流式细胞仪快速计量饮用水中微塑料数量

步海鑫; 邵森林; 李凯; 文刚; 黄廷林

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (7) : 3807-3813.

水污染与控制

流式细胞仪快速计量饮用水中微塑料数量

步海鑫¹, 邵森林², 李凯¹, 文刚¹, 黄廷林¹

作者信息 +

Rapid measurement of microplastics in drinking water by flow cytometry.

BU Hai-xin¹, SHAO Sen-lin², LI Kai¹, WEN Gang¹, HUANG Ting-lin¹

Author information +

文章历史 +

摘要

针对缺乏不需要视觉分类或预处理的快速监测技术问题,基于流式细胞仪开发可快速测定饮用水中微塑料数目的新方法.结果表明,选用尼罗红染料(NR),以甲醇作为的其溶剂、染料浓度选用10μg/mL、染色10min后,再采用流式细胞仪进行测定,可以实现水中微塑料的定量分析,单个样品的测试时间可以缩短到14min以内.应用该方法测定了商品瓶装水和超滤膜进出水的微塑料,结果表明商品瓶装水中微塑料浓度在(0.46~1.52)×10⁵个/L.新使用的聚偏氟乙烯(PVDF)超滤膜仅在过滤初期(第1d)存在较高的微塑料释放风险,在长期运行中微塑料的释放可以忽略,另外其对进水中微塑料可以实现99.99%的高效去除.

Abstract

Developing a new method based on flow cytometry for rapid determination of the number of microplastics in drinking water, in response to the lack of rapid monitoring techniques that do not require visual sorting or pre-processing. The results showed that the use of Nile red dye (NR), methanol as the solvent, a dye concentration of 10μg/mL, a dyeing time of 10minutes, and flow cytometry for the quantitative analysis of microplastics in water, could reduce the testing time of a single sample to less than 14minutes. This method was used to analyse microplastics in influent and effluent water from a commercial bottled water plant and an ultrafiltration membrane. The results showed that the microplastic concentration in commercial bottled water was in the range (0.46~1.52)×10⁵ particles/L. The newly used polyvinylidene fluoride (PVDF) ultrafiltration membrane posed a higher risk of releasing microplastics only during the initial filtration stage (1d), and the release of microplastics during long-term operation could be disregarded. This new membrane's microplastic release could be analysed with 99.99% efficiency and effectiveness. Additionally, it can efficiently and effectively remove 99.99% of microplastics from feed water.

导出引用

步海鑫, 邵森林, 李凯, 文刚, 黄廷林. 流式细胞仪快速计量饮用水中微塑料数量[J]. 中国环境科学. 2025, 45(7): 3807-3813

BU Hai-xin, SHAO Sen-lin, LI Kai, WEN Gang, HUANG Ting-lin. Rapid measurement of microplastics in drinking water by flow cytometry.[J]. China Environmental Science. 2025, 45(7): 3807-3813

中图分类号： X52

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