碳酸盐矿化菌对喀斯特地区土壤滤液重金属的矿化吸附机制

杨茜; 李小艺; 曹俊毅; 潘云凤; 和成忠; 涂春霖; 吴龙华; 梁新然; 湛方栋

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中国环境科学 ›› 2026, Vol. 46 ›› Issue (1) : 29-40.

岩溶关键带重金属污染成因

碳酸盐矿化菌对喀斯特地区土壤滤液重金属的矿化吸附机制

杨茜¹, 李小艺¹, 曹俊毅¹, 潘云凤¹, 和成忠², 涂春霖², 吴龙华³, 梁新然¹, 湛方栋¹

作者信息 +

Mineralization and adsorption mechanism of carbonate mineralizing bacteria on heavy metals in soil filtratein karst area

YANG Qian¹, LI Xiao-yi¹, CAO Jun-yi¹, PAN Yun-feng¹, HE Cheng-zhong², TU Chun-lin², WU Long-hua³, LIANG Xin-ran¹, ZHAN Fang-dong¹

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文章历史 +

摘要

从喀斯特地区土壤中分离出一株碳酸盐矿化菌C7-1,鉴定为粘质沙雷氏菌(Serratia marcescens),通过单因素实验结合表征技术,解析其对镉(Cd)的去除机制,并探讨其对土壤滤液中Cd、铅(Pb)、锌(Zn)、铜(Cu)的去除方式.结果表明:尿素浓度是调控Cd²⁺去除的关键因素,去除率最高可达74%,其中胞外沉淀(42%)为主要途径,表面吸附(27%)次之;扫描电镜-能谱 (SEM-EDS)、傅里叶红外光谱(FTIR)和X射线衍射(XRD)结果发现Cd²⁺可能与CO₃^2-结合形成CdCO₃.土壤滤液中,菌株C7-1对Cd²⁺、Pb²⁺、Zn²⁺和Cu²⁺的去除率在22.9%~65.9%之间.重金属离子以共沉淀的方式形成无定形颗粒状物质,并在羟基、羧基、碳酸根、磷酸和硫酸基团的作用下吸附在菌体表面.

Abstract

A carbonate-mineralizing bacterial strain C7-1was isolated from karst soil and identified as Serratia marcescens. Through single factor test combined with characterization technology, the cadmium (Cd) removal efficiency and underlyingmechanism of S. marcescens were analyzed, along with its ability toremove Cd, lead (Pb), zinc (Zn), and copper (Cu) from soil. The results indicated that the urea concentration was the main factor controlling Cd²⁺ removal.The maximum Cd²⁺ removal rate reached 74%, which was primarily via extracellular precipitation (42%) followed by surface adsorption (27%). Cd²⁺ may bind to CO₃^2- to form CdCO₃ based on scanning electron microscope-energy dispersive spectroscopy (SEM-EDS), fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The removal efficiencies of strain C7-1 for Cd²⁺, Pb²⁺, Zn²⁺ and Cu²⁺ achieved 22.9%~65.9% in soil filtrates. The heavy metal ions were found to form amorphous mineral precipitates through co-precipitation and were adsorbed onthe bacterial surfaces via functional groups, including hydroxyl, carboxyl, carbonate, phosphoric acid, and sulfuric acid groups.

导出引用

杨茜, 李小艺, 曹俊毅, 潘云凤, 和成忠, 涂春霖, 吴龙华, 梁新然, 湛方栋. 碳酸盐矿化菌对喀斯特地区土壤滤液重金属的矿化吸附机制[J]. 中国环境科学. 2026, 46(1): 29-40

YANG Qian, LI Xiao-yi, CAO Jun-yi, PAN Yun-feng, HE Cheng-zhong, TU Chun-lin, WU Long-hua, LIANG Xin-ran, ZHAN Fang-dong. Mineralization and adsorption mechanism of carbonate mineralizing bacteria on heavy metals in soil filtratein karst area[J]. China Environmental Science. 2026, 46(1): 29-40

中图分类号： X53

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