Characteristics of tetracycline resistance genes in the anaerobic and aerobic treatment of tetracycline production wastewater
REN Jia1, YAO Hong1, LIU Miao-miao1, ZHANG Yu2, YANG Min2
1. Department of Civil Engineering, Beijing Jiaotong University, Beijing 100044, China;
2. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
In order to understand the characteristics of antibiotic resistance genes (ARGs) in the anaerobic and aerobic treatment processes of two tetracycline production wastewater, the abundances and distribution of six frequently reported tetracycline resistance genes (tet(A), tet(C), tet(G), tet(Q), tet(W), and tet(X)) and two types of mobile elements (intI1 and ISCR3) were determined by PCR and quantitative PCR (qPCR). tet(C) was detected in none of the samples, while the others were discovered in all of the samples. In the systems of the tetracycline production wastewater, the relative abundances (normalized to 16S rRNA genes) of tet(A), tet(G), tet(X) in the anaerobic sludge((1.25±0.16)×10-4~(4.52±0.002)×10-2) were lower than those in the aerobic sludge ((9.88±0.67)×10-5~(2.70±0.29)×10-1), while the relative abundances of tet(Q) and tet(W) in the anaerobic sludge ((1.66±0.03)×10-2~(7.48±1.22)×10-2) were significantly higher than those in the aerobic sludge (1.94±0.12)×10-3~(2.85±0.16)×10-2). Besides, the relative abundances of intI1 and ISCR3 in the anaerobic sludge ((1.48±0.01)×10-3~(2.61±0.31)×10-2) were significantly lower than those in the aerobic sludge ((1.18±0.15)×10-1~(8.99±0.75)×10-1), showing that the potential of horizontal gene transfer mediated by the mobile elements is lower in the anaerobic treatment process. It is indicated that aerobic treatment may facilitate the spread of tet(A), tet(G) and tet(X) but control the spread of tet(Q) and tet(W) in tetracycline production wastewater, with the opposite situation in the anaerobic treatment. The different distribution of tet genes was related to mobile genetic elements, resistance mechanism, and community structure.
任佳, 姚宏, 刘苗苗, 张昱, 杨敏. 厌氧和好氧处理过程中四环素抗药基因的丰度[J]. 中国环境科学, 2016, 36(1): 268-275.
REN Jia, YAO Hong, LIU Miao-miao, ZHANG Yu, YANG Min. Characteristics of tetracycline resistance genes in the anaerobic and aerobic treatment of tetracycline production wastewater. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(1): 268-275.
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