among the inorganic phosphorus fractions, the content distribution followed the order: occluded phosphorus (O-P) > iron-bound phosphorus (Fe-P) > aluminum-bound phosphorus (Al-P) > calcium-bound phosphorus (Ca-P) > exchangeable phosphorus (Ex-P), showing a seasonal pattern of higher levels in winter and lower levels in summer. The dominant phyla of phoD-harboring bacteria in the soil were Proteobacteria, Actinobacteria, and Planctomycetes, collectively accounting for 96% of the average relative abundance. Correlation analysis showed a significant negative correlation (P<0.05) between the diversity of phoD-harboring bacteria and Al-P, as well as a significant positive correlation (P<0.05) with soil pH. Redundancy analysis indicated that Al-P and pH were the most important factors influencing the community structure of phoD-harboring bacteria. In conclusion, the topsoil of pine forests in the main urban area of Chongqing is generally P-deficient, and the P content is significantly influenced by season. The forms of P occurrence affect the community structure and diversity of phoD-harboring bacteria.

导出引用

李绍隆, 屠豪阳, 阮艳萍, 赵亮, 林敦梅. 马尾松林土壤磷赋存形态及其对含phoD基因解磷细菌群落的影响[J]. 中国环境科学. 2025, 45(6): 3190-3198

LI Shao-long, TU Hao-yang, RUAN Yan-ping, ZHAO Liang, LIN Dun-mei. The phosphorus forms in the soil of Pinus massoniana forest and their impact on the community of phosphorus-decomposing bacteria harboring phoD genes[J]. China Environmental Science. 2025, 45(6): 3190-3198

中图分类号： X171

参考文献

[1] 崔瑶,辜夕容,严宁珍,等.缙云山4种林分土壤无机磷与活性铝的含量及分布[J]. 生态学报, 2022,42(5):1727-1738.Cui Y, Gu X R, Yan N Z, et al. Content and distribution of inorganic phosphorus and labile aluminum in soil of four forest types in Jinyun Mountain [J]. Acta Ecologica Sinica, 2022,42(5):1727-1738.
[2] Luo S P, He B H, Zeng Q P, et al. Effects of seasonal variation on soil microbial community structure and enzyme activity in a Masson pine forest in Southwest China [J]. Journal of Mountain Science, 2020, 17(6):1398-1409.
[3] Hu Y, Xia Y, Sun Q, et al. Effects of long-term fertilization on phoD-harboring bacterial community in Karst soils [J]. Science of the Total Environment, 2018,628-629(575):53-63.
[4] Spohn M, Kuzyakov Y. Distribution of microbial- and root-derived phosphatase activities in the rhizosphere depending on P availability and C allocation-Coupling soil zymography with ¹⁴C imaging [J]. Soil Biology & Biochemistry, 2013,67:106-113.
[5] Krämer S. Acid and alkaline phosphatase dynamics and their relationship to soil microclimate in a semiarid woodland [J]. Soil Biology & Biochemistry, 2000,32(2):179-188.
[6] Tazisong I A, Senwo Z N, He Z. Phosphatase hydrolysis of organic phosphorus compounds [J]. Advances in Enzyme Research, 2015,3(2): 39-51.
[7] Zheng M M, Wang C, Li W X, et al. Changes of acid and alkaline phosphatase activities in long-term chemical fertilization are driven by the similar soil properties and associated microbial community composition in acidic soil [J]. European Journal of Soil Biology, 2021,104(6):103312.
[8] Zheng M M, Wang C, Li W X, et al. Soil nutrients drive function and composition of phoC-harboring bacterial community in acidic soils of Southern China [J]. Front Microbiol., 2019,10:2654.
[9] Bergkemper F, Schöler A, Engel M, et al. Phosphorus depletion in forest soils shapes bacterial communities towards phosphorus recycling systems [J]. Environmental Microbiology, 2016,186(9): 1988-2000.
[10] Li J B, Xie T, Zhu H, et al. Alkaline phosphatase activity mediates soil organic phosphorus mineralization in a subalpine forest ecosystem [J]. Geoderma, 2021,404(68):115376.
[11] Lagos L M, Acuña J J, Maruyama F, et al. Effect of phosphorus addition on total and alkaline phosphomonoesterase-harboring bacterial populations in ryegrass rhizosphere microsites [J]. Biology and Fertility of Soils, 2016,52(7):1007-1019.
[12] Luo G W, Ling N, Nannipieri P, et al. Long-term fertilisation regimes affect the composition of the alkaline phosphomonoesterase encoding microbial community of a vertisol and its derivative soil fractions [J]. Biology and Fertility of Soils, 2017,53(4):375-388.
[13] Fraser T, Lynch D H, Entz M H, et al. Linking alkaline phosphatase activity with bacterial phoD gene abundance in soil from a long-term management trial [J]. Geoderma, 2015,257-258(9):115-122.
[14] Wei X M, Hu Y J, Cai G, et al. Organic phosphorus availability shapes the diversity of phoD-harboring bacteria in agricultural soil [J]. Soil Biology & Biochemistry, 2021,161(5):108364.
[15] Wan W J, He D L, Li X, et al. Adaptation of phoD-harboring bacteria to broader environmental gradients at high elevations than at low elevations in the Shennongjia primeval forest [J]. Geoderma, 2021, 401(56):115210.
[16] Wang M, Wu Y, Zhao J, et al. Long-term fertilization lowers the alkaline phosphatase activity by impacting the phoD-harboring bacterial community in rice-winter wheat rotation system [J]. Science of the Total Environment, 2022,821(3):153406.
[17] 薛沛沛,齐代华,陈道静,等.马尾松林改造成香樟林过程中生态系统服务功能评估[J]. 四川林业科技, 2019,40(1):30-34.Xue P P, Qi D H, Chen D J, et al. Forest ecosystem service and its evaluation in the transforming process from Pinus massoniana forest into Cinnamomum camphora forest [J]. Journal of Sichuan Forestry Science and Technology, 2019,40(1):30-34.
[18] Cui N, Chen X, Liu Y, et al. Shrub and herb diversity at different ages of Pinus massoniana plantation [J]. Acta Ecologica Sinica, 2014, 34(15):4313-4323.
[19] 王轶浩,陈展,周建岗,等.重庆酸雨区马尾松纯林改造对土壤酸化特征及团聚体稳定性的影响[J]. 生态学报, 2021,41(13):5184- 5194.Wang Y H, Chen Z, Zhou J G, et al. Effects of transformation of Masson pine forest on characteristics of soil acidification and aggregate stability within polluted areas of Chongqing. [J] Acta Ecologica Sinica, 2021,41(13):5184-5194.
[20] 陈历睿,林佳妮,沈蓉,等.三峡库区马尾松林土壤真菌群落特征及影响因素[J]. 应用生态学报, 2023,33(9):2397-2404.Chen L R, Lin J N, Shen R N, et al. Characteristic of soil fungal community and the influencing factors of Pinus massoniana forests in the Three Gorges Reservoir Region [J]. Chinese Journal of Applied Ecology, 2022,33(9): 2397-2404.
[21] Chang S C, Jackson M L W. Fractionation of soil phosphorus [J]. Soil Science, 1957,84(9):133–144.
[22] Friedman J, Alm E J. Inferring correlation networks from genomic survey data [J]. PLoS Computational Biology, 2012,8(9):e1002687.
[23] Lai J S, Zou Y, Zhang J L, et al. Generalizing hierarchical and variation partitioning in multiple regression and canonical analyses using the rdacca.hp R package [J]. Methods in Ecology and Evolution, 2022,13(4):782-788.
[24] 王树力.不同经营类型红松林对汤旺河流域土壤性质的影响[J]. 水土保持学报, 2006,(2):90-93.Wang S L. Effects of different management types of Pinus koraiensis forest on soil properties around Tangwang river [J] Journal of Soil and Water Conservation, 2006,(2):90-93.
[25] 冼应男,陈婵,方晰,等.湘中丘陵区4种植被类型土壤全磷、有效磷含量的变异特征[J]. 中南林业科技大学学报, 2019,39(5):79- 88.Xian Y N, Chen C, Fang X, et al. Variation characteristics of soil total phosphorus and effective phosphorus concentrations in four vegetation type soils in the central hilly area of Hunan Province, China [J]. Journal of Central South University of Forestry & Technology, 2019, 39(5):79-88.
[26] 简尊吉,倪妍妍,徐瑾,等.中国马尾松林土壤肥力特征[J]. 生态学报, 2021,41(13):5279-5288.Jian Z J, Ni Y Y, Xu J, et al. Soil fertility in the Pinus massoniana forests of China [J]. Acta Ecologica Sinica, 2021,41(13):5279-5288.
[27] Huang X, Foster G D, Honeychuck R V, et al. The maximum of phosphate adsorption at pH 4.0: why it appears on aluminum oxides but not on iron oxides? [J]. Langmuir, 2009,25(8):4450-4461.
[28] Li J S, Wu B Y, Zhang D D, et al. Elevational variation in soil phosphorus pools and controlling factors in alpine areas of Southwest China [J]. Geoderma, 2023,431:116361.
[29] 陆晓辉,丁贵杰.马尾松人工纯林凋落松针数量及基质质量动态[J]. 生态学报, 2017,37(22):7568-7575.Lu X H, Ding G J. Dynamics and quality of leaf litter fall in pure Pinus massoniana plantations [J]. Acta Ecologica Sinica, 2017,37(22): 7568-7575.
[30] Yu Z, Guoyi Z, Liu L, et al. Natural forests promote phosphorus retention in soil [J]. Global Change Biology, 2022,28(4):1678-1689.
[31] Xu Z M, Adusei-Fosu K, Wang H L, et al. Interactive effects of biochar and phosphorus fertilizer on PhoD-harboring bacteria and phosphorus dynamics in Moso Bamboo forest soil [J]. Journal of Soil Science and Plant Nutrition, 2024,24:7010–7023.
[32] 滕泽栋,李敏,朱静,等.野鸭湖湿地芦苇根际微生物多样性与磷素形态关系[J]. 环境科学, 2017,38(11):4589-4597.Teng Z D, Li M, Zhu J, et al. Effects of soil microbial diversity on the phosphate fraction in the rhizosphere of phragmites communis in the Yeyahu wetland in Beijing, China [J]. Environmental Science, 2017, 38(11):4589-4597.
[33] 佘晨兴,林洪,苏玉萍,等.闽江口-平潭海域有机解磷菌多样性及群落特征[J]. 应用生态学报, 2021,32(5):1863-1872.She C X, Lin H, Su Y P, et al. Diversity and community characteristics of organic phosphate-mineralizing bacteria in the sea area between Minjiang Estuary to Pingtan [J]. Chinese Journal of Applied Ecology, 2021,32(5):1863-1872.
[34] Gao Y, Tariq A, Zeng F, et al. Fractions of soil phosphorus mediated by rhizospheric phoD-harbouring bacteria of deep‐rooted desert species are determined by fine-root traits [J]. Functional Ecology, 2024,38(10):2300-2315.
[35] Bergkemper F, Scholer A, Engel M, et al. Phosphorus depletion in forest soils shapes bacterial communities towards phosphorus recycling systems [J]. Environmental Microbiology, 2016,18(6):1988- 2000.
[36] Zhang G, Bai J, Zhai Y, et al. Microbial diversity and functions in saline soils: A review from a biogeochemical perspective [J]. Journal of Advanced Research, 2024,59:129-140.
[37] Du X, Liu N, Yan B, et al. Proximity-based defensive mutualism between Streptomyces and Mesorhizobium by sharing and sequestering iron [J]. ISME Journal, 2024,18(1):wrad041.
[38] Pauleta S R, Dell'acqua S, Moura I. Nitrous oxide reductase [J]. Coordination Chemistry Reviews, 2013,257(2):332-349.
[39] Fierer N. Embracing the unknown: disentangling the complexities of the soil microbiome [J]. Nature Reviews Microbiology, 2017,15(10): 579-590.
[40] Zhou Z, Wang C, Luo Y. Meta-analysis of the impacts of global change factors on soil microbial diversity and functionality [J]. Nature Communications, 2020,11(1):3072.
[41] Eder S, Shi L, Jensen K, et al. A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD [J]. Microbiology, 1996,142(8):2041-2047.
[42] Tan H, Barret M, Mooij M J, et al. Long-term phosphorus fertilisation increased the diversity of the total bacterial community and the phoD phosphorus mineraliser group in pasture soils [J]. Biology and Fertility of Soils, 2013,49(6):661-672.
[43] Gao Y, Tariq A, Zeng F, et al. Soil microbial functional profiles of P-cycling reveal drought-induced constraints on P-transformation in a hyper-arid desert ecosystem [J]. Science of the Total Environment, 2024,925:171767.
[44] 贺根和.铝胁迫对红壤微生物的生态毒理效应及微生物的耐铝特性研究[D]. 南京:南京大学, 2012.He G H. Studies on microbial ecological and toxicological effects by aluminum stress and microbial aluminum-tolerance characteristics in acidic red soils. [D]. Nanjing: Nanjing University, 2012.
[45] Philippot L, Chenu C, Kappler A, et al. The interplay between microbial communities and soil properties [J]. Nature Reviews Microbiology, 2024,22(4):226-239.
[46] Šimek M, Cooper J E. The influence of soil pH on denitrification: progress towards the understanding of this interaction over the last 50years [J]. European Journal of Soil Science, 2002,53(3):345-354.
[47] Nannipieri P, Giagnoni L, Landi L, et al. Role of Phosphatase Enzymes in Soil [M]. Berlin: Springer Berlin Heidelberg, 2011:215- 243.
[48] Odum E P. The strategy of ecosystem development: an understanding of ecological succession provides a basis for resolving man’s conflict with nature [J]. Science, 1969,164(3877):262-270.
[49] Jiang M, Crous K Y, Carrillo Y, et al. Microbial competition for phosphorus limits the CO₂ response of a mature forest [J]. Nature, 2024,630(8017):660-665.