硝酸盐异化还原为铵耦合厌氧氨氧化处理含氮废水

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (3353 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要为探究厌氧氨氧化(Anammox)细菌同时去除废水中铵(NH₄⁺)和硝酸盐(NO₃^-)的可行性及长期去除效果,基于Anammox菌的内源硝酸盐异化还原为铵过程,进行了批式实验、抑制剂实验、同位素实验以及长期反应器实验.结果表明,在MLVSS为4500mg/L的批式实验中,经12h反应后NH₄⁺和NO₃^-被同时去除并生成了N₂,总氮去除率为93.49%.在乙炔(抑制Anammox反应中铵和一氧化氮生成肼的过程)抑制实验中,检测到17.01mg N/L的NH₄⁺,Anammox菌对于NO₃^-去除的贡献率为85.88%.当加入青霉素G(抑制异养反硝化菌活性)后,NO₃^-仍可以被去除.在¹⁴NH₄⁺和¹⁵NO₃^-同位素示踪实验中一直能够检测到²⁹N₂和³⁰N₂的生成,前者是后者含量的1.41倍.在长期实验中,反应器稳定运行阶段平均出水NH₄⁺和NO₃^-浓度分别为1.18和2.86mg N/L,平均总氮去除率为92.26%.由此可见,Anammox菌通过内源硝酸盐异化还原为铵耦合常规Anammox过程,可以长期稳定地同时去除废水中NH₄⁺和NO₃^-.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	赵硕
	汪超
	杨蒙
	乔森

关键词 ：厌氧氨氧化, 硝酸盐异化还原为铵, 铵, 硝酸盐

Abstract：To investigate the feasibility and long-term performance of simultaneous removal of ammonium (NH₄⁺) and nitrate (NO₃^-) in wastewater by anaerobic ammonia oxidation (anammox) bacteria, batch, inhibitor, isotopic and long-term reactor experiments were conducted based on the intracellular carbon-dependent dissimilatory nitrate reduction to ammonium (DNRA) of anammox bacteria. In 12h batch experiments, NH₄⁺ and NO₃^- were simultaneously converted to N₂ with anammox biomass concentration of 4500mg/L, and the total nitrogen removal efficiency was 93.49%. In the experiment of acetylene inhibition (inhibiting the process of generating hydrazine from NH₄⁺and nitric oxide in the anammox reaction), 17.01mg N/L of NH₄⁺ was detected, and the contribution rate of anammox bacteria to NO₃^-removal was 85.88%. After the addition of penicillin G (an inhibitor for most heterotrophic denitrifying bacteria), NO₃^- could still be removed. Both ²⁹N₂ and ³⁰N₂ was always detected from the very beginning in the ¹⁵N isotope tracing experiment, and the former was 1.4 folds higher than the latter. In the long-term experiment, the average NH₄⁺ and NO₃^- concentrations of effluent were 1.18 and 2.86mg N/L respectively, reaching an average total nitrogen removal efficiency of 92.26%. These results suggested that NH₄⁺ and NO₃^-could be simultaneously removed by endogenous DNRA coupled with normal anammox metabolism, further promoting the application of anammox-based processes for nitrogen-contained wastewater removal.

Key words： anammox dissimilatory nitrate reduction to ammonium ammonia nitrate

收稿日期: 2024-01-02

PACS:

X703

基金资助:国家自然科学基金项目(22176026)

作者简介: 赵硕(1998-),男,山东聊城人,大连理工大学硕士研究生,研究方向为废水生物处理理论与技术.zhaoshuo9697@163.com.

引用本文:

赵硕, 汪超, 杨蒙, 乔森. 硝酸盐异化还原为铵耦合厌氧氨氧化处理含氮废水[J]. 中国环境科学, 2024, 44(8): 4389-4399. ZHAO Shuo, WANG Chao, YANG Meng, QIAO Sen. Treatment of nitrogen-containing wastewater by dissimilatory nitrate reduction to ammonium coupled with anaerobic ammonium oxidation. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(8): 4389-4399.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I8/4389

[1] Lackner S, Gilbert E M, Vlaeminck S E, et al. Full-scale partial nitritation/anammox experiences: An application survey [J]. Water Research, 2014,55:292-303.
[2] van De Graaf A A, Mulder A, De Bruijn P, et al. Anaerobic oxidation of anammox is a biologically mediated process[J]. Applied and Environmental Microbiology, 1995,61(4):1246-1251.
[3] Wang B, Guo Y, Zhao M, et al. Achieving energy-efficient nitrogen removal and excess sludge reutilization by partial nitritation and simultaneous anammox denitrification and sludge fermentation process [J]. Chemosphere, 2019,218:705-714.
[4] Jetten M S M, Strous M, van de Pas-Schoonen K T, et al. The anaerobic oxidation of ammonium [J]. FEMS Microbiology Reviews, 1998,22(5):421-437.
[5] 韩雪恪,王峥嵘,彭永臻,等.几种重要因素对厌氧氨氧化过程的影响综述[J]. 中国环境科学, 2023,43(5):2220-2227. Han X, Wang Z, Peng Y, et al. A review of several important factors influencing the anammox process [J]. China Environmental Science, 2023,43(5):2220-2227.
[6] Strous M, Kuenen J G, Jetten M S M. Key physiology of anaerobic ammonium oxidation [J]. Applied and Environmental Microbiology, 1999,65(7):3248-3250.
[7] Ma C, Zeng W, Li J, et al. Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process [J]. Science of the Total Environment, 2023,855:158653.
[8] Trinh H P, Lee S, Jeong G, et al. Recent developments of the mainstream anammox processes: Challenges and opportunities [J]. Journal of Environmental Chemical Engineering, 2021,9(4):105583.
[9] Van Hulle S W H, Vandeweyer H J P, Meesschaert B D, et al. Engineering aspects and practical application of autotrophic nitrogen removal from nitrogen rich streams [J]. Chemical Engineering Journal, 2010,162(1):1-20.
[10] 韩可心,张琼,冯岩,等.三级厌氧氨氧化处理生活污水与硝酸盐废水[J]. 中国环境科学, 2022,42(2):637-643. Han K, Zhang Q, Feng Y, et al. Simultaneous removal of domestic wastewater and nitrate wastewater by three-stage anammox system [J]. China Environmental Science, 2022,42(2):637-643.
[11] Cao S, Koch K, Du R, et al. Toward mainstream anammox by integrating side stream treatment [J]. Environmental Science & Technology, 2022,56(15):10553-10556.
[12] Strous M, Pelletier E, Mangenot S, et al. Deciphering the evolution and metabolism of an anammox bacterium from a community genome [J]. Nature, 2006,440:790-794.
[13] Kartal B, Rattray J, van Niftrik L A, et al. Candidatus "Anammoxoglobus propionicus" a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria [J]. Systematic and Applied Microbiology, 2007,30(1):39-49.
[14] Kartal B, Kuypers M M M, Lavik G, et al. Anammox bacteria disguised as denitrifiers: Nitrate reduction to dinitrogen gas via nitrite and ammonium [J]. Environmental Microbiology, 2007,9(3):635-642.
[15] Lam P, Lavik G, Jensen M M, et al. Revising the nitrogen cycle in the Peruvian oxygen minimum zone [J]. Proceedings of the National Academy of Sciences, 2009,106(12):4752-4757.
[16] Wang C, Qiao S. Electron transfer mechanism of intracellular carbon-dependent DNRA inside anammox bacteria [J]. Water Research, 2023,244:120443.
[17] Wang C, Qiao S, Bi Z, et al. Nitrate removal by anammox biomass with intracellular carbon source as electron donors via DNRA pathway [J]. Environmental Research, 2021,200:111390.
[18] Wang C, Qiao S, Zhou J. Nitrate-dependent anaerobic ammonium oxidation [J]. Journal of Cleaner Production, 2023,382:135410.
[19] Castro-Barros C M, Jia M, van Loosdrecht M C M, et al. Evaluating the potential for dissimilatory nitrate reduction by anammox bacteria for municipal wastewater treatment [J]. Bioresource Technology, 2017,233:363-372.
[20] Wang W, Wang T, Liu Q, et al. Biochar-mediated DNRA pathway of anammox bacteria under varying COD/N ratios [J]. Water Research, 2022,212:118100.
[21] Bi Z, Zhang W, Song G, et al. Iron-dependent nitrate reduction by anammox consortia in continuous-flow reactors: A novel prospective scheme for autotrophic nitrogen removal [J]. Science of the Total Environment, 2019,692:582-588.
[22] Kartal B, de Almeida N M, Maalcke W J, et al. How to make a living from anaerobic ammonium oxidation [J]. FEMS Microbiology Reviews, 2013,37(3):428-461.
[23] Gilch S, Vogel M, Lorenz M W, et al. Interaction of the mechanism- based inactivator acetylene with ammonia monooxygenase of Nitrosomonas europaea [J]. Microbiology-SGM, 2009,155(1):279-284.
[24] Hyman M R, Wood P M. Suicidal inactivation and labeliing of anammox mono-oxygenase by acetylene [J]. Biochemical Journal, 1985,227(3):719-725.
[25] Kartal B, Maalcke W J, De Almeida N M, et al. Molecular mechanism of anaerobic ammonium oxidation [J]. Nature, 2011,479(7371):127- 130.
[26] Yoshinari T, Knowles R. Acetylene inhibition of nitrous oxide reduction by denitrifying bacteria [J]. Biochemical and Biophysical Research Communications, 1976,69(3):705-710.
[27] 申海旭,王伟刚,王亚宜.厌氧氨氧化污泥颗粒化机理及快速形成策略[J]. 中国环境科学, 2024,44(4):2011-2022. Shen H, Wang W, Wang Y. Mechanism and acceleration strategy of anammox sludge granulation [J]. China Environmental Science, 2024,44(4):2011-2022.
[28] Li Z, Peng Y, Gao H. Enhanced long-term advanced denitrogenation from nitrate wastewater by anammox consortia: Dissimilatory nitrate reduction to ammonium (DNRA) coupling with anammox in an upflow biofilter reactor equipped with EDTA-2Na/Fe(II) ratio and pH control [J]. Bioresource Technology, 2020,305:123083.
[29] Van De Graaf A A, De Bruijn P, Robertson L A, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor [J]. Microbiology, 1996,142(8):2187-2196.
[30] Okabe S, Oshiki M, Takahashi Y, et al. N₂O emission from a partial nitrification-anammox process and identification of a key biological process of N₂O emission from anammox granules [J]. Water Research, 2011,45(19):6461-6470.
[31] Guo M, Wang C, Qiao S. Light-driven ammonium oxidation to dinitrogen gas by self-photosensitized biohybrid anammox systems [J]. iScience, 2023,26(5):106725.
[32] 金志刚,张彤,朱怀兰.污染物生物降解[M]. 上海:华东理工大学出版社, 1997:57-63. Jin Z, Zhang T, Zhu H. Biodegradation of pollutants [M]. Shanghai.: East China University of Science and Technology Press, 1997:57-63.
[33] 张晓健,黄霞,林金海.农药废水有机物生物降解性能评价及难降解有机物治理对策[J]. 环境科学学报, 1995,15(1):114-121. Zhang X, Huang X, Lin J. Biodegradability assessment of organic pollutants and controlling measures for refractory organics in pesticide wastewater compounds. [J] Acta Scientiae Circumstantiae, 1995,15(1):114-121.
[34] 彭永臻,孙洪伟,杨庆.短程硝化的生化机理及其动力学[J]. 环境科学学报, 2008,28(5):817-824. Peng Y, Sun H, Yang Q. The biochemical mechanism and kinetics of partial nitrification [J]. Acta Scientiae Circumstantiae, 2008,28(5): 817-824.
[35] 王淑莹,孙洪伟,杨庆,等.传统生物脱氮反硝化过程的生化机理及动力学[J]. 应用与环境生物学报, 2008,14(5):732-736. Wang S, Sun H, Yang Q, et al. Biochemical reaction mechanism and kinetics of denitrification [J]. Chinese Journal of Applied and Environmental Biology, 2008,14(5):732-736.
[36] Verdouw H, Van Echteld C J A, Dekkers E M J. Ammonia determination based on indophenol formation with sodium salicylate [J]. Water Research, 1978,12(6):399-402.
[37] Apha. Standard methods for the examination of water and wastewater. 23 rd ed. [M]. Washington, DC.: American Public Health Association, 2017.
[38] Laugier A, Garai J O Z. Derivation of the ideal gas law [J]. Journal of Chemical Education, 2007,84(11):1832-1833.
[39] Ma B, Wang S, Cao S, et al. Biological nitrogen removal from sewage via anammox: Recent advances [J]. Bioresource Technology, 2016,200:981-990.
[40] Pang Y, Wang J. Various electron donors for biological nitrate removal: A review [J]. Science of the Total Environment, 2021,794: 148699.
[41] 常尧枫,郭萌蕾,谢军祥,等.厌氧氨氧化脱氮除碳功能菌群结构及代谢途径[J]. 中国环境科学, 2022,42(3):1138-1145. Chang Y, Guo M, Xie J, et al. The structure and metabolic pathway of functional bacteria for nitrogen and carbon removal in anammox [J]. China Environmental Science, 2022,42(3):1138-1145.
[42] 赵一淳,刘亚雷,李亦舒,等.强污泥流失对anammox工艺脱氮性能及菌群的影响[J]. 中国环境科学, 2022,42(5):2161-2168. Zhao Y, Liu Y, Li Y, et al. Effects of high sludge loss on the nitrogen removal performance and microbial community of anammox process [J]. China Environmental Science, 2022,42(5):2161-2168.
[43] Ma B, Qian W, Yuan C, et al. Achieving mainstream nitrogen removal through coupling anammox with denitratation [J]. Environmental Science & Technology, 2017,51(15):8405-8413.
[44] Arumugham T, Khudzari J, Abdullah N, et al. Research trends and future directions on nitrification and denitrification processes in biological nitrogen removal [J]. Journal of Environmental Chemical Engineering, 2024,12(2):111897.
[45] Lu H, Chandran K, Stensel D. Microbial ecology of denitrification in biological wastewater treatment [J]. Water Research, 2014,64:237- 254.
[46] Wang S, Li J, Wang C W, et al. Reaction of the anammox granules to various antibiotics and operating the anammox coupled denitrifying reactor for oxytetracycline wastetwater treatment [J]. Bioresource Technology, 2022,348:126756.
[47] Li D, Chen H, Gao X, et al. Establishment and optimization of partial nitrification/anammox/partial nitrification/anammox (PN/A/PN/A) process based on multi-stage ammonia oxidation: Using response surface method as a tool [J]. Bioresource Technology, 2022,361: 127722.
[48] Xiao H, Peng Y, Zhang Q, et al. Pre-anaerobic treatment enhanced partial nitrification start-up coupled with anammox for advanced nitrogen removal from low C/N domestic wastewater [J]. Bioresource Technology, 2021,337:125434.
[49] Pandey C B, Kumar U, Kaviraj M, et al. DNRA: A short-circuit in biological N-cycling to conserve nitrogen in terrestrial ecosystems [J]. Science of the Total Environment, 2020,738:139710.