Abstract：The effects of nonionic surfactant Tween60 and anionic surfactant SDS on bioremediation of DDT contaminated soils by white rot fungus Phlebia lindtneri GB1027 were investigated. The results showed that Tween 60 and SDS could enhance the degradation rate of DDT by fungus in soil, and the optimum concentration of both surfactants for fungal degradation of DDT was 1.0mg/g. P. lindtneri GB1027 showed the higher degradation activity of DDT from soil with Tween60than that with SDS. The efficiency of Tween60-SDS mixed surfactants for enhancing biodegradation capacity was as: Tween60-SDS (3:1)>Tween60-SDS (2:1)>Tween60-SDS (1:1), and the enhancing capacity of Tween60-SDS (3:1) and Tween60-SDS (2:1) for fungal degradation of DDT from soil was higher than that of Tween 60. The inoculum amount could greatly enhance the removal rate of DDT from soil by P. lindtneri GB1027at a range of 0.2 to 1.0mL/g, and the highest degradation rate of DDT was 70.9% within 30days, when the inoculum amount of fungus was 1.0mL/g. It was also found that fungal degradation rates of DDT increased sharply with increasing soil moisture at a range between 10% and 50%, and that DDT of about 70% was removed from soil with moisture of 50% during 30 days of incubation. The above results confirmed the workability of white rot fungus-surfactants augmented remediation of DDT-contaminated soil.
