高效多环芳烃降解菌Pseudomonas jessenii QYQ-1的分离、鉴定及基因组分析

秦银秋; 王乙橙; 张慕琦; 陈猷鹏

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (9) : 5163-5175.

环境微生物

高效多环芳烃降解菌Pseudomonas jessenii QYQ-1的分离、鉴定及基因组分析

秦银秋, 王乙橙, 张慕琦, 陈猷鹏

作者信息 +

Isolation, identification and genome analysis of Pseudomonas jessenii QYQ-1, a highly efficient polycyclic aromatic hydrocarbon degrading bacterium

QIN Yin-qiu, WANG Yi-cheng, ZHANG Mu-qi, CHEN You-peng

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摘要

从嘉陵江河床沉积物分离获得一株多环芳烃(PAHs)降解菌株QYQ-1,经16S rRNA序列比对､平均核苷酸一致性值计算与全基因组系统发育分析,鉴定其为杰氏假单胞菌(Pseudomonas jessenii).菌株QYQ-1具有广泛的温度(15~40℃)和pH值(5~9)适应范围、优良的耐盐性(0.5%~5%)和良好的重金属抗性(Cu²⁺、Zn²⁺、Co²⁺、Mn²⁺、Cd²⁺),尤其具有耐低温(15℃)、耐高盐(5%)和耐重金属的独特优势,其最佳降解条件为温度35℃,pH值为7,可在48h内对100mg/L萘降解率达到97.7%.此外,其还可以高效降解苯甲酸和苯酚等芳香族化合物.基因组分析表明,菌株QYQ-1具有多种PAHs降解过程中的关键基因,包括完整的苯甲酸盐降解与邻苯二酚邻位裂解通路,还含有水杨酸羟化酶、细胞色素P450、原儿茶酸3,4-双加氧酶等有关PAHs降解的关键基因.同时,还携带大量铜、锌、钴、锰、镉等重金属抗性基因.此外,菌株QYQ-1还具有强大的铁摄取系统.这拓展了菌株QYQ-1的应用范围,不仅可以用于PAHs污染场地的环境修复,还具有应用于重金属和PAHs复合污染场地环境修复的潜力.

Abstract

A strain of polycyclic aromatic hydrocarbons (PAHs) degrading bacteria, QYQ-1, was isolated from the sediments of the Jialing River bed. Through 16S rRNA sequence alignment, calculation of average nucleotide identity, and whole-genome phylogenetic analysis, it was identified as Pseudomonas jessenii. The strain QYQ-1 exhibits a wide adaptive range for temperature (15~40°C) and pH (5~9), excellent salt tolerance (0.5%~5%), and good heavy metal resistance (Cu²⁺, Zn²⁺, Co²⁺, Mn²⁺, Cd²⁺). It has a unique advantage in withstanding low temperatures (15°C), high salinity (5%), and heavy metals. The optimal degradation conditions are at a temperature of 35°C and a pH of 7, achieving a degradation rate of 97.7% for 100mg/L naphthalene within 48hours. Additionally, it can efficiently degrade aromatic compounds such as benzoic acid and phenol. Genomic analysis indicates that the strain QYQ-1 possesses various key genes involved in the degradation of multiple PAHs, including a complete pathway for benzoate degradation and a meta-cleavage pathway for catechol. It also contains critical genes related to PAHs degradation, such as salicylate hydroxylase, cytochrome P450, and protocatechuate 3,4-dioxygenase. Additionally, it carries a significant number of resistance genes for heavy metals such as copper, zinc, cobalt, manganese, and cadmium. Furthermore, strain QYQ-1 has a robust iron uptake system. This expands the application range of strain QYQ-1, making it suitable not only for environmental remediation of PAHs-contaminated sites but also for potential use in environments contaminated with both heavy metals and PAHs.

导出引用

秦银秋, 王乙橙, 张慕琦, 陈猷鹏. 高效多环芳烃降解菌Pseudomonas jessenii QYQ-1的分离、鉴定及基因组分析[J]. 中国环境科学. 2025, 45(9): 5163-5175

QIN Yin-qiu, WANG Yi-cheng, ZHANG Mu-qi, CHEN You-peng. Isolation, identification and genome analysis of Pseudomonas jessenii QYQ-1, a highly efficient polycyclic aromatic hydrocarbon degrading bacterium[J]. China Environmental Science. 2025, 45(9): 5163-5175

中图分类号： X172

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