以地下水中硝酸盐及检出率较高的氧氟沙星(OFL)作为目标污染物,基于零价铁(ZVI)和黄铁矿(FeS2)双介质反应体系,研究了铁反硝化功能微生物种群(M)的接种量对ZVI/FeS2去除硝酸盐和OFL的去除效果;探究了主要水化学参数,硝酸盐和OFL浓度对耦合体系(ZVI/FeS2＋M)的影响规律,并探讨了微生物强化ZVI/FeS2去除水体中硝酸盐和氧氟沙星的机制.结果表明,铁反硝化功能微生物能明显强化ZVI/FeS2对硝酸盐和OFL的去除,当ZVI/FeS2的质量比例和总投加量分别为2:1和16g/L,微生物(M)接种量为5.81g/L, ZVI/FeS2＋M耦合体系中的硝酸盐在168h内几乎被完全去除,而OFL的去除率也高达90.44%.同时, ZVI/FeS2+M体系在不同温度(10~30℃),硝酸盐浓度(25~150mg/L), OFL浓度(500ng/L~200ug/L)和共存离子的存在下均表现出很好的适应性.微生物的介入大大延缓了ZVI表面的钝化氧化现象,同时加速了ZVI/FeS2的溶矿作用,其溶矿过程中生成的FeS, Fe2+和S2-均有利于污染物的去除.循环6次后的ZVI/FeS2+M耦合体系对硝酸盐和OFL的去除率分别为73.80%和79.25%,仍高于ZVI/FeS2单批次的去除效果(62.73%和60.60%).
Abstract
In this study, nitrate and ofloxacin(OFL) with high detection rate in groundwater were taken as target pollutants, and the effects of iron denitrification microorganism(M) with different inoculation dosage on the removal of nitrate and OFL by the dual materials of micro ZVI/FeS2 system was investigated. Also, the influence of the major water chemical parameters, nitrate and OFL concentrations on the removal of nitrate and OFL in the coupled system(ZVI/FeS2+M) was studied. In addition, the enhanced mechanisms for removing nitrate and OFL in the coupled system were explored. The results showed that the iron denitrification functional microorganisms could significantly enhance the removal of nitrate and OFL by ZVI/FeS2. When the mass ratio and total dosage of ZVI/FeS2 were 2:1 and 16g/L, respectively, and the inoculation amount of M was 5.81g/L, the nitrate in the ZVI/FeS2+M reaction system was almost completely removed within 168h, and the removal rate of OFL was as high as 90.44%. Meanwhile, the ZVI/FeS2+M system showed a good adaptability at different temperatures(10~30℃), nitrate concentrations(25~150mg/L), OFL concentrations(500ng/L~200ug/L) and the presence of coexisting ions. The intervention of microorganisms greatly delayed the passivation and oxidation of the ZVI surface, and at the same time, it could increase the dissolution of FeS2 to produce FeS, Fe2+ and S2-, which were all beneficial to the removal of pollutants. The removal of nitrate and OFL by ZVI/FeS2+M after six cycles was still kept at 73.80% and 79.25%, which was still higher than that of the ZVI/FeS2 (62.73% and 60.60%) in a one cycle experiment, indicating that the coupled system(ZVI/FeS2+M) is a feasible method for remediating nitrate and antibiotic-containing groundwater.
关键词
零价铁/黄铁矿 /
脱氮微生物 /
地下水污染修复 /
硝酸盐 /
氧氟沙星
Key words
ZVI/FeS2 /
denitrification microorganism /
groundwater pollution remediation /
nitrate /
ofloxacin
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基金
国家自然科学基金资助项目(42377083);四川省自然科学基金项目(2025ZNSFSC0433);四川省大学生创新创业计划项目(S202410621125)
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