Effect of carbon source types on denitrifying phosphorus removal and microbial community in the A2/O - BCO process
FAN Ya-jun1, ZHANG Miao2, JI Jun-jie2, CHENG Ji-lin1, WU Qi-chao3, HE Cheng-da2
1. College of Hydraulic Science and Engineering, Yangzhou University, Yangzhou 225127, China; 2. MCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China; 3. Yangzhou Polytechnic Institute, Yangzhou 225127, China
Abstract：The anaerobic/anoxic/aerobic-biological contact oxidation (A2/O-BCO) process was used (1) to treat low carbon/nitrogen ratio (C/N) wastewater, (2) to monitor the effect of single factor of carbon source (Phase Ⅰ: sodium acetate; Phase Ⅱ: Sodium acetate + sodium propionate; Phase Ⅲ: sodium propionate) on organic matter, and (3) to investigate the simultaneous nitrogen and phosphorus removals were investigated. The research explored the transformation and utilization of internal carbon sources (PHA and Gly) and the mechanism of denitrifying phosphorus removal (DPR) under the mixed carbon source conditions of sodium acetate and sodium propionate. Meanwhile, the evolution rules of microbial community structure at different phases were compared through high-throughput sequencing. The results showed that the mixed carbon source improved the simultaneous removal efficiency of organic matter, nitrogen and phosphorus, and the transformation amount of internal carbon source was 226mg/h with the phosphorus release amount of 30.58mg/L in the anaerobic section, and the DPR efficiency was above 90%. The batch test showed that the proportion of denitrifying phosphorous accumulating bacteria (DPAOs) to phosphorous accumulating bacteria (PAOs) was 72.42%, which basically realized the enrichment of DPAOs. The results of high-throughput sequencing showed that the mixed carbon sources were more conducive to the formation of unique OTUs bacterial genus, and the total amount of PAOs (including Accumulibacter and Acinetobacter) and DPAOs (including Dechloromonas and Pseudomonas) was up to 29.13%(> 16.18%(Phase Ⅲ) > 14.34%(Phase Ⅰ)), thus efficiently promoting the utilization of carbon source and efficiently denitrifying phosphorus removal. In the BCO reactor, the total amount of ammonia-oxidizing bacteria (AOB, including Nitrosomonas and Nitrosomonadaceae) and nitrite-oxidizing bacteria (NOB, mainly Nitrospira) increased from 3.89%(N1) to 23.09%(N2) and 37.23%(N3), which provided sufficient electron receptors for denitrifying phosphorus removal. In addition, the operation regulation strategy based on the efficient utilization of carbon source was established, in order to provide theoretical reference for the application of A2/O-BCO process.
范亚骏, 张淼, 季俊杰, 程吉林, 吴启超, 何成达. A2/O-BCO系统中碳源类型对反硝化除磷及菌群结构的影响[J]. 中国环境科学, 2022, 42(1): 172-182.
FAN Ya-jun, ZHANG Miao, JI Jun-jie, CHENG Ji-lin, WU Qi-chao, HE Cheng-da. Effect of carbon source types on denitrifying phosphorus removal and microbial community in the A2/O - BCO process. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 172-182.
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