大气CO2浓度升高对水库水华优势绿藻生长及光合作用的影响

陶首仲; 张顾篷; 张陵蕾; 李嘉; 赵阳; 王宏伟; 陈旻

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中国环境科学 ›› 2026, Vol. 46 ›› Issue (2) : 898-909.

环境生态

大气CO₂浓度升高对水库水华优势绿藻生长及光合作用的影响

陶首仲¹, 张顾篷², 张陵蕾¹, 李嘉¹, 赵阳³, 王宏伟³, 陈旻¹

作者信息 +

Effects of elevated atmospheric CO₂ concentration on the growth and photosynthesis of dominant green algae in reservoir algal blooms

TAO Shou-zhong¹, ZHANG Gu-peng², ZHANG Ling-lei¹, LI Jia¹, ZHAO Yang³, WANG Hong-wei³, CHEN Min¹

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文章历史 +

摘要

本文以常见的水库水华优势绿藻—小球藻(Chlorella vulgaris)为研究对象,通过自制CO₂浓度恒定装置,模拟400μmol/mol(现状)、800μmol/mol(预测2100年)和2000μmol/mol(极端)3种CO₂浓度条件,于恒温光照培养箱中开展了为期35d的室内实验,探究CO₂浓度升高对藻类生长、光合活性及固碳能力的动态响应.结果表明:实验短期(24d内)培养下高CO₂浓度(800,2000μmol/mol)会显著刺激藻密度增长(最高达972.8×10⁶cell/L),但长期(35d)培养下高CO₂浓度会抑制小球藻的增殖,缩短其生命周期;光合活性参数(F_v/F_m、rETR)对CO₂浓度变化不敏感,但大气CO₂浓度升高会抑制小球藻的生长从而间接导致其光合效率降低;增殖阶段(24d内)藻群整体固碳量随CO₂浓度升高而增加(极端条件下达35.919mgC),但个体细胞固碳效率下降;长期(35d)高CO₂浓度条件下,固碳量呈现负值,藻群由"碳汇"转变为"碳源",反而会加剧CO₂的释放.研究揭示了CO₂浓度升高对绿藻短期促进增殖、长期抑制生长的双阶段效应,并阐明其对水库碳循环的潜在影响.

Abstract

This paper focuses on the common dominant green alga in reservoirs algal blooms, Chlorella vulgaris, as the research subject. Using a homemade CO₂ concentration stabilizing device, we simulated three CO₂ concentration conditions: 400 μmol/mol (current status), 800μmol/mol (predicted for 2100), and 2000μmol/mol (extreme). A 35-day indoor experiment was conducted in a constant temperature light incubator to systematically investigate the dynamic responses of algal growth, photosynthetic activity, and carbon fixation capacity to elevated CO₂ concentrations. The results indicated that: In the short-term cultivation (within 24d), high CO₂ concentrations (800 and 2000μmol/mol) significantly stimulated algal density growth, reaching up to 972.8×10⁶cells/L. However, in the long-term cultivation (35d), high CO₂ concentrations inhibited the proliferation of Chlorella vulgaris and shortened its lifespan. The photosynthetic activity parameters (F_v/F_m, rETR) were not sensitive to changes in CO₂ concentration. However, elevated atmospheric CO₂ levels inhibited the growth of Chlorella vulgaris, indirectly leading to a reduction in its photosynthetic efficiency. During the proliferation phase (within 24d), the overall carbon fixation of the algal population increased with rising CO₂ concentrations, reaching up to 35.919mg C under extreme conditions. However, the carbon fixation efficiency per cell decreased. In the long term (35d) under high CO₂ concentrations, the carbon fixation turned negative, transforming the algal population from a "carbon sink" to a "carbon source," thereby exacerbating CO₂ release. The study revealed the dual-phase effects of elevated CO₂ concentrations on green algae, with short-term promotion of proliferation and long-term inhibition of growth.

导出引用

陶首仲, 张顾篷, 张陵蕾, 李嘉, 赵阳, 王宏伟, 陈旻. 大气CO₂浓度升高对水库水华优势绿藻生长及光合作用的影响[J]. 中国环境科学. 2026, 46(2): 898-909

TAO Shou-zhong, ZHANG Gu-peng, ZHANG Ling-lei, LI Jia, ZHAO Yang, WANG Hong-wei, CHEN Min. Effects of elevated atmospheric CO₂ concentration on the growth and photosynthesis of dominant green algae in reservoir algal blooms[J]. China Environmental Science. 2026, 46(2): 898-909

中图分类号： X524

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