徒骇河典型断面DOM特征及重金属结合能力

王珊珊; 桑冬玲; 刘强; 姚昕; 孙赵利; 张家政; 张晨; 李婷婷

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (8) : 4398-4409.

水污染与控制

徒骇河典型断面DOM特征及重金属结合能力

王珊珊¹, 桑冬玲¹, 刘强², 姚昕¹, 孙赵利¹, 张家政¹, 张晨¹, 李婷婷¹

作者信息 +

Characterization of dissolved organic matter and its heavy metal binding potential at typical cross-sections of the Tuhai River

WANG Shan-shan¹, SANG Dong-ling¹, LIU Qiang², YAO Xin¹, SUN Zhao-li¹, ZHANG Jia-zheng¹, ZHANG Chen¹, LI Ting-ting¹

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文章历史 +

摘要

选取徒骇河聊城河段的2个典型市控断面——李凤桃与老南镇断面进行表层水样采集,利用紫外可见吸收光谱和三维荧光光谱等多种光谱分析手段,并结合超滤技术及荧光淬灭实验,探究不同季节和不同分子量(<0.7μm、0.7μm~1kDa、<1kDa)DOM的特征差异及其与重金属(Cu²⁺)相互作用的机制.结果表明,李凤桃SUVA₂₅₄值(0.33±0.01)显著高于老南镇(0.30±0.01)(P<0.05),表明老南镇分布着更多的小分子和非芳香性物质.研究基于EEM-PARAFAC共识别出了5个组分,包括3个类腐殖质组分(C1、C2、C4)和2个类蛋白质组分(C3、C5),其中类腐殖质组分在两断面的DOM中占主导地位.老南镇DOM总荧光强度(F_max)高于李凤桃,且两断面溶解性有机碳(DOC)主要储存在低分子量DOM(<1kDa)中.在DOM与Cu²⁺的结合过程中,二维相关光谱(2D-COS)表明老南镇和李凤桃断面DOM类腐殖质优先与Cu²⁺络合;分馏后,两断面DOM的结合序列发生了变化(类蛋白质优先与Cu²⁺发生络合).此外,在两断面DOM中,高分子量组分(除C1和C4外)具有较高的金属结合能力(logK_Cu:(3.08±0.08)~(3.62±0.02)).

Abstract

Surface water samples were collected from two municipally monitored sections of the Tuhai River in Liaocheng—Li Fengtao and Lao Nanzhen—and analyzed using a combination of spectroscopic techniques, including ultraviolet-visible (UV-Vis) absorption and three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy, supplemented by ultrafiltration fractionation and fluorescence-quenching experiments to comprehensively characterize dissolved organic matter (DOM). The study investigated seasonal variations in DOM composition across three molecular weight fractions (<0.7μm, 0.7μm~1kDa, and<1kDa) and explored the underlying mechanisms of DOM-Cu²⁺ complexation. The specific ultraviolet absorbance at 254nm (SUVA254) was significantly higher at Li Fengtao (0.33 ± 0.01) than at Lao Nanzhen (0.30 ± 0.01) (P<0.05), suggesting a higher proportion of smaller, non-aromatic molecules at the latter site. Parallel factor analysis (PARAFAC) of EEM data identified five fluorescent components, including three humic-like (C1, C2, C4) and two protein-like (C3, C5) substances, with humic-like constituents dominating at both locations. Total fluorescence intensity (F_max) was higher at Lao Nanzhen, and dissolved organic carbon (DOC) was primarily concentrated in the <1kDa fraction at both sites. Two-dimensional correlation spectroscopy (2D-COS) analysis demonstrated that humic-like components preferentially bound to Cu²⁺ in the unfractionated DOM samples from both locations. However, after ultrafiltration fractionation, the binding sequence shifted, with protein-like components becoming the dominant Cu²⁺-binding constituents. Furthermore, high-molecular-weight fractions (excluding C1and C4) from both sites exhibited relatively strong metal-binding capacities, as evidenced by their conditional stability constants (logK_Cu:(3.08 ± 0.08) to (3.62 ± 0.02)).

导出引用

王珊珊, 桑冬玲, 刘强, 姚昕, 孙赵利, 张家政, 张晨, 李婷婷. 徒骇河典型断面DOM特征及重金属结合能力[J]. 中国环境科学. 2025, 45(8): 4398-4409

WANG Shan-shan, SANG Dong-ling, LIU Qiang, YAO Xin, SUN Zhao-li, ZHANG Jia-zheng, ZHANG Chen, LI Ting-ting. Characterization of dissolved organic matter and its heavy metal binding potential at typical cross-sections of the Tuhai River[J]. China Environmental Science. 2025, 45(8): 4398-4409

中图分类号： X522

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