一株赖氨酸芽孢杆菌对盐酸土霉素降解效能及途径

李佳美; 康宗利; 马骜雨; 赵恩佑; 王虹玲; 杨玉红; 杜立宇

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中国环境科学 ›› 2026, Vol. 46 ›› Issue (2) : 868-879.

土壤污染与控制

一株赖氨酸芽孢杆菌对盐酸土霉素降解效能及途径

李佳美¹, 康宗利¹, 马骜雨¹, 赵恩佑¹, 王虹玲², 杨玉红¹, 杜立宇³

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Degradation efficiency and pathways of oxytetracycline hydrochloride by Lysinibacillus sp.

LI Jia-mei¹, KANG Zong-li¹, MA Ao-yu¹, ZHAO En-you¹, WANG Hong-ling², YANG Yu-hong¹, DU Li-yu³

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摘要

从污染土壤中筛选出盐酸土霉素(OTC-HCl)高效降解菌株OTC-L20,经16S rDNA鉴定并命名为Lysinibacillus sp. OTC-L20,为目前已报道的Lysinibacillus属中具有高效降解OTC-HCl能力的菌株之一.基因组测序发现其染色体含四环素类抗生素、氨基糖苷类抗生素等多种耐药基因,可能参与OTC-HCl的生物转化.OTC-L20在特定条件(外加碳源添加量4g/L、pH = 8、接种量3%、初始底物浓度60mg/L)下,第7d对OTC-HCl的降解率达到79.80%.利用UPLC-HRMS测定到14种主要中间降解产物,通过脱酰胺、烯醇酮异构化等7种方式,分为4条降解路径.与空白对照组相比,OTC-L20降解产物对Escherichia coli和Staphylococcus aureus的生理毒性显著降低,表明其为高效安全盐酸土霉素降解菌.

Abstract

A highly efficient oxytetracycline hydrochloride (OTC-HCl)-degrading strain, designated as Lysinibacillus sp. OTC-L20, was isolated from contaminated soil. Its taxonomic status was confirmed via 16S rDNA sequencing, and it represent a highly efficient OTC-HCl-degrading strain within the genus Lysinibacillus. Genomic sequencing revealed that its chromosome harbors multiple antibiotic resistance genes, including those conferring resistance to tetracyclines and aminoglycosides, which may be involved in the biotransformation of OTC-HCl. Under specific culture conditions (supplemented carbon source at 4g/L, initial pH 8, inoculum size 3%, and initial substrate concentration 60mg/L), the strain achieved a degradation rate of 79.80% for OTC-HCl on the 7th day of incubation. Using ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS), a total of 14major intermediate metabolites were identified, and four distinct degradation pathways were proposed, involving seven reaction types such as deamidation and enol-keto isomerization. Compared with the blank control group, the degradation products of OTC-L20exhibited significantly reduced antibacterial toxicity to Escherichia coli and Staphylococcus aureus, demonstrating that Lysinibacillus sp. OTC-L20is a highly efficient and environmentally safe OTC-HCl-degrading bacterium.

导出引用

李佳美, 康宗利, 马骜雨, 赵恩佑, 王虹玲, 杨玉红, 杜立宇. 一株赖氨酸芽孢杆菌对盐酸土霉素降解效能及途径[J]. 中国环境科学. 2026, 46(2): 868-879

LI Jia-mei, KANG Zong-li, MA Ao-yu, ZHAO En-you, WANG Hong-ling, YANG Yu-hong, DU Li-yu. Degradation efficiency and pathways of oxytetracycline hydrochloride by Lysinibacillus sp.[J]. China Environmental Science. 2026, 46(2): 868-879

中图分类号： X53

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