固定化菌剂对PAEs-Cd复合污染土壤的修复作用

王凡; 李文杰; 毛鹏飞; 高彦征

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (6) : 3394-3401.

农业与农村新污染物

固定化菌剂对PAEs-Cd复合污染土壤的修复作用

王凡, 李文杰, 毛鹏飞, 高彦征

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Remediation of contaminated soil with PAEs and Cd using an immobilized microbial agent

WANG Fan, LI Wen-jie, MAO Peng-fei, GAO Yan-zheng

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摘要

针对广西农田土壤中存在的邻苯二甲酸酯(PAEs)和镉(Cd)复合污染问题,提出了固定化功能菌剂修复复合污染农田土壤的思路.由Gordonia sp.、Rhodococcus sp.、Bacillus sp.等3种功能菌株构建出具备耐Cd和降解PAEs性能的复合菌群,以巯基改性蒙脱石-生物炭复合材料为固定化载体,制备了固定化菌剂,优化了其制备条件,揭示了菌剂施用对PAEs-Cd复合污染土壤的协同修复效能,探究功能菌剂的作用机制.结果表明,5d内复合菌群对6种优控PAEs的总降解率达92.7%,载体材料对Cd饱和吸附量达15.2mg/kg;菌剂最优固定化条件为30℃、菌料比1:20(V/M)下固定1d.制备的固定化菌剂5d内对ΣPAEs降解率达95.4%;以1%施用量投加固定化菌剂至PAEs-Cd复合污染土壤中,50d后土壤中PAEs消减率达54.14%,可交换态Cd减少37.06%,菌剂对PAEs-Cd复合污染有良好的协同修复效能.

Abstract

To address the co-contamination of phthalic acid esters (PAEs) and cadmium (Cd) in agricultural soils of Guangxi province, a novel approach using immobilized functional microbial agent has been proposed. A composite microbial consortium, composed of three functional bacterial strains including Gordonia sp., Rhodococcus sp., and Bacillus sp., was developed with the ability to tolerate Cd and degrade PAEs. The microbial agent was immobilized on a thiol-modified montmorillonite-biochar composite carrier with optimized preparation conditions to enhance their remediation capabilities. The synergistic remediation efficacy of the agent on PAEs-Cd co-contaminated soils and the underlying mechanisms were elucidated. The results demonstrated that the composite microbial consortium achieved a degradation rate of 92.7% for total PAEs within 5days, while the carrier material exhibited a Cd saturation adsorption capacity of 15.2mg/kg. The optimal immobilization conditions were determined to be 30℃, with a bacteria-to-carrier ratio of 1:20 (V/M) for 1day. Under these conditions, the immobilized microbial agent achieved a degradation rate of 95.4% for ΣPAEs within 5days. When applied at a dosage of 1% to PAEs-Cd co-contaminated soils, the immobilized microbial agent resulted in 54.14% PAEs elimination and 37.06% decrease of exchangeable Cd after 50days. The immobilized microbial agent exhibited favorable synergistic remediation efficacy for PAEs-Cd co-contamination. The research findings provided a theoretical basis for the remediation of PAEs-Cd co-contamination in farmland soil of Guangxi and filled the theoretical gap in the control and remediation of PAEs-Cd co-contamination.

导出引用

王凡, 李文杰, 毛鹏飞, 高彦征. 固定化菌剂对PAEs-Cd复合污染土壤的修复作用[J]. 中国环境科学. 2025, 45(6): 3394-3401

WANG Fan, LI Wen-jie, MAO Peng-fei, GAO Yan-zheng. Remediation of contaminated soil with PAEs and Cd using an immobilized microbial agent[J]. China Environmental Science. 2025, 45(6): 3394-3401

中图分类号： X53

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