Abstract:PCR-DGGE analysis was used to study the diversity of the microbial community in different anaerobic fermentation systems using chicken dung, pig manure, cow dung and straw as substrate respectively. Methane biogas production and methane concentration were measured daily. Slurry was sampled at different fermentation stage and used for DGGE analysis. The results showed that the overall trend of daily biogas production appeared pig> chicken> straw> cow dung and their average biogas production was 2.67, 2.24, 0.99, 0.49L. Daily methane concentration of pig, chicken manure and straw was above 50% during the whole fermenting period, while the methane concentration of cow was less than 30% most of the time. Dominant bacterial groups were Bacteroidetes, Treponema, Anaerolineaceae and the newly developed bacterial groups were Clostridium, Desulfobulbus, Lachnospiraceae and Acetivibrio. Dominant archaeal groups were Methanobacteriaceae, Methanosarcina and Methanosarcinales. A newly developed group was Methanosphaera stadtmanae.
李海红, 巴琦玥, 闫志英, 刘晓风. 不同原料厌氧发酵及其微生物种群的研究[J]. 中国环境科学, 2015, 35(5): 1449-1457.
LI Hai-Hong, BA Qi-Yue, YAN Zhi-Ying, LIU Xiao-Feng. Studies on microbial community of different materials and anaerobic fermentation. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(5): 1449-1457.