达里湖夏季浮游细菌群落结构及其氮、磷代谢功能

金宇琦; 李文宝; 史玉娇; 张博尧; 杜蕾; 郭鑫

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (6) : 3268-3279.

环境生态

达里湖夏季浮游细菌群落结构及其氮、磷代谢功能

金宇琦¹, 李文宝^1,2, 史玉娇¹, 张博尧¹, 杜蕾¹, 郭鑫¹

作者信息 +

Structure of bacterioplankton community and its function of nitrogen and phosphorus metabolism in Lake Dali in summer

JIN Yu-qi¹, LI Wen-bao^1,2, SHI Yu-jiao¹, ZHANG Bo-yao¹, DU Lei¹, GUO Xin¹

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摘要

为分析湖泊不同水深中细菌群落结构对N、P代谢功能丰度的响应,本文以达里湖为研究对象,通过高通量测序技术,开展表、中、底水体中细菌群落结构及N、P相关代谢功能丰度空间分布及相关性特征分析.结果显示,随着水体理化性质出现变化,表、中、底3层水体中优势细菌群落构成差异明显.基于生态网络,在度值和丰度值筛选标准下对前5个优势菌门下的优势菌属及重要菌属进行分析,达里湖不同深度水体中优势菌属发生明显转变,如表、中层水中有3个菌属出现变化,而底层水则有2个菌属出现改变.相对地,表层水中CL500-29_marine_group和栖热菌属,中层水中聚球菌属和norank_o__NB1-n以及底层水中聚球菌属、norank_o__NB1-n和norank_f__CK06-06-Mud-MAS4B-21在两种筛选标准下保持一致.进一步对细菌群落的功能组成进行PICRUSt2预测,得到6种初级功能及12种次级代谢功能.特别地,虽然假单胞菌属、副球菌属及聚球菌属均与N、P代谢功能丰度变化呈现显著正相关,但与不同形态N、P含量之间相关性却存在差异,如假单胞菌属与N、P含量变化呈正相关,副球菌属与N、P含量呈负相关,而聚球菌属则与N元素呈负相关,与P元素呈正相关.整体上,水深变化引起的N、P元素含量差异对N、P代谢功能及优势菌属具有显著影响.

Abstract

In order to analyse the response of bacterial community structure to the abundance of nitrogen (N) and phosphorus (P) metabolic functions in different water depths of lakes, this paper presents a characterization of the spatial distribution and correlation of bacterial community structure and the abundance of N and P related metabolic functions in the surface, middle and bottom waterbodies by high-throughput sequencing technology, with Lake Dali as the object of the study. The results showed that the composition of the dominant bacterial communities in the surface, middle and bottom water layers varied significantly with the changes in the physical and chemical properties of the water. Based on the ecological network, the dominant and important genera under the first five dominant phyla were analysed under the screening criteria of degree and abundance values, and the dominant genera in the different depths of Lake Dali were significantly changed, such as three genera in the surface and middle water, and two genera in the bottom water. Comparatively, CL500-29_marine_group and Thermus in the surface water, Synechococcus and norank_o__NB1-n in the middle water, and Synechococcus、norank_o__NB1-n、norank_f__CK06-06-Mud-MAS4B-21 in the bottom water, remained consistent with the two screening criteria. Further PICRUSt2 prediction of the functional composition of the bacterial community yielded 6 primary functions and 12 secondary metabolic functions. In particular, although Pseudomonas, Paracoccus and Synechococcus showed significant positive correlation with the abundance of N and P metabolic functions, the correlation with the N and P content of different forms was different, such as Pseudomonas showed positive correlation with the change of N and P content, Paracoccus showed negative correlation with the N and P content, and Synechococcus showed negative correlation with N and positive correlation with P elements. Overall, the differences in N and P contents caused by changes in water depth had significant effects on N and P metabolism and the dominant genera.

导出引用

金宇琦, 李文宝, 史玉娇, 张博尧, 杜蕾, 郭鑫. 达里湖夏季浮游细菌群落结构及其氮、磷代谢功能[J]. 中国环境科学. 2025, 45(6): 3268-3279

JIN Yu-qi, LI Wen-bao, SHI Yu-jiao, ZHANG Bo-yao, DU Lei, GUO Xin. Structure of bacterioplankton community and its function of nitrogen and phosphorus metabolism in Lake Dali in summer[J]. China Environmental Science. 2025, 45(6): 3268-3279

中图分类号： X172

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