磺胺间甲氧嘧啶胁迫下硫自养反硝化菌群的响应机制

黄凯文; 张欣; 周雨晨; 周一杰; 王硕; 李激

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (9) : 5227-5235.

新污染物

磺胺间甲氧嘧啶胁迫下硫自养反硝化菌群的响应机制

黄凯文¹, 张欣¹, 周雨晨¹, 周一杰¹, 王硕^1,2, 李激^1,2

作者信息 +

Response mechanisms of sulfur autotrophic denitrifying microbial communities under the stress of sulfamethoxazole

HUANG Kai-wen¹, ZHANG Xin¹, ZHOU Yu-chen¹, ZHOU Yi-jie¹, WANG Shuo^1,2, LI Ji^1,2

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摘要

采用硫填料床反应器,以工业废弃物白泥作为碱度源,逐步提升磺胺间甲氧嘧啶(SMM)浓度,探究硫自养反硝化(SADN)菌群在SMM胁迫下的微观响应机制.结果表明,SADN菌群能够利用白泥提供的碱度进行高效脱氮,在低浓度SMM胁迫下系统保持稳定, Thiobacillus菌群得到富集,硝态氮(NO₃^--N)及SMM去除率达99%以上.在高浓度SMM冲击下,系统中Thiobacillus菌群丰度降低,脱氮性能有所波动.但系统中优势菌群通过增加C6-HSL分泌,调控谷氨酸(Glu)和脯氨酸(Pro)等疏水性氨基酸合成,促进胞外聚合物(EPS)中紧密结合型蛋白(TB-PN)的合成,维持蛋白质二级结构紧密,从而促进污泥絮体团聚.在此调控下,系统脱氮性能恢复,SMM去除率维持在60%以上.此外,在高浓度SMM胁迫下,unclassified_f__Rhodocyclaceae借助凋亡的自养菌群及EPS分泌的碳源协同Ferritrophicum、Thiobacillus等SADN菌群进行高效脱氮.

Abstract

A sulfur packed-bed reactor was employed, with lime mud-an industrial by-product, serving as an alkalinity source. The concentration of Sulfamethoxazole (SMM) was gradually increased to investigate the microscopic response mechanisms of sulfur autotrophic denitrifying (SADN) microbial communities under SMM stress. The results indicated that the SADN microbial communities could utilize the alkalinity provided by lime mud to achieve efficient nitrogen removal. Under low-concentration SMM stress, the system remained stable, Thiobacillus was enriched with removal efficiencies of nitrate (NO₃^--N) and SMM exceeding 99%. Under the impact of high-concentration SMM, the abundance of Thiobacillus in the system decreased, and the nitrogen removal performance fluctuated. However, the dominant bacteria in the system regulated the synthesis of hydrophobic amino acids glutamic acid (Glu) and proline (Pro) by increasing the secretion of C6-HSL, promoted the synthesis of tightly bound protein (TB-PN) in extracellular polymer (EPS), maintained the tight secondary structure of the protein, and thus promoted the aggregation of floc sludge. Under this regulation, the denitrification performance was recovered, and the SMM removal rate reached 60%. Additionally, under high-concentration SMM stress, unclassified_f__Rhodocyclaceae utilized the carbon sources secreted by apoptotic autotrophic bacteria and EPS to synergize with Ferritrophicum and Thiobacillus for efficient nitrogen removal.

导出引用

黄凯文, 张欣, 周雨晨, 周一杰, 王硕, 李激. 磺胺间甲氧嘧啶胁迫下硫自养反硝化菌群的响应机制[J]. 中国环境科学. 2025, 45(9): 5227-5235

HUANG Kai-wen, ZHANG Xin, ZHOU Yu-chen, ZHOU Yi-jie, WANG Shuo, LI Ji. Response mechanisms of sulfur autotrophic denitrifying microbial communities under the stress of sulfamethoxazole[J]. China Environmental Science. 2025, 45(9): 5227-5235

中图分类号： X703

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