Oxidative stress responseresponse of petroleum-contaminated soil to earthworm
OUYANG Shao-hu1, HUANG Pan-pan2, TAO Zong-xin1, ZHOU Qi-xing1
1. Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China; 2. Environmental Protection Agency, Dongying 257000, China
Abstract：The ecological toxicity of petroleum-contaminated soil of the Yellow River Delta on the earthworm (Eisenia foetida, test organisms) were investigated under 28d toxicity exposure, and then the ecotoxicity threshold of petroleum hydrocarbon in soil environment was preliminarily deduced, which could provide support for soil ecotoxicology diagnosis. The results showed that the activities of antioxidant enzymes and content of MDA in Eisenia foetida had no obvious response to exposure time after exposure to different concentrations (0~10g/kg) petroleum hydrocarbon contaminated soil for 7d, 14d, 21d and 28d. In response to the exposure concentration, the activities of SOD, CAT and POD in earthworms didn't have a uniform trend and couldn't be generalized. However, there was a positive linear correlation between the content of MDA in earthworms and the exposure concentration of petroleum hydrocarbons (R2>0.85). Therefore, MDA content of earthworm could be used as a biomarker to indicate lipid peroxidation under petroleum hydrocarbon contaminated soil stress.
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