冷冲击包埋ANAMMOX颗粒低温脱氮性能分析

吴文婧; 曹业启; 张晶; 张林华; 贺洁雅; 王国钊; 张佩; 郑翔; 陈建伟

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (9) : 4945-4954.

水污染与控制

冷冲击包埋ANAMMOX颗粒低温脱氮性能分析

吴文婧¹, 曹业启¹, 张晶¹, 张林华¹, 贺洁雅², 王国钊¹, 张佩¹, 郑翔¹, 陈建伟¹

作者信息 +

Analysis of the low-temperature nitrogen removal performance of cold-shocked immobilized ANAMMOX granules

WU Wen-jing¹, CAO Ye-qi¹, ZHANG Jing¹, ZHANG Lin-hua¹, HE Jie-ya², WANG Guo-zhao¹, ZHANG Pei¹, ZHENG Xiang¹, CHEN Jian-wei¹

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摘要

针对厌氧氨氧化(ANAMMOX)工艺低温脱氮困难的问题,本研究通过冷冲击成功强化了ANAMMOX包埋颗粒污泥在不同低温下的脱氮性能(R3).结果表明:23~6°C下,R3颗粒的脱氮效率显著高于未冷冲击的包埋颗粒(R2)和未包埋的ANAMMOX污泥(R1);尤其在12和6°C下,R3的优势更加显著.R3颗粒比ANAMMOX活性(SAA)和生物量浓度(MLVSS)较高是其维持低温脱氮效率的关键.12和6°C下,R3的SAA是R1的20~22倍、R2的12~14倍;R3的MLVSS高达6059~4732mg/L.这与冷冲击改善了功能菌的代谢增殖、胞外聚合物(EPS)调节能力和EPS中蛋白质(PN)二级结构有关.值得注意的是,R3内Candidatus Brocadia相对丰度显著提高,使其在极端低温下依然能够保持一定的活性,而颗粒内ANAMMOX菌属和相关耐冷菌属丰度的增加利于调节EPS分泌,提高生物聚集能力.本研究为促进ANAMMOX工艺的低温应用提供了有效的方法.

Abstract

This study addresses the challenge of low-temperature nitrogen removal in the anaerobic ammonium oxidation (ANAMMOX) process. By using cold shock, the nitrogen removal performance of immobilized ANAMMOX particles (R3) at various low temperatures was successfully improved. The results showed that, at the low temperatures ranging from 23°C to 6°C, the nitrogen removal efficiency of R3 particles was significantly higher than that of non-cold-shocked immobilized particles (R2) and non-immobilized ANAMMOX sludge (R1), with the advantage being especially pronounced at 12 and 6°C. The higher specific ANAMMOX activity (SAA) and biomass concentration (MLVSS) of R3 particles were key factors in maintaining efficient nitrogen removal at low temperatures. At 12 and 6°C, the SAA of R3 was 20~22 times higher than that of R1 and 12~14 times higher than R2; the MLVSS of R3 reached as high as 6059~4732mg/L. This was attributed to the fact that cold shock improved the metabolic proliferation of functional bacteria, extracellular polymeric substance (EPS) regulation, and the secondary structure of proteins (PN) in EPS. Notably, the relative abundance of Candidatus Brocadia in R3 was significantly increased, enabling it to maintain activity at extremely low temperatures. Additionally, the increase in the abundance of ANAMMOX genera and cold-tolerant bacterial genera within the particles facilitated the regulation of EPS secretion and improved bioaggregation. This study provides an effective approach for promoting the low-temperature application of the ANAMMOX process.

导出引用

吴文婧, 曹业启, 张晶, 张林华, 贺洁雅, 王国钊, 张佩, 郑翔, 陈建伟. 冷冲击包埋ANAMMOX颗粒低温脱氮性能分析[J]. 中国环境科学. 2025, 45(9): 4945-4954

WU Wen-jing, CAO Ye-qi, ZHANG Jing, ZHANG Lin-hua, HE Jie-ya, WANG Guo-zhao, ZHANG Pei, ZHENG Xiang, CHEN Jian-wei. Analysis of the low-temperature nitrogen removal performance of cold-shocked immobilized ANAMMOX granules[J]. China Environmental Science. 2025, 45(9): 4945-4954

中图分类号： X703

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