Characterization of pyrene's impact on the soil functional microorganisms for nitrogen transformation
HU Qin1, ZHANG Li-lan1,2, YI Mei-ling1, YANG Rui1
1. Key Laboratory of Three Gorges Reservoir Region's Eco-environment, Ministry of Education, Chongqing University, Chongqing 400044, China; 2. State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China
Abstract：An aerobic degradation microenvironment was constructed to analyze the effects of pyrene (12.09mg/kg) at ambient concentration on soil enzyme activities, the whole process of nitrogen transformation and related functional microorganisms. The results showed that pyrene only significantly increased urease activity on the first day of degradation, but promoted the dehydrogenase activity at both the early and late phases of degradation. The analysis of the bacterial community structure revealed that the variation of the relative abundance of ammonia-oxidizing bacteria (Nitrososphaeraceae) promoted pyrene-mediated aerobic ammonia oxidation and nitrification in the early stages of treatment and inhibited that in the late stages. In contrast, the effects of nitrogen-fixing bacteria (Bradyrhizobium, Mesorhizobium and Ensifer), urea-degrading bacteria (Roseomonas), and nitrate-reducing bacteria (Opitutus) were opposite. The quantitative analysis of functional genes showed that, despite pyrene's repressive effect on the nitrogen-fixing gene nifH at the start of the culture, the abundance of nifH showed an increasing trend, which was not consistent to the anticipated changes in microbial community structure and associated functions. Compared with changes in key enzyme activities and genes encoding the processes of ammonia oxidation and denitrification, pyrene did not significantly boost ammonia oxidation in the early stages of incubation, t severely hampered ammonia oxidation and denitrification after 15days, significantly inhibited the ammonia oxidation process. In this study, how pyrene influenced the microbial nitrogen transformation process in soil was reported, fundamental data on understanding the environmental hazard of pyrene were provided.
胡琴, 张利兰, 易美玲, 杨锐. 芘对土壤微生物氮转化功能菌群的影响特征[J]. 中国环境科学, 2023, 43(10): 5574-5582.
HU Qin, ZHANG Li-lan, YI Mei-ling, YANG Rui. Characterization of pyrene's impact on the soil functional microorganisms for nitrogen transformation. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(10): 5574-5582.
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