Toxicity and mechanism of ZnO-NPs on an aerobic denitrifying bacteria strain Zobellella sp.B307
WU Chun-han1, BAI Jie1,2, ZHAO Yang-guo1,2, REN Zhao-meng1, CHEN Lin1, LI Kui-ran3
1. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; 2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; 3. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Abstract：In this paper, a strain of aerobic denitrifying bacteria Zobellellasp. B307screened from the sediment of Jiaozhou Bay was studied. Under short-term exposure condition, the toxic effect of ZnO-NPs on the bacterial strain was analysed through the changes in growth, denitrification ability, related enzyme activity and metabolic pathway of the bacteria. The toxicity mechanism of ZnO-NPs on the strain was discussed based on the dissolution test of zinc ions and the oxidative stress levels such as of CAT and ROS. The results showed that comparing with the control group, the addition of ZnO-NPs at 200mg/L could decrease the nitrate removal rate and NIR activity of the strain to 57.53% and 14.46%. Whereas it could increase the LDH、ROS level、SOD activity to 378%、534% and 60.32% respectively. ZnO-NPs could induce the formation of reactive oxygens species and through that, the membrane permeability of strain was changed, the enzyme activity was decreased, and the related pathway of protein, amino acid synthesis and gene expression were affected, which inhibited the denitrification ability of the strain. The production of free zinc ions is not the main cause of toxicity of ZnO-NPs on the bacterial strain.
张立德.我国纳米材料技术应用的现状和产业化的机遇[J].材料导报, 2001,15(7):2-5. Zhang L D. Current situation of nanomaterials technology application and Opportunities for Industrialization in China[J]. Materials Reports, 2001,15(7):6-9.
[2]
朱世东,周根树,蔡锐,等.纳米材料国内外研究进展Ⅰ--纳米材料的结构、特异效应与性能[J].热处理技术与装备, 2010,31(3):1-5,26. Zhu S D, Zhou G S, Cai R, et al. Nanomaterials research progress at home and abroadⅠ-structure, the specific effect and properties of nanometer materials[J]. Heat Treatment Technology and Equipment, 2010,31(3):1-5,26.
[3]
周全法.纳米材料的应用和产业化[J].江苏技术师范学院学报, 2002,8(4):83-87,107. Zhou Q F. Application and industrialization of nanomaterials[J]. Journal of Jiangsu Teachers University of Technology, 2002,8(4):83-87,107.
[4]
Maynard A D, Aitken R J, Butz T, et al. Safe handling of nanotechnology[J]. Nature, 2006,444(7117):267-269.
[5]
Boxall A, Chaudhry Q, Sinclair C, et al. Current and future predicted environmental exposure to engineered nanoparticles[M]. London:Central Science Laboratory, York, UK, 2007.
[6]
Ge Y, Schimel J P, Holden P A. Evidence for negative effects of TiO2 and ZnO nanoparticles on soil bacterial communities[J]. Environmental Science & Technology, 2011,45(4):1659-1664.
[7]
潘少兵,邱凤仙,余世金.纳米氧化锌毒性研究进展[J].科学技术创新, 2016,110(33):116-116. Pan S B, Qiu F X, Yu S J. Research progress of toxicity of nano-zinc oxide[J]. Scientific and Technological Innovation, 2016,110(33):116-116.
[8]
李小义,王丽萍,杜雅萍,等.好氧反硝化微生物多样性及其反硝化功能初步研究[J].氨基酸和生物资源, 2016,38(2):37-45. Li X Y, Wang L P, Du Y P, et al. Microbial diversity and denitrification function of aerobic denitrification[J]. Amino Acids and Biotic Resources, 2016,38(2):37-45.
[9]
梁书诚,赵敏,卢磊,等.好氧反硝化菌脱氮特性研究进展[J].应用生态学报, 2010,21(6):1581-1588. Liang S C, Zhao M, Lu L, et al. Research progress of nitrogen removal characteristics of aerobic denitrifying bacteria[J]. Chinese Journal of Applied Ecology, 2010,21(6):1581-1588.
[10]
白洁,陈琳,黄潇,等.1株耐盐异养硝化-好氧反硝化菌Zobellella sp. B307的分离及脱氮特性[J].环境科学, 2018,39(10):403-411. Bai J, Chen L, Huang X, et al. Isolation and denitrification of a strain of salt-tolerant heterotrophic nitrification aerobic denitrification bacterium Zobellella SP. B307[J]. Environmental Science, 2018, 39(10):403-411.
[11]
Kristjansson J K, Hollocher T C. First practical assay for soluble nitrous oxide reductase of denitrifying bacteria and a partial kinetic characterization[J]. Journal of Biological Chemistry, 1980,255(2):704-707.
[12]
杨立志,王鹏胤,胡南.M.hydrocarbonoclasticus NY4中nosZ基因的克隆与异源表达[J].华中农业大学学报, 2017,36(2):64-68. Yang L Z, Wang P Y, Hu N, et al. Cloning and heterologous expression of nos Z gene in M. hydrocarbonoclasticus NY4[J]. Journal of Huazhong Agricultural University, 2017,36(2):64-68.
[13]
Zhang L, Manthiram A.Chains composed of nanosize metal particles and identifying the factors driving their formation[J]. Applied Physics Letters, 1997,70(18):2469-2471.
[14]
Ma H, Williams P L, Diamond S A. Ecotoxicity of manufactured ZnO nanoparticles-a review[J]. Environmental Pollution, 2013,172(1):76-85.
[15]
Roselli M, Finamore A, Garaguso I, et al. Zinc oxide protects cultured enterocytes from the damage induced by escherichia coli[J]. The Journal of Nutrition, 2003,133(12):4077-4082.
[16]
Li M, Lin D, Zhu L. Effects of water chemistry on the dissolution of ZnO nanoparticles and their toxicity to Escherichia coli[J]. Environmental Pollution, 2013,173(2):97-102.
[17]
Xie Y, He Y, Irwin P L, et al. Antibacterial activity and mechanism of action of zinc oxide nanoparticles against campylobacter jejuni[J]. Applied and Environmental Microbiology, 2011,77(7):2325-2331.
[18]
王树涛,陈玲波,张真瑞,等.氧化锌纳米颗粒对污泥厌氧消化过程的影响研究[J].中国环境科学, 2017,37(1):174-180. Wang S T, Chen L B, Zhang Z R, et al. Effects of zno nanoparticles on sludge anaerobic digestion[J]. China Environmental Science, 2017, 37(1):174-180.
[19]
Moura I, Moura J J. Structural aspects of denitrifying enzymes.[J]. Current Opinion in Chemical Biology, 2001,5(2):168-175.
[20]
Granger J, Ward B B. Accumulation of nitrogen oxides in copper-limited cultures of denitrifying bacteria[J]. Limnology and Oceanography, 2003,48(1):313-318.
[21]
Labbe N, Parent S, Villemur R. Addition of trace metals increases denitrification rate in closed marine systems[J]. Water Research, 2003,37(4):914-920.
[22]
Muhammad O, Talha K A, Nazar U I, et al. Role of plant phytochemicals and microbial enzymes in biosynthesis of metallic nanoparticles[J]. Applied Microbiology and Biotechnology, 2018, 102(16):6799-6814.
[23]
李玲丽.纳米材料的生物响应、生物合成及利用[D].中国科学技术大学, 2017. Li L L. Biological response, biosynthesis and utilization of nanomaterials[D]. University of Science and Technology of China, 2017.
[24]
Mei W S, Gang Z C. New insights on the structure and function of glutamyl-prolyl-tRNA synthetase[J]. Bulletin of the Academy of Military Medical Sciences, 2006,30(2):166-168.
[25]
赵琳,陈瑞,陈锋,等.纳米氧化锌毒性效应及毒理学机制研究进展[J].毒理学杂志, 2016,30(2):161-166. Zhao L, Chen R, Chen F, et al. Research progress on the toxic effects and toxicological mechanism of nano-zinc oxide[J]. Journal of Toxicology, 2016,30(2):161-166.
[26]
Pasquet J, Chevalier Y, Couval E, et al. Antimicrobial activityof zinc oxide particles on five microorganisms of the Challenge Tests related to their physicochemical properties[J]. International Journal of Pharmaceutics, 2014,460(2):92-100.
[27]
Sawai J. Quantitative evaluation of antibacterialactivities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay[J]. Journal of Microbiological Methods, 2003,54(2):177-182.
[28]
Kumar A, Pandey A K, Singh S S, et al. Engineered ZnO and TiO2 nanoparticles induce oxidative stress and DNA damage leading to reduced viability of Escherichia coli[J]. Free Radical Biology and Medicine, 2011,51(10):1872-1881.
[29]
田文静,白伟,赵春禄,等.纳米ZnO对斑马鱼胚胎抗氧化酶系统的影响[J].中国环境科学, 2010,30(5):705-709. Tian W J, Bai W, Zhao C L, et al. Effect of Nano-ZnO on Zebrafish embryo antioxidant enzyme system[J]. China Environmental Science, 2010,30(5):705-709.
[30]
Djurisic A B, Leung Y H, Xu X Y, et al. Toxicity of metal oxide nanoparticles:Mechanisms, characterization, and avoiding experimental artifacts[J]. Small, 2015,11(1):26−44.
[31]
Wang D, Zhao L X, Ma H Y, et al. Quantitative analysis of reactive oxygen species photogenerated on metal oxide nanoparticles and their bacteria toxicity:The role of superoxide radicals[J]. Environmental Science & Technology, 2017,51(17):10137-10145.
[32]
Djurisic A B, Leung Y H, Xu X Y, et al. Toxicity of metal oxide nanoparticles:Mechanisms, characterization, and avoiding experimental artifacts[J]. Small, 2015,11(1):26−44.
[33]
An Y, Shen Y B, Zhang Z X. Effects of mechanical damage and herbivore wounding on H2O2 metabolism and antioxidant enzyme activities in hybrid poplar leaves[J]. Journal of Forestry Research, 2009,20(2):156-160.
[34]
王大彬,赵利霞,郭良宏,等.连续流动化学发光法在线定量检测二氧化钛光催化产生的O2·-和H2O2及其生成动力学研究[J].化学学报, 2015,73(5):388-394. Wang D B, Zhao L X, Guo L H, et al. Online quantification of O2·- and H2O2 and their formation kinetics in ultraviolet (UV)-irradiated nano-TiO2 suspensions by continuous flow chemiluminescence[J]. Acta Chimica Sinica, 2015,73(5):388-394.
[35]
Hirakawa T, Nosaka Y, et al. Properties of O2·- and OH·formed in TiO2 aqueous suspensions by photocatalytic reaction and the influence of H2O2 and some ions[J]. Langmuir, 2002,18(18):3247-3254.
