Biodegradation of Sulfamethazine in Sulfamethazine/Copper combined pollution by Sakaguchia cladiensis
ZENG Jie-yi1, ZENG Su-hang1, JI Meng-tian1, TAN Xiao2, HAN Wei-jiang1,2, CHEN Shuo-na1
1. College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China;
2. South China Institute of Environmental Science, Ministry of Ecology and Environment, Guangzhou 510655, China
To understand the characteristics of sulfamethazine (SMZ) biodegradation in the polluted environment where SMZ and Cu2+ co-existed, this study focused on the bio-treatment of SMZ-Cu2+ in different conditions by Sakaguchia cladiensis (named A5), a strain resistant to sulfamethazine, which was screened and isolated from the soil of a livestock and poultry farm (Guangzhou, China). The results showed that, with the presence of single SMZ or SMZ-Cu2+ combined pollution in the culture medium, strain A5 still maintained a good growth trend. Sakaguchia cladiensis was an effective strain to remove SMZ from pollution system. The presence of Cu2+ was beneficial for A5 to decompose SMZ. The degradation rates of SMZ reached 37.3% in 3d. Under the condition of 1g/L of inoculum, 1mg/L initial concentration of SMZ, and neutral pH, the removal effect of strain A5 on SMZ was the best. The transmission electron microscope (TEM) analysis showed that the cells of strain A5 were severely damaged when SMZ and Cu2+ co-existed in solution, leading to the outflow of cytoplasm.
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