聚乳酸微塑料与砷复合污染对蚯蚓的毒性效应

闫艺萌; 刘俊慧; 马琳燕; 冯晓雪; 李柏村; 李欣妍; 曹秀凤; 朱丽; 王志峰

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (8) : 4646-4655.

新污染物与毒理

聚乳酸微塑料与砷复合污染对蚯蚓的毒性效应

闫艺萌, 刘俊慧, 马琳燕, 冯晓雪, 李柏村, 李欣妍, 曹秀凤, 朱丽, 王志峰

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Toxicological effects of polylactic acid microplastics and arsenic co-contamination in earthworms

YAN Yi-meng, LIU Jun-hui, MA Lin-yan, FENG Xiao-xue, LI Bai-cun, LI Xin-yan, CAO Xiu-feng, ZHU Li, WANG Zhi-feng

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摘要

将赤子爱胜蚓(Eisenia fetida)暴露于聚乳酸微塑料(PLA MPs,0.5% w/w)与不同浓度亚砷酸盐(As(Ⅲ),40或100mg/kg)的单一及复合污染土壤中28d,分析蚯蚓体内6种生物标志物的响应特征和两种污染物的生物富集水平,以评估PLA MPs和As(Ⅲ)的联合毒性效应及相关机制.生物富集分析结果显示,复合暴露组的As(Ⅲ)生物富集量较单一As(Ⅲ)暴露组平均下降34.2%.生物标志物分析结果显示,随着暴露水平和时间的增加,蚯蚓体内出现显著的氧化应激响应.采用综合生物标志物响应指数(IBR)量化多种生化指标的综合毒性效应,在PLA MPs和As(Ⅲ)的单一及复合污染组中均显示出显著的剂量-效应关系,且发现复合暴露组的IBR指数值较单一As(Ⅲ)暴露组平均降低28.8%.联合毒性评价结果表明,两者在不同As浓度暴露下的联合毒性效应均为拮抗作用.本研究揭示了PLA MPs与As复合污染对蚯蚓的毒性效应及其潜在机制,为评估此类联合毒性的风险提供了科学依据.

Abstract

In this study, the earthworm Eisenia fetida was exposed to soils contaminated with polylactic acid microplastics (PLA MPs, 0.5% w/w) and arsenite (As(III), 40 or 100mg/kg) under single and co-exposure scenarios for 28 days. The responses of six selected biomarkers and the bioaccumulation levels of the two pollutants in earthworms were analyzed to evaluate the joint toxic effects and underlying mechanisms of PLA MPs and As(Ⅲ). Bioaccumulation results indicated that the bioaccumulation of As(III) in co-exposure groups decreased by an average of 34.2% compared to the single As(III) exposure groups. Biomarker analysis revealed significant oxidative stress responses in earthworms, which intensified with increasing exposure concentration and duration. The Integrated Biomarker Response index (IBR) was used to quantify the integrated toxic effects of multiple biomarkers, showing a significant dose-effect relationship in both single and co-exposure groups. Notably, the IBR values in co-exposure groups were reduced by an average of 28.8% compared to those in single As(III) exposure groups. Joint toxicity evaluation indicated antagonistic interactions between PLA MPs and As(III) at both arsenic exposure levels. These findings systematically elucidate the toxicological effects and potential mechanisms of PLA MPs and As co-contamination in earthworms, providing scientific evidence for ecological risk assessment of such combined toxicity.

导出引用

闫艺萌, 刘俊慧, 马琳燕, 冯晓雪, 李柏村, 李欣妍, 曹秀凤, 朱丽, 王志峰. 聚乳酸微塑料与砷复合污染对蚯蚓的毒性效应[J]. 中国环境科学. 2025, 45(8): 4646-4655

YAN Yi-meng, LIU Jun-hui, MA Lin-yan, FENG Xiao-xue, LI Bai-cun, LI Xin-yan, CAO Xiu-feng, ZHU Li, WANG Zhi-feng. Toxicological effects of polylactic acid microplastics and arsenic co-contamination in earthworms[J]. China Environmental Science. 2025, 45(8): 4646-4655

中图分类号： X503.2

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