厌氧铁氨氧化功能微生物的驯化与AQDS强化作用

朱丹阳; 刁思圆; 宫徽; 戴晓虎

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (7) : 3735-3741.

水污染与控制

厌氧铁氨氧化功能微生物的驯化与AQDS强化作用

朱丹阳¹, 刁思圆², 宫徽¹, 戴晓虎¹

作者信息 +

Enrichment of Feammox functional microorganisms and the strengthening effect of AQDS.

ZHU Dan-yang¹, DIAO Si-yuan², GONG Hui¹, DAI Xiao-hu¹

Author information +

文章历史 +

摘要

利用厌氧消化污泥,通过添加含氨氮和Fe (III)的配水富集厌氧铁氨氧化(Feammox)功能微生物,并评估其长期脱氮性能.结果表明,第66d出现Feammox活性,并在后续批次中保持稳定;Feammox潜在功能菌Geobacter和Anaeromyxobacter的相对丰度分别增加了457倍和11.6倍.宏基因组分析显示,3条与亚硝氮转化相关的途径功能基因总相对丰度显著提升,包括氨氮氧化为亚硝氮(amo、hao),亚硝氮反硝化为氮气(nirSK、norB、nosCZ)和亚硝氮还原为铵(nirABD、nrfAH).添加电子穿梭体蒽醌-2,6-二磺酸(AQDS)后可促进细胞外电子转移,氨氮去除率提高了17.8%.

Abstract

Feammox functional microorganisms were enriched from anaerobic digestion sludge with NH₄⁺-N and Fe(III) supplementation and their long-term nitrogen removal performance was evaluated. Feammox activity was detected on day 66and remained stable in subsequent batches. The relative abundances of the potential Feammox functional bacteria Geobacter and Anaeromyxobacter increased by 457-fold and 11.6-fold, respectively. Metagenomic analysis revealed a significant increase in the total relative abundance of functional genes associated with three nitrite transformation pathways: ammonia oxidation to nitrite (amo, hao), nitrite denitrification to nitrogen gas (nirSK, norB, nosCZ), and nitrite reduction to ammonium (nirABD, nrfAH). The addition of the electron shuttle AQDS promoted extracellular electron transfer and enhanced the ammonia removal efficiency by 17.8%.

导出引用

朱丹阳, 刁思圆, 宫徽, 戴晓虎. 厌氧铁氨氧化功能微生物的驯化与AQDS强化作用[J]. 中国环境科学. 2025, 45(7): 3735-3741

ZHU Dan-yang, DIAO Si-yuan, GONG Hui, DAI Xiao-hu. Enrichment of Feammox functional microorganisms and the strengthening effect of AQDS.[J]. China Environmental Science. 2025, 45(7): 3735-3741

中图分类号： X703.1

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