Inhibitionandassociated mechanism of conjugative transfer of β-lactamase resistance genes in ESBLs producing bacteria by chlorine disinfection
ZHANG Chong-miao, NIU Zhi-yao, WANG Zhen, LI Yong-qiang, LIANG Jie
Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Abstract：Extended-spectrum β-lactamases (ESBLs) producing Escherichia coli T413 and Escherichia coli NK5449 were used as the donor and recipient strain, respectively, to study the inhibitory effect of chlorine disinfection of donor strain on conjugative transfer of β-lactamases resistance genes. The results showed that the blaCTX-M and blaTEM geneson the donor plasmid could be transferred into the recipient strainwith a conjugative transfer frequency of 6.57×10-2. When the contact time was 30min and the active chlorine concentration was 0.25~1.5mg/L, the conjugative transfer frequency was not reduced by orders of magnitude, but when the active chlorine concentration was more than 2mg/L, the conjugative transfer frequency declinedsharplyto 2.02×10-5. Among the various combinations for the CT value of 60(mg·min)/L, 4mg/L×15min had the best inhibitory effect on the conjugative transfer. The number of donor strain after chlorine disinfection was positively correlated with the conjugative transfer frequency. The donor straindisinfected by high dose of chlorinehad higher regrowth rate, but the conjugative transfer frequency was very low. High dose chlorine disinfection can damage the structure and function of donor strain, and reduce the extracellular secretion, thereby blocking plasmid transfer.
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