A bioflocculant was produced from slaughter wastewater, and the performances in methylene blue wastewater treatment were investigated by using the bioflocculant alone or combined with chitosan. Furthermore, response surface methodology (RSM) was employed to optimize the treatment process by the composite of this bioflocculant and chitosan. Results showed that the optimal culture conditions for bioflocculant production were 2g urea, 2g glucose, 2g K2HPO4, and 1g KH2PO4 dissolved in 1L slaughter wastewater. The corresponding bioflocculant yield reached 2.92g/L after fermentation for 60h at 35℃ and 150r/min. For the methylene blue wastewater with a concentration of 20mg/L, when the bioflocculant was adjusted to 15mg/L and the solution pH was 7, removal efficiency of methylene blue reached 64.9%. Optimal conditions for methylene blue removal by the composite of bioflocculant and chitosan were bioflocculant dose of 12.9mg/L, chitosan dose of 0.07g/L, and pH=6. Under this optimal condition, removal efficiency of methylene blue appeared as 94.7%, indicated that it is a feasible way to significantly promoted methylene blue wastewater treatment by using the composite of bioflocculant and chitosan, and the effluent quality was meeting to the 1A discharge standard of the discharge standard of pollutants for municipal wastewater treatment plant (GB 18918-2002).
郭俊元, 陈诚, 刘文杰. 微生物絮凝剂及与壳聚糖复配处理亚甲基蓝废水[J]. 中国环境科学, 2017, 37(9): 3346-3352.
GUO Jun-yuan, CHEN Cheng, LIU Wen-jie. A bioflocculant and its performances in treatment of methylene blue wastewater by composited with chitosan. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(9): 3346-3352.
Aljubooria A H R, Idrisa A, Norhafizah A, et al. Production and characterization of a bioflocculant produced by Aspergillus flavus[J]. Bioresource Technology, 2013,127:489-493.
[3]
Yi T, Lee E H, Ahn Y G. Novel biodegradation pathways of cyclohexane by Rhodococcus sp. EC1[J]. Journal of Hazardous Materials, 2011,191:393-396.
[4]
More T T, Yan S, Hoang N V, et al. Bacterial polymer production using pre-treated sludge as raw material and its flocculation and dewatering potential[J]. Bioresource Technology, 2012,121:425-431.
[5]
Pu S Y, Qin L L, Che J P, et al. Preparation and application of a novel bioflocculant by two strains of Rhizopus sp. using potato starch wastewater as nutrilite[J]. Bioresource Technology, 2014, 162:184-191.
[6]
Amit B, Mika S. Applications of chitin-and chitosan-derivatives for the detoxification of water and wastewater-A short review[J]. Advances in Colloid and Interface Science, 2009,152:26-38.
Yang Z H, Huang J, Zeng G M, et al. Optimization of flocculation conditions for kaolin suspension using the composite flocculant of MBFGA1 and PAC by response surface methodology[J]. Bioresource Technology, 2009,100:4233-4239.
[10]
Guo J Y, Yang C P, Zeng G M. Treatment of swine wastewater using chemically modified zeolite and bioflocculant from activated sludge[J]. Bioresource Technology, 2013,143:289-297.
[11]
Guo J Y, Lau A K, Zhang Y Z, et al. Characterization and flocculation mechanism of a bioflocculant from potato starch wastewater[J]. Applied Microbiology and Biotechnology, 2015,99:5855-5861.
[12]
Nwodo U U, Agunbiade M O, Ezekiel G, et al. A freshwater Streptomyces, isolated from Tyume River, produces a predominantly extracellular glycoprotein bioflocculant[J]. International Journal of Molecular Sciences, 2012,13:8679-8695.
Sarayu M, Shalini S, Jyoti D. Response surface methodology for optimization of medium for decolorization of textile dye direct black 22by a novel bacterial consortium[J]. Bioresource Technology, 2007,99:562-569.
[15]
Kavita K, Mishra A, Jha B. Extracellular polymeric substances from two biofilm forming Vibrio species:Characterization and applications[J]. Carbohydrate Polymers, 2013,94:882-888.
[16]
Ahmad H, Rajab A, Azni I, et al. Production and characterization of a bioflocculant produced by Aspergillus flavus[J]. Bioresource Technology, 2013,127:489-493.
[17]
You Y, Ren N, Wang A, et al. Use waste fermenting liquor to produce bioflocculants with isolated strains[J]. International Journal of Hydrogen Energy, 2008,33:3295-3301.
[18]
Li Z, Zhong S, Lei H Y, et al. Production of a novel bioflocculant by Bacillus licheniformis X14and its application to low temperature drinking water treatment[J]. Bioresource Technology, 2009,100:3650-3656.
[19]
陈湘.壳聚糖的改性及其在印染废水处理中的应用[D]. 苏州:苏州大学, 2008.
[20]
潘媛媛.磁性壳聚糖的改性及其对模拟废水的处理研究[D]. 太原:中北大学, 2013.
[21]
Wang Y Q, Liu S J, Xu Z, et al. Ammonia removal from leachate solution using natural Chinese clinoptilolite[J]. Journal of Hazardous Materials, 2006,136:735-740.
[22]
Gong W X, Wang S G, Sun X F, et al. Bioflocculant production by culture of serratia ficaria and its application in wastewater treatment[J]. Bioresource Technology, 2008,99:4668-4674.
Liu X, Li X M, Yang Q, et al. Landfill leachate pretreatment by coagulation-flocculation process using iron-based coagulants:Optimization by response surface methodology[J]. Chemical Engineering Journal, 2012,200:39-51.
[25]
Guo J Y, Yu J, Xin X, et al. Characterization and flocculation mechanism of a bioflocculant from hydrolyzate of rice stover[J]. Bioresource Technology, 2015,177:393-397.