铜绿假单胞菌对聚二甲基硅氧烷无害化处理及酶促降解机制

杨世芳; 侯前朋; 刘云鹏; 谢军; 尚海洋

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (9) : 4975-4985.

固体废物

铜绿假单胞菌对聚二甲基硅氧烷无害化处理及酶促降解机制

杨世芳, 侯前朋, 刘云鹏, 谢军, 尚海洋

作者信息 +

Mechanism of harmless treatment and enzymatic degradation of polydimethylsiloxane by pseudomonas aeruginosa

YANG Shi-fang, HOU Qian-peng, LIU Yun-peng, XIE Jun, SHANG Hai-yang

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文章历史 +

摘要

聚二甲基硅氧烷广泛应用于日用、医疗及电力行业,为实现其无害化处理,探索了微生物绿色降解方法,分析了该过程中反应产物的热解特性、组成成分、产生机制以及微生物细胞膜的表面疏水性(CSH).结果表明,利用铜绿假单胞菌株(CGMCC 0452)对聚二甲基硅氧烷(PDMS)进行处理时,CGMCC 0452菌株对高粘度PDMS具有良好耐受性.随着降解天数增加,CSH提高至16%,傅里叶红外在1712cm^-1处检测到鼠李糖脂质链化学结构中羰基的CO伸缩振动,凝胶渗透色谱分析表明PDMS重均分子量与数均分子量分别下降了29130Da和21701Da.基于以上结果并结合气相色谱质谱联用综合分析,确定聚硅氧烷长链主要被降解为环硅氧烷低聚物(D5,D6,D8,D9),降解产物未出现有毒有害物质.最后从微观层面揭示了CSH提高机理(鼠李糖脂吸附和细胞膜脂多糖损失)和环硅氧烷低聚物生成机理.为聚硅氧烷的高效无害化处理提供了新的研究思路,为研究聚合物的生物降解机制提供了参考.

Abstract

Polydimethylsiloxane (PDMS) is widely applied in daily-use products, medical treatment and power industry. In order to achieve its harmless disposal, the microbial green degradation approach was explored, and the pyrolysis characteristics, composition, production mechanism and surface hydrophobicity (CSH) of microbial cell membrane during the degradation process were analyzed. Results demonstrated that CGMCC 0452 strain exhibited strong tolerance to high-viscosity PDMS when polydimethylsiloxane (PDMS) was treated with Pseudomonas aeruginosa strain (CGMCC 0452). With the extension of degradation days, the CSH increased to 16%, and the CO stretching vibration of the carbonyl group in the chemical structure of the rhamnolipid chain was detected at 1712 cm^-1 by Fourier transform infrared spectroscopy. Gel permeation chromatography analysis revealed that the weight-average molecular weight and number-average molecular weight of PDMS decreased by 29130 Da and 21701 Da, respectively. Based on the above results, combined with comprehensive analysis of gas chromatography-mass spectrometry, it was determined that the long chain of polysiloxane was mainly degraded into cyclosiloxane oligomers (D5, D6, D8, D9), and the degradation products did not appear toxic and harmful substances. Finally, the CSH enhancement mechanism (rhamnolipid adsorption and cell membrane lipopolysaccharide loss) and cyclosiloxane oligomer formation mechanism were revealed from the micro level. This paper provides a novel research idea for the efficient and harmless treatment of polysiloxane, and offers a valuable reference for investigating the biodegradation mechanism of polymers.

导出引用

杨世芳, 侯前朋, 刘云鹏, 谢军, 尚海洋. 铜绿假单胞菌对聚二甲基硅氧烷无害化处理及酶促降解机制[J]. 中国环境科学. 2025, 45(9): 4975-4985

YANG Shi-fang, HOU Qian-peng, LIU Yun-peng, XIE Jun, SHANG Hai-yang. Mechanism of harmless treatment and enzymatic degradation of polydimethylsiloxane by pseudomonas aeruginosa[J]. China Environmental Science. 2025, 45(9): 4975-4985

中图分类号： X705

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