The environmental evolution research of regional groundwater using molecular biotechnologies: a case from the shallow groundwater in front of the Taihang Mountains
HE Ze1,2, ZHANG Min1, NING Zhuo1,2, XIANG Xiao-ping1,2, LIU Jun-Jian1,2, HOU Qin-xuan1,2, ZHAO Qian1,2
1. Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang 050061, China; 2. Key Labratory of Groundwater Remediation, China Geological Survey/Hebei, Shijiazhuang 050803, China
Abstract：The shallow groundwater in Dasha Rivers Basin was selected as the study subject, because of it is recognized as one of the typical regions in front of the Taihang Mountains. 94samples were collected along the river. The 16s rDNA gene sequences were tested by high-throughput sequencing technologies. According to the environmental factors of NO3-, COD and DO, the microbial communities and the functional indicator microorganisms related to environmental evolution were studied. The results showed that, using the cumulative probability distribution method, the samples were divided into four groups:background, nitrate pollution, organic pollution and organic-nitrate pollution (B group, N group, O group, O_N group). This grouping rules were close to the I and Ⅱ water grade in the quality standard of groundwater. The pollution driven the microbial communities to converge, and the organic pollution could decrease the richness diversity of microbial communities. The functional indicator microorganisms related to environmental evolution in the different pollution region were summarized as followed:organic pollution could be indicated by Micromonospora and unclassified_f_Micromonosporaceae, nitrate pollution could be indicated by Chryseobacterium and Streptomyces, organic-nitrate pollution could be indicated by Pseudomonas and Microvirgula. The molecular biotechnologies and analysis methods used in this research could provide the theoretical evidence for region environmental investigations and bioremediation.
何泽, 张敏, 宁卓, 向小平, 刘俊建, 侯钦宣, 赵谦. 区域地下水环境演化的分子生物学特征——以太行山前平原浅层水为例[J]. 中国环境科学, 2019, 39(8): 3484-3492.
HE Ze, ZHANG Min, NING Zhuo, XIANG Xiao-ping, LIU Jun-Jian, HOU Qin-xuan, ZHAO Qian. The environmental evolution research of regional groundwater using molecular biotechnologies: a case from the shallow groundwater in front of the Taihang Mountains. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(8): 3484-3492.
石建省,王昭,张兆吉,等.华北平原地下水有机污染特征初步分析[J]. 生态环境学报, 2011,20(11):1695-1699. Shi Jiansheng, Wang Zhao, Zhang Zhaoji et al. Preliminary analysis on the organic contamination of groundwater in the North China Plain[J]. Ecology & Environmental Sciences, 2011,20(11):1695-1699.
[2]
黄金玉,姜月华,苏晶文,等.长江三角洲地区地下水调查点特殊分布的评价方法解析[J]. 地质学报, 2016,90(10):2948-2961. Huang Jinyu, Jiang Yuehua, Su Jingwen et al. Analysis of the Special Distribution of Groundwater Survey Points in the Yangtze River Delta[J]. Acta Geologica Sinica, 2016,90(10):2948-2961.
[3]
刘景涛,孙继朝,黄冠星,等.区域地下水污染调查评价野外调查方法浅议[J]. 地下水, 2008,30(2):60-61. Liu Jingtao, Sun Jichao, Huang Guanxing et al. Discussion of Field Survey Methods of the Regional Groundwater Pollution Investigation Appraisal[J]. Ground Water, 2008,30(2):60-61.
[4]
赵晖,刘淼,李瑞.淮河流域地下水资源调查评价与利用研究[J]. 地下水, 2010,32(3):44-47. Zhao Hui, Liu Miao, Li Rui. Investigation and evaluation and study on the utilization of the groundwater resources of Huaihe Watershed the groundwater resources of Huaihe Watershed[J]. Ground Water, 2010,32(3):44-47.
[5]
文冬光,林良俊,孙继朝,等.区域性地下水有机污染调查与评价方法[J]. 中国地质, 2008,35(5):814-819. Wen Dongguang, Lin Liangjun, Sun Jichao et al. Approach to investigation and assessment of organic contaminants in regional groundwater[J]. Geology in China, 2008,35(5):814-819.
[6]
DZ/T 0288-2015区域地下水污染调查评价规范[S]. DZ/T 0288-2015 Specification for regional groundwater contamination survey and evaluation[S].
[7]
John D E, Rose J B. Review of factors affecting microbial survival in groundwater[J]. Environmental Science & Technology. 2005,39(19):7345-7356.
[8]
宁卓,郭彩娟,蔡萍萍,等.某石油污染含水层降解能力地球化学评估[J]. 中国环境科学, 2018,38(11):4068-4074. Ning Zhuo, Guo Caijuan, Cai Pingping et al. Geochemical evaluation of biodegradation capacity in a petroleum contaminated aquifer[J]. China Environmental Science, 2018,38(11):4068-4074.
[9]
李元杰,王森杰,张敏,等.土壤和地下水污染的监控自然衰减修复技术研究进展[J]. 中国环境科学, 2018,38(3):1185-1193. Li YuanJie, Wang Sen Jie, Zhang Min et al. Research progress of monitored natural attenuation remediation technology for soil and groundwater pollution[J]. China Environmental Science, 2018,38(3):1185-1193.
[10]
Scow K M, Hicks K A. Natural attenuation and enhanced bioremediation of organic contaminants in groundwater[J]. Current Opinion in Biotechnology, 2005,16(3):246-253.
[11]
Bekins B A, Cozzarelli I M, Godsy E M, et al. Progression of natural attenuation processes at a crude oil spill site:Ⅱ. Controls on spatial distribution of microbial populations[J]. Journal of Contaminant Hydrology, 2001,53(3):387-406.
[12]
Karczewski K, Riss H W, Meyer E I. Comparison of DNA-fingerprinting (T-RFLP) and high-throughput sequencing (HTS) to assess the diversity and composition of microbial communities in groundwater ecosystems[J]. Limnologica-Ecology and Management of Inland Waters, 2017,67:45-53.
[13]
Dewettinck T, Hulsbosch W, Hege K, et al. Molecular fingerprinting of bacterial populations in groundwater and bottled mineral water[J]. Applied Microbiology & Biotechnology, 2001,57(3):412-418.
[14]
Gavrilescu M, Demnerová K, Aamand J, et al. Emerging pollutants in the environment:present and future challenges in biomonitoring, ecological risks and bioremediation[J]. New biotechnology, 2015, 32(1):147-156.
[15]
Bruno A, Sandionigi A, Galimberti A, et al. One step forwards for the routine use of high-throughput DNA sequencing in environmental monitoring. An efficient and standardizable method to maximize the detection of environmental bacteria[J]. Microbiology Open, 2017, 6(1):e00421.
Ning Z, Zhang M, He Z, et al. Spatial Pattern of Bacterial Community Diversity Formed in Different Groundwater Field Corresponding to Electron Donors and Acceptors Distributions at a Petroleum-Contaminated Site[J]. Water, 2018,10(7):842.
[18]
Zheng Z, Zhang Y, Su X, et al. Responses of hydrochemical parameters, community structures, and microbial activities to the natural biodegradation of petroleum hydrocarbons in a groundwater-soil environment[J]. Environmental Earth Sciences, 2016,75(21):1400.
[19]
黄小兰,陈建耀,周世宁,等.小流域地下水与地表水微生物组成结构差异及其成因分析[J]. 中国环境科学, 2012,32(9):1647-1654. Huang Xiaolan, Chen Jianyao, Zhou Shining et al. Cause on the differences of microorganism between small watershed surface water and underground water[J]. China Environmental Science, 2012,32(9):1647-1654.
[20]
王光生,杨建青,于钋,等.地下水动态预测的探讨[J]. 水文, 2013, 33(3):25-28. Wang Guangsheng, Yang Jianqing, Yu, Po. et al. Preliminary Study on Groundwater Level Prediction[J]. Journal of China Hydrology, 2013, 33(3):25-28.
[21]
马新双,卢胜勇,李明良,等.北方浅层地下水超采区地下水预测模型[J]. 南水北调与水利科技, 2011,9(4):134-136. Ma Xinshuang, Lu Shengyong, Li MingLiang et al. Research of Groundwater Prediction Model in Shallow Groundwater Over-Exploitation Zone in the Northern Area[J]. South-to-North Water Diversion and Water Science & Technology, 2011,9(4):134-136.
[22]
王昭,石建省,张兆吉,等.污水河地区地下水土中苯系物分布特征分析——以太行山前平原为例[J]. 安徽农业科学, 2013,41(12):5537-5538. Wang Zhao, Shi Jiansheng, Zhang Zhaoji et al. Distribution Characteristics of BTEX in Groundwater and Soil in the Region of Wastewater River——A Case Study of the Piedmont Plain of Taihang Mountains[J]. Journal of Anhui Agricultural Sciences, 2013,41(12):5537-5538.
[23]
昌盛,耿梦娇,刘琰,等.滹沱河冲洪积扇地下水中多环芳烃的污染特征[J]. 中国环境科学, 2016,36(7):2058-2066. Chang Sheng, Geng Mengjiao, Liu Yan et al. Pollution characteristic of polycyclic aromatic hydrocarbons in the groundwater of Hutuo River Pluvial Fan[J]. China Environmental Science, 2016,36(7):2058-2066.
[24]
Zhou J, Xia B, Treves D S, et al. Spatial and resource factors influencing high microbial diversity in soil[J]. Apply Environmental Microbiology, 2002,68(1):326-334.
[25]
He Z, Ning Z, Yang M, et al. The Characterization of Microbial Communities Response to Shallow Groundwater Contamination in Typical Piedmont Region of Taihang Mountains in the North China Plain[J]. Water, 2019,11(4):736.
[26]
Gojgic-Cvijovic G, Milic J, Solevic T, et al. Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium:a laboratory study[J]. Biodegradation, 2012,23(1):1-14.
[27]
Arafa M A. Biodegradation of some aromatic hydrocarbons (BTEXs) by a bacterial consortium isolated from polluted site in Saudi Arabia[J]. Pak. J. Biol. Sci, 2003,6(17):1482-1486.
[28]
Kundu P, Pramanik A, Dasgupta A, et al. Simultaneous heterotrophic nitrification and aerobic denitrification by Chryseobacterium sp. R31isolated from abattoir wastewater[J]. BioMed research international, 2014,2014:1-12.
[29]
Kumon Y, Sasaki Y, Kato I, et al. Codenitrification and denitrification are dual metabolic pathways through which dinitrogen evolves from nitrate in Streptomyces antibioticus[J]. Journal of Bacteriology, 2002, 184(11):2963-2968.
[30]
Patureau D, Helloin E, Rustrian E, et al. Combined phosphate and nitrogen removal in a sequencing batch reactor using the aerobic denitrifier, Microvirgula aerodenitrificans[J]. Water Research, 2001, 35(1):189-197.
