基于大体积固相萃取的天然淡水溶解性有机质提取及组成表征

胡浩杰; 何晨; 潘娜; 王飏荣; 王一达; 宋桂雪; 白雪松; 阮振鑫; 温佳鑫; 王亚琦; 史权

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中国环境科学 ›› 2026, Vol. 46 ›› Issue (2) : 888-897.

环境生态

基于大体积固相萃取的天然淡水溶解性有机质提取及组成表征

胡浩杰¹, 何晨¹, 潘娜², 王飏荣¹, 王一达¹, 宋桂雪³, 白雪松², 阮振鑫², 温佳鑫², 王亚琦², 史权¹

作者信息 +

Large-volume solid phase extraction-based extraction and compositional characterization of natural freshwater dissolved organic matter

HU Hao-jie¹, HE Chen¹, PAN Na², WANG Yang-rong¹, WANG Yi-da¹, SONG Gui-xue³, BAI Xue-song², RUAN Zhen-xin², WEN Jia-xin², WANG Ya-qi², SHI Quan¹

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文章历史 +

摘要

溶解性有机质(DOM)是水生生态系统和全球碳循环的关键组分,但淡水内源DOM参考样品的缺乏,制约了对其环境行为和生物地球化学循环的深入理解.本研究旨在提供一种克级高纯度淡水内源DOM参考样品.通过放大的改性苯乙烯-二乙烯基苯聚合物树脂填充柱固相萃取法,处理南水北调中线工程8t河水,成功制备了13.12g DOM,命名为NSBD-DOM.综合运用元素分析、傅立叶变换红外光谱、三维荧光光谱、核磁共振波谱和高分辨质谱等手段,系统表征了NSBD-DOM的化学组成,并与国际腐殖酸协会提供的苏万尼河富里酸(SRFA)进行对比.结果表明,NSBD-DOM主体为类富里酸组分,包含碳水化合物端基碳、烯烃/共轭烯烃以及氨基糖等特征.相较于SRFA,NSBD-DOM的分子量、缩合度、氧元素含量较低,其中多酚类和多环芳烃类化合物含量较低;然而,其氢碳比、氮含量、不饱和脂肪族化合物和高度不饱和化合物含量较高.NSBD-DOM具备典型内源特征,可为DOM的环境行为、碳循环等研究提供参考.

Abstract

Dissolved organic matter (DOM) is a key component of aquatic ecosystems and the global carbon cycle. However, the lack of reference samples for endogenous freshwater DOM has hindered a deeper understanding of its environmental behavior and biogeochemical cycling. This study aims to provide a gram-scale, high-purity endogenous freshwater DOM reference sample. By employing an amplified solid-phase extraction method using modified styrene-divinylbenzene polymer resin-packed columns, 8tons of water from the central line project of South-to-North Water Diversion were processed, successfully yielding 13.12g of DOM, named as NSBD-DOM. A comprehensive suite of analytical techniques—including elemental analysis, Fourier transform infrared spectroscopy, excitation-emission matrix spectra, nuclear magnetic resonance spectroscopy, and high-resolution mass spectrometry—was employed to systematically compare NSBD-DOM with Suwannee River Fulvic Acid (SRFA) from the International Humic Substances Society. The NSBD-DOM was predominantly composed of fulvic acid like components, exhibiting characteristic of carbohydrate anomeric carbon, alkenes/conjugated alkenes, and amino sugars. Compared to SRFA, NSBD-DOM exhibited lower molecular weight, condensation degree, and oxygen content, accompanied by lower levels of polyphenols and polycyclic aromatics. However, it showed a higher H/C ratio and higher nitrogen content, with elevated levels of unsaturated aliphatic compounds and highly unsaturated compounds. NSBD-DOM exhibits typical endogenous characteristics, it can serve as a unified reference for studies on DOM’s environmental behavior, carbon cycling, and related research fields.

导出引用

胡浩杰, 何晨, 潘娜, 王飏荣, 王一达, 宋桂雪, 白雪松, 阮振鑫, 温佳鑫, 王亚琦, 史权. 基于大体积固相萃取的天然淡水溶解性有机质提取及组成表征[J]. 中国环境科学. 2026, 46(2): 888-897

HU Hao-jie, HE Chen, PAN Na, WANG Yang-rong, WANG Yi-da, SONG Gui-xue, BAI Xue-song, RUAN Zhen-xin, WEN Jia-xin, WANG Ya-qi, SHI Quan. Large-volume solid phase extraction-based extraction and compositional characterization of natural freshwater dissolved organic matter[J]. China Environmental Science. 2026, 46(2): 888-897

中图分类号： X132

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